Polarization Microscopy Under The Dia Illumination (Simplified/Sensitive Color) - Nikon Eclipse LV100DA-U Instructions Manual

8

Polarization Microscopy under the Dia Illumination (Simplified/Sensitive Color)

Attach the accessories required for the polarization microscopy under the dia illumination.
1
The following accessories must be attached to perform the simplified/sensitive color polarization
microscopy under the dia illumination.
• Diascopic slider (See Page 94.)
• Analyzer slider (See Page 83.)
• Lambda plate holder (for the sensitive color microscopy) (See Page 80.)
Focus on the specimen with the bright-field microscopy under the diascopic
2
illumination.
(See Pages 26, to 27.)
Set the microscope for the polarization
3
microscopy under the diascopic illumination.
1 Push in the analyzer slider to locate the analyzer
into the optical path. (See Page 60.)
2 Locate the polarizer for the diascopic
illumination and make a crossed Nicols position.
(See Pages 58 and 59.)
Swing-out
center
3 To perform the sensitive color polarization
microscopy, push in the lambda plate slider to
locate the lambda plate into the optical path.
(See Page 63.)
4 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
5 Adjust the brightness with the ND filter.
(See Page 44.)
Return to the bright-field microscopy under the
4
diascopic illumination.
1 Pull out the analyzer slider and move the analyzer
away from the optical path. (See Page 60.)
2 Move the diascopic polarizer away from the
optical path. (See Pages 58 and 59.)
3 Pull out the lambda plate slider and move the
lambda plate away from the optical path.
(See Page 63.)
4 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
5 Adjust the brightness with ND filters. (See Page 44.)
28
Set the polarizer to the
crossed Nicols position.
1
F. ST OP
BF DF FL1 FL2
FL1 FL2
3
0.8
0.7 Achr N.A
0.6 = 0.9
0.5 0.4 JAPAN
0.3 0.2 0.1
2
Operation
panel
ND8 NCB
F.S.
EPI
DIA
A.S.
OBJ.
CUBE
1
F. ST OP
BF DF FL1 FL2
FL1 FL2
3
0.8
0.7 Achr N.A
0.6 0.5 0.4 = 0.9 JAPAN
0.3 0.2 0.1
2
Operation
panel
NCB
ND8
F.S.
EPI
DIA
A.S.
OBJ.
CUBE
UEP I2A
USB
RS2 32C
LCN T
5
4
EPI
DIA
brightness
DIA
switch
UEP I2A
USB
RS2 32C
LCN T
5
4
EPI
DIA
brightness
DIA
switch