Nikon eclipse fn1 Instructions Manual
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Nikon eclipse fn1 Instructions Manual

Nikon eclipse fn1 Instructions Manual

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M368E
05.10.NF.1
Microscope
ECLIPSE FN1
Instructions

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  • Page 1 M368E 05.10.NF.1 Microscope ECLIPSE FN1 Instructions...

  • Page 3: Introduction

    Introduction Thank you for purchasing the Nikon product. This instruction manual is written for the users of the Nikon Microscope ECLIPSE FN1. To ensure correct usage, please read this manual carefully before operating the product. • It is prohibited to reproduce or transmit this manual in part or whole without Nikon's expressed permission.

  • Page 4: Meaning Of The Symbol Used On The Product

    Caution for heat This symbol on the lamphouse of the Eclipse FN1 calls your attention on the following: • The lamp and its surroundings (including the lamp house) become very hot during and immediately after lighting.

  • Page 5: Safety Precautions

    Malfunctions and damage due to disassembling are unwarranted. Never attempt to disassemble any part other than the parts described in this manual. If you experience problems with the microscope or the microscope system, contact your nearest Nikon representative. Read the instructions carefully To ensure safety, carefully read this manual and the manuals provided with any other equipment used with this microscope.
  • Page 6 Because safety is a top priority in the design of Nikon products, the hazards described above should not pose any danger as long as you heed all of the warnings and cautions in the manuals and use the system only for its intended purpose.

  • Page 7: Caution

    Specified light source: Sumita Optical Glass Inc. Halogen light source LS-DWL-N • For the illumination with the lamp house Specified lamp house: Nikon FN-LH Specified lamp: 12V 100W Long life halogen lamp (PHILIPS 7724i or ODRAM HLX 64623) Specified power supply: Nikon TE2-PS100W * In addition, when the lamp house is used for the illumination, a lamp house adapter must be attached to connect the lamp house with the microscope FN1.
  • Page 8 Safety Precautions CAUTION Cautions in assembling, installing, and carrying the microscope system • Take care to avoid pinching your finger or hand during the microscope system assembly and installation. • Scratches or fouling such as fingerprints on optical components (lenses, filters, and so on) will degrade microscope images.

  • Page 9: Notes On Handling The Microscope System

    Notes on Handling the Microscope System Notes on Handling the Microscope System Handle with care This microscope system is a precision optical instrument. Handle the microscope system with care to avoid shock on impact. In particular, objectives may loose accuracy when exposed to even a weak physical shock. Weak electromagnetic waves The power supply for this microscope system emits weak electromagnetic waves.
  • Page 10 Do not apply any pressure onto the blades to prevent malfunctions. 10 Contents of the package The contents of the package are listed below. Check them. • Microscope ECLIPSE FN1 Main body Substage part Eyepiece tube mount part Nosepiece arm part...
  • Page 11 Notes on Handling the Microscope System Two clamp screws for the eyepiece tube mount part front side (long type) Two clamp screws for the eyepiece tube mount part rear side (short type) Sixteen clamp screws for base auxiliary legs Sixteen washers for base auxiliary legs •...

  • Page 12: Table Of Contents

    Contents Contents Introduction............................1 WARNING and CAUTION Symbols Used in This Manual ........... 1 Meaning of the symbol used on the product ............... 2 Safety Precautions........................... 3 WARNING.......................... 3 CAUTION........................... 5 Notes on Handling the Microscope System ................ 7 Chapter 1 Part Name ........................
  • Page 13 Contents Polarizer Turret ....................32 11.1 Attaching Filters ....................33 11.2 For the DIC microscopy ................... 33 11.3 Contrast Adjustment..................35 Chapter 4 Assembly........................36 Chapter 5 Troubleshooting ......................49 Viewing Problems and Control Problems ............49 Electrical System Problems................. 52 Chapter 6 Care and Maintenance ....................

  • Page 14: Chapter 1 Part Name

    Condenser centering screw Base auxiliary leg This photo depicts the ECLIPSE FN1 microscope configured with the epi-fl attachment, the variable magnification double port attachment, and the ergonomic eyepiece tube. * The product name for the variable magnification double port attachment is "Mag. Variable Double Port."...
  • Page 15 Chapter 1 Part Name Focus adjustment ring for the rear port Analyzer selector lever Lamp house Parfocal correction Objective knob Coarse focus torque adjustment ring Stage movement knob Rear side coarse focus knob for the Y-axis Rear side fine focus knob Stage movement knob for the X-axis Front side coarse focus knob...

  • Page 16: Chapter 2 Microscopy

    Microscopy The general procedure for the microscopy with water immersion objective is described below. For details, refer to Chapter 3, "Operation of Each Part." Turn on the power supply or the optical fiber light source. (Push the switch to the "I" postion.) Adjust the brightness with the brightness control knob on the power supply or on the optical fiber light source.
  • Page 17 Chapter 2 Microscopy When the trinocular eyepiece tube is attached, push in the optical path selector lever and select the optical path to make 100% light go into the binocular part. Refer to Section 4.1 in Chapter 3, "Selecting the Optical Path in the Eyepiece Tube (T Eyepiece Push in Tube, F Eyepiece Tube)."...
  • Page 18 Chapter 2 Microscopy Move the objective vertical movement lever to the left so that the objective tip touches the surface of the water over the specimen. To perform the parfocal correction, use the parfocal correction knob on the sliding nosepiece. With this focusing operation, a confocal position for the two objectives can be attained.

  • Page 19: Chapter 3 Operation Of Each Part

    Operation of Each Part Coarse Focus Knob and Fine Focus Knob Focus Knob Rotation and the Objective Vertical Movement The rear side fine focus knob is marked in 1 µ m increments. The objective rises/falls approximately 0.1 mm per one turn of the fine focus knob.

  • Page 20: Sliding Nosepiece

    Chapter 3 Operation of Each Part Sliding Nosepiece Sliding Nosepiece The sliding nosepiece can be attached two objectives into the near side socket and the far side socket. The objectives are changed by sliding the nosepiece to and from. Sliding nosepiece Objective Vertical Movement Lever and the Objective Position You can move the objective upward and downward by Objective vertical...

  • Page 21: Switching Objectives

    Chapter 3 Operation of Each Part Sliding Nosepiece Switching Objectives In this section, an example case is explained. (Changing objectives from the near side to the far side) CAUTION: You cannot change objectives when the objective vertical movement lever is placed at the right or the left.

  • Page 22: Parfocal Adjustment For The Objectives

    Chapter 3 Operation of Each Part Sliding Nosepiece Parfocal Adjustment for the Objectives Usually, the focal length for a dry type objective is different Parfocal correction knob from that of a liquid immersion type objective. If you change objectives between these types with no correction, the image will go out of focus.

  • Page 23: Attaching/Detaching The Dic Slider

    Chapter 3 Operation of Each Part Single Objective Holder Attaching/Detaching the DIC Slider To attach/detach the DIC slider for the DIC microscopy, lower the objective. The DIC sliders are inserted into the DIC slider slots on the front right side of the both objectives. DIC slider Single Objective Holder Single objective holder...

  • Page 24: Diopter Adjustment

    Chapter 3 Operation of Each Part Eyepiece Tube Vertical tube adapter A photo adapter is attached as a standard accessory for photomicrographic equipment. To use this adapter, insert it into the vertical tube of the eyepiece tube and fix it with three screws on the vertical tube.

  • Page 25: Interpupillary Distance Adjustment

    Chapter 3 Operation of Each Part LWD Condenser Observe the specimen with both eyes and focus on the specimen surface by using the focus knob. Rotate the right diopter adjustment ring while observing the specimen with your right eye so that the reticle and the specimen come into focus.

  • Page 26: Adjusting The Condenser Aperture Diaphragm

    Chapter 3 Operation of Each Part LWD Condenser Place an objective of 40x or 80x into the optical Parfocal path. Rotate the parfocal correction knob on the correction sliding nosepiece to focus on the specimen. knobs Near side Far side objective objective Turn the condenser focus knob to form the field diaphragm image on the specimen surface.

  • Page 27: Attaching An Optical Element Into The Lwd Condenser Turret

    Chapter 3 Operation of Each Part LWD Condenser Aperture diaphragm adjustment with the pupil of the objective Remove one of the eyepieces and look into the sleeve. Pupil of the objective You can see the pupil of the objective (that is a bright area when the aperture diaphragm is fully opened.) Stop down the condenser aperture diaphragm so that the diaphragm 70~ 80...

  • Page 28: Operation Of The Lwd Condenser For Each Microscopy

    Chapter 3 Operation of Each Part LWD Condenser Operation of the LWD Condenser for Each Microscopy For the bright-field microscopy Place the vacant hole of the turret into the optical path. For the bright-field microscopy with the 4x objective Attach the additional lens for 4x magnification into the DIC prism N2 position of the condenser turret of the LWD condenser and fix it with a set-screw.

  • Page 29: Field Diaphragm

    Chapter 3 Operation of Each Part Field Diaphragm Field Diaphragm The field diaphragm restricts the illumination light to the area on the specimen to be observed. Operating the field diaphragm ring changes the size of the field diaphragm. For normal observation, the size of the diaphragm should be such that it is just outside the edge of the viewfield.

  • Page 30: Water Immersion Operation (Water Immersion Type Objective Handling)

    Chapter 3 Operation of Each Part Water Immersion Operation (Water Immersion Type Objective Handling) Water Immersion Operation (Water Immersion Type Objective Handling) An objective with a "W" mark is a water immersion type objective. It is designed to focus on a specimen at a working distance in water.

  • Page 31: Variable Magnification Double Port Attachment

    Chapter 3 Operation of Each Part Variable Magnification Double Port Attachment Variable Magnification Double Port Attachment Front port You can attach an eyepiece tube onto the front port of the Rear port variable magnification double port attachment and a 2/3 inch TV camera onto the rear port of the variable magnification double port attachment.

  • Page 32: Port Selection

    Chapter 3 Operation of Each Part Variable Magnification Double Port Attachment Port Selection 10.1 To use the front port and the rear port simultaneously, Rotate the port selector knob 180 degree in a counterclockwise direction. To use the front port only, Rotate the port selector knob 180 degree in a clockwise direction.

  • Page 33: Aligning The Optical Paths For The Front Port And The Rear Port

    Chapter 3 Operation of Each Part Variable Magnification Double Port Attachment Aligning the Optical Paths for the Front Port and the Rear Port 10.4 To align the optical paths for the front port and the rear Set screws port, adjust the rear port position. Rotate the two M4 set screws to adjust the rear port position (for the 2/3 inch TV camera).

  • Page 34: For The Dic Microscopy

    Chapter 3 Operation of Each Part Polarizer Turret For the DIC Microscopy 10.7 To perform the infrared DIC microscopy through the rear port Push in the lever on the left side of the variable magnification double port attachment to locate the IR analyzer into the optical path.

  • Page 35: Attaching Filters

    Chapter 3 Operation of Each Part Polarizer Turret Attaching Filters 11.1 The polarizer turret consists of the upper turret and the NCB filter lower turret. In the standard configuration, three types of ND filters are attached (ND2, ND4, and ND16) in the lower turret. One position of the turret is an empty hole position.
  • Page 36 Chapter 3 Operation of Each Part Polarizer Turret Attaching the IR Polarizer Upper part Separate the upper part (band pass filter and 1/4 (Band pass filter and 1/4 lambda plate) lambda plate) and the lower part of the IR polarizer. Lower part Insert the adjustment tool for the 1/4 lambda plate Adjustment...

  • Page 37: Contrast Adjustment

    Chapter 3 Operation of Each Part Polarizer Turret Remove the 1/4 lambda plate and insert the upper part of the IR polarizer (the band pass filter and the 1/4 lambda plate). Contrast Adjustment 11.3 Insert the DIC slider into the single objective holder or into the sliding nosepiece to locate the DIC prism of the condenser into the optical path.

  • Page 38: Chapter 4 Assembly

    Assembly WARNING To prevent electrical shock or fire, turn off the power switch of the power supply (flip the switch to the "O" side) during assembly. The electrical power should be supplied after assembling the microscope system. CAUTION • When assembling the microscope, assemble the eyepiece tube first. •...
  • Page 39 Chapter 4 Assembly Configuration diagram C-mount TV camera C-mount TV adapter TV tube C-TEP DSC Port Ergonomic Trinocular Tube Trinocular Tube Eyepiece Tube ① ② ③ ③ ③ ③ ③ Simple Polarizing DIC/IR Mag. Variable Mag. Variable Double Port Attachment Analyzer Turret ①...
  • Page 40 (for at least 30 minutes after the lamp is turned off), before replacing lamps. • Use the Nikon FN-LH model for the lamp house. • To attach the FN-LH lamp house onto the FN1 microscope body, the FN-LPA lamp house adapter (optional) is required.
  • Page 41 Chapter 4 Assembly Separate the vertical tube part, which includes a lens, and the lamp house connector part of the lamp house adapter. Insert the vertical tube part only in place of the optical fiber adapter. And then, fix the vertical tube part with three M3 hexagonal socket head bolts.
  • Page 42 Chapter 4 Assembly While pressing the lamp clamp lever, push Lamp socket the lamp into the socket pin holes as far as it will go. (Don't touch the glass surface of the lamp with bare hands.) Return the lamp clamp lever to its original position. Make sure that the lamp is not tilted. Attach the lamp house cover securely and tighten the clamp screw.
  • Page 43 Chapter 4 Assembly Installing the Epi-fl attachment/variable magnification double port attachment/simple DIC analyzer Epi-fl attachment (1) Remove the ultraviolet light shield and the button bolts at the mounting part. (2) Fix epi-fl attachment eyepiece tube mount part microscope main body with an M4 hexagonal socket head set screw.
  • Page 44 Chapter 4 Assembly Attaching an eyepiece tube M4 hexagonal Attach an eyepiece tube on the eyepiece tube socket head mount part of the microscope main body and fix it set screw with an M4 hexagonal socket head set screw. When using an epi-fl attachment, the variable magnification double port attachment, or the simple DIC analyzer, attach the eyepiece tube onto the eyepiece tube mount part of the upper part of the...
  • Page 45 Chapter 4 Assembly Attaching the auxiliary stage support Mounting hole for the auxiliary stage support column columns Move the stage left and right and around so that you can access the mounting hole for the auxiliary stage support column. Hold an auxiliary stage support column and bring it to the stage from below.
  • Page 46 Chapter 4 Assembly Attaching the LWD condenser Rotate the condenser focus knob on the substage to lower the condenser holder to the lowermost position. And then, insert the LWD condenser into the condenser holder. Insert Condenser focus knob Adjust the LWD condenser so that the Clamp screw aperture diaphragm lever comes to the front.
  • Page 47 Chapter 4 Assembly Attaching the polarizer turret Insert the polarizer turret into the receptacle step on the microscope base. Tighten the clamp screw when the polarizer turret reaches the limit. Clamp screw Attaching objectives For the sliding nosepiece Rotate the coarse focus knob to move the nosepiece arm to the upper limit. Move the objective vertical movement lever on the sliding nosepiece to the center.
  • Page 48 Chapter 4 Assembly Attaching the DIC module Attach the DIC module into the turret of the LWD condenser. Cover Loosen the hexagonal socket head screw at the upper part of the LWD condenser with the hexagonal screwdriver. And then, remove the cover. You can see an opening for the DIC module and the diagonal illumination diaphragm inside the cover.
  • Page 49 Chapter 4 Assembly Attaching the FN-MS Magnet Scale Rotate the coarse focus knob to raise the nosepiece arm to the upper limit. Loosen four screws using a Phillips screwdriver to remove the back cover of the microscope body at the rear side of the arm. Fix the Magnet Scale into the place with two Do not place the detector part M3 screws.
  • Page 50 Chapter 4 Assembly Attaching cables to reduce noise There are screw holes on the microscope body and on the accessories to attach cables to reduce noise. Microscope body Eyepiece tube mount part: one M3 screw hole on the right side Mainstay: one M4 hexagonal socket head bolt on the right side Lower part of the body: one M3 screw hole on the right side Sliding nosepiece: two M3 hexagonal socket head bolts on the both sides...

  • Page 51: Chapter 5 Troubleshooting

    Troubleshooting Improper use of the microscope may adversely affect performance, even if the microscope is not damaged. If any of the problems listed in the table below arise, take the countermeasures indicated. Viewing Problems and Control Problems Problem Cause Countermeasure The optical path selector lever on the eyepiece tube is in an intermediate position.
  • Page 52 Chapter 5 Troubleshooting Viewing Problems and Control Problems Problem Cause Countermeasure The aperture diaphragm is Open the diaphragm to a suitable size. stopped down too far. Adjust the condenser focus knob so that the The condenser position is too low. field diaphragm image is focused on the specimen surface.
  • Page 53 Chapter 5 Troubleshooting Viewing Problems and Control Problems Problem Cause Countermeasure Adjust the brightness with the brightness The lamp voltage is too low. control knob on the power supply. The brightness is insufficient. The aperture diaphragm is Open the diaphragm to a suitable size. stopped down too far.

  • Page 54: Electrical System Problems

    Chapter 5 Troubleshooting Electrical System Problems Electrical System Problems Problem Cause Countermeasure The power cord is not connected Plug the power cord into a wall outlet. to a wall outlet. The power cord is not connected Connect the power cord to the AC inlet on the to the AC inlet on the power power supply.

  • Page 55: Care And Maintenance

    Before putting on the dust-proof cover, turn off the power switch of the power supply (flip it to the "O" position) and wait until the lamp house gets cool sufficiently Regular inspection Regular inspection of this microscope system is recommended to maintain peak performance. Contact your nearest Nikon representative for details.

  • Page 56: Chapter 7 Specifications

    Specifications ■ Nikon microscope ECLIPSE FN1 Model name ECLIPSE FN1 Main body Optical system CFI60 and CFI75 optical system (infinity-corrected CF optical system) Main body "I" shape body, external power supply system Focusing mechanism Nosepiece up/down mechanism Manual operation type single axis coarse/fine focus knob mechanism (coarse/fine...
  • Page 57 • This is UL-listed product. (UL61010A-1) Safety standard • This product meets FCC Part 15B Class A requirements. Eclipse FN1 and the following equipment This equipment has been tested and found to comply with the limits for a were tested for the UL Class A digital device, pursuant to Part 15 of the FCC Rules.
  • Page 58 Chapter 7 Specifications ■ Outer dimensions 551.2 185.5 1(coverslip)
  • Page 59 Chapter 7 Specifications 486.7 185.5 1(coverslip) 151.4...

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