Dic Prism For The Diascopic Illumination - Nikon Eclipse LV100DA-U Instructions Manual

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DIC Prism for the Diascopic Illumination

To perform the DIC microscopy under the diascopic illumination, set the rotatable polarizer for the
diascopic illumination equipped with the 1/4 lambda plate and the analyzer slider at the crossed
Nicols position, and then insert the DIC prism into the front and back of the specimen. To perform
the sensitive color DIC microscopy under the diascopic illumination, insert the lambda plate slider
into the slot on the nosepiece in the configuration of the DIC microscopy under the diascopic
illumination.
The DIC prism to be attached to the condenser is named "condenser DIC prism" and the DIC prism
to be attached to the nosepiece is named "objective DIC prism." Refer to the table on page 67 to
select the DIC prism suitable for the magnification of the used objective or the observation purpose.
Objectives for the DIC microscopy under the diascopic illumination
To perform the DIC microscopy under the diascopic illumination, use the DIC objectives for
biologic microscopes listed in the table on page 67. To attach the DIC objectives to the LV-
NU5AI nosepiece, use the conversion adapter (LU nosepiece adapter M32-25).
Inserting the condenser DIC prism
Attach the condenser DIC prism inside the universal
condenser beforehand. Rotate the condenser turret to
place the condenser DIC prism into the optical path.
There are three types of condenser DIC prisms (N1 Dry,
N2 Dry, and NR Dry). Refer to the table on Page 67 to
select the DIC prism suitable for the used objective.
* For the procedure of attaching the condenser DIC
prism, see the instruction manual for the LV-CUD.
Attaching the objective DIC prism and placing it into the optical path
Attach the objective DIC prism to the slot for objectives
on the nosepiece. To place the DIC prism into the optical
path, insert the DIC slider to the limited position. To
remove the DIC prism from the optical path, detach the
DIC slider from the nosepiece.
Interference color
You can change the interference color continuously by adjusting the orientation of the polarizer.
Polarizer
Dark color
Gray
Sensitive red-violet
66
Characteristics
Observations similar to the dark-field microscopy can be performed.
You can observe the phase contrast distribution of the whole specimen with a bird's eye view.
Observations with the highest color contrast can be performed.
Condenser
turret
Nosepiece
Lambda plate
DIC slider slot
40X I
DIC slider
NC B
ND 8
F.S .