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5
Differential Interference Contrast Microscopy under the Epi Illumination (Prism Slide Method)
Methods of the differential interference contrast (DIC) microscopy vary among the nosepiece
configuration. For motorized universal quintuple nosepieces, the LV-NU5A and the LV-NU5AC,
the prism slide method is used for the DIC microscopy under the epi illumination.
Attach the accessories required for the prism slide method of the DIC microscopy
1
under the episcopic illumination.
The accessories required for the prism slide method of the DIC microscopy under the episcopic
illumination are as follows:
•
Motorized universal quintuple nosepiece (LV-NU5A or LV-NU5AC) (See Page 79.)
•
LU objective (See Page 93.)
•
Polarizer slider (for the episcopic illumination) (See Page 83.)
•
Analyzer slider (See Page 83.)
•
Lambda plate slider (for the sensitive color microscopy) (See Page 81.)
•
DIC slider (L-DIC/L-DIHC, attached to the DIN slot of the nosepiece) (See Page 83.)
*
Set up the microscope according to the "LVSetup" and set information of objectives correctly.
Focus on the specimen with the bright-field microscopy under the episcopic
2
illumination.
(See Pages 15 and 16.)
Set the microscope for the DIC microscopy under the episcopic illumination.
3
1 Push in the analyzer slider to place the analyzer
into the optical path. (See Page 60.)
2 Push in the polarizer slider to place the polarizer
into the optical path, and set the crossed Nicols
position by aligning the index. (See Page 57.)
* The crossed Nicols position can be set by placing
the PA block (LV-PAB) into the optical path
instead of the analyzer and the polarizer.
(See Page 61.)
3 Press the OBJ. switch on the operation panel to
place the objective of a desired magnification into
the optical path. (See Page 43.)
The aperture diaphragm for the episcopic
illumination is adjusted to the predetermined size
by the interlock control function.
4 Push in the DIC slider to place the DIC prism into
the optical path. (See Page 65.)
22
Set the polarizer to the
crossed Nicols position.
1
2 5
F. ST OP
IN
OUT
BF
DF FL1 FL2
FL1
FL2
4 5 6
3
0.8
0.7
Achr N.A
0.6
= 0.9
0.5 0.4
0.3 0.2 0.1
JAPAN
Operation
panel
ND8
NCB
F.S.
7
3
OBJ.switch
EPI
DIA
A.S.
OBJ.
CUBE
8
UEP I2A
USB
RS2 32C
LCN T
EPI brightness switch
EPI
DIA
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