Nikon SMZ25 Instruction Manual page 74

 Filter cube
 P2-EFL GFP-B, P2-EFL GFP-L, and P2-EFL RFP Filter Cubes: Used for fluorescence observation with a given
wavelength.
 P2-EFLC Filter Cube: Used for fluorescence observation with an arbitrary wavelength when combined with arbitrary
filters, for example.
 P2-EFLBF Filter Cube (Bright Field): Used for bright-field episcopic observation.
 Empty cube (Three cubes are supplied with an epi-fluorescence attachment): Used when performing diascopic
illumination observation using the epi-fluorescence attachment.
Light shielding plate
The device is equipped with a light shielding plate for preventing the reflection of ultraviolet light. Use the light shielding
plate when performing fluorescence microscopy. When in use, the light shielding plate can be pushed aside to positions
up to 45° horizontally to the rear. When not in use, the plate can be pushed aside to positions up to 170°.
Bright-field episcopic observation
This is useful for observing the entire image of the opaque specimen during fluorescence observation. Although the
P2-EFLBF Filter Cube contains the UV blocking filter, adjust the brightness of the illuminator so that illumination is not too
bright. When observing light reflected from the specimen with a mirror-like surface, the brightness on the right path differs
from that on the left optical path. Also, duplicated images may be produced by internal half mirror.
OCC light shielding plate
When using an epi-fluorescence attachment with a diascopic illumination base, Nikon recommends closing the OCC light
shielding plate on the dia-illumination completely. This can decrease background noise caused by intrinsic fluorescence in
the diascopic illuminator.
For details about using the light source, refer to the instruction manual for the light source.
Chapter 4
Functions and Operations of the Devices
60