Nikon Eclipse LV100DA-U Instructions Manual page 102

Problem
Dirt or dust is seen in
the viewfield.
Diascopic
microscopy
The viewing is poor (too
much or too little
contrast, or poor
resolution).
Epi-fl
microscopy
Diascopic
microscopy
The focus is uneven.
The image is elongated.
Or, the image shifts
during focus.
Diascopic
microscopy
100
Cause
The aperture diaphragm is stopped
down too far.
Dirt or dust exists on the lens, eyepiece,
filter, or specimen.
The upper surface of the condenser is
not clean.
The condenser position is too low.
Dirt or dust exists on the lens, eyepiece,
filter, or specimen.
The used objective is not suitable for
the microscopy.
The aperture diaphragm is stopped
down too far.
The filter cube being used is not suited
for the specimen.
There is no cover glass in place.
The glass slide is fluorescing.
The condenser position is too low.
The nosepiece is not attached correctly.
The nosepiece is not placed to a click-
stop position. (The objective is not
placed in the optical path).
The specimen holder is slanted.
The nosepiece is not attached correctly.
The nosepiece is not placed to a click-
stop position.
The stage is tilting.
The microscope is not installed on a flat
surface.
The condenser has not been centered.
Countermeasure
Open the diaphragm to a suitable size.
(p. 51 and 54)
Clean the components.
Clean the components.
Adjust the condenser focus knob so that
the field diaphragm image is focused on
the specimen surface.
Clean the components.
Use the designated objective.
(p.38, 39, 43, and 93)
Open the diaphragm to a suitable size.
(p. 51 and 54)
Use a filter cube suited for the
specimen. (p. 38, 68 to 70, and 84)
Use a cover glass.
(However, no cover glass is required
when using an NCG objective.)
Use a nonfluorescent glass slide.
Adjust the condenser focus knob so that
the field diaphragm image is focused on
the specimen surface.
Install the nosepiece correctly.
Push the OBJ. switch several times to
move the objective into the optical path.
Attach the specimen holder correctly.
Install the nosepiece correctly.
Push the OBJ. switch several times to
move the objective into the optical path.
Attach the stage correctly.
Install the microscope on a flat and
level surface.
Center the condenser.
(p. 107)
(p. 107)
(p. 53)
(p. 107)
(p. 53)
(p. 79)
(p. 43)
(p. 77)
(p. 79)
(p. 43)
(p. 76)
(p. 53)