Bright-Field Microscopy - Nikon ECLIPSE TS100 Instructions Manual

Bright-field Microscopy

1-3
Before proceeding to the following operations, perform steps 1 to 6 in "1 Microscopy".
1
Remove the annular diaphragm and slit diaphragm from the
optical path.
If a phase slider or NAMC slider is set, enter an empty hole into the optical path or remove the
slider.
2
Insert an NCB filter. (for TS100/TS100-F only)
Insert an NCB11 filter into the filter holder to change the illumination to the white light. Since
the color temperature differs by the lamp voltage, turn the brightness adjuster till the
background color becomes white. If the viewfield becomes too bright by this adjustment, add an
ND filter to the filter holder.
3
Switch to the desired objective.
Turn the revolving nosepiece to enter the desired objective in the optical path. (Turn the
revolving nosepiece until you feel it click in place.)
4
Adjust the aperture diaphragm.
Adjust the size of the aperture diaphragm so that it is 70% to 80% of the numerical aperture of the
objective.
For details on adjusting the aperture diaphragm, see P.23.
Note that the aperture diaphragm is attached to the condenser and cannot be adjusted if the
condenser has been removed.
3 Microscopy
1-3 Bright-field Microscopy
NCB filter
Filter holder
E-22
Close down to 70% to 80% of
the numerical aperture of the
objective.
Set to an empty hole or remove
the slider.