Nikon Eclipse LV100DA-U Instructions Manual page 69

Combination of the DIC prisms for the DIC microscopy under the diascopic
illumination and the objectives
The combination of the DIC prisms differs depending on the used objective. Be careful that the DIC
contrast images cannot be obtained or the contrast is reduced excessively if the combination is
wrong.
To obtain the images with the higher contrast or resolution for the observation purpose, use the
special prism. However, the contrast and the resolution are contradictory in principle. (When the
contrast is increased, the resolution is reduced.) Select the combination suitable for the purpose.
Combination of the DIC prisms for the DIC microscopy under
Objective
10X Plan Apo 10XA
Plan Fluor 10X
S Fluor 10X
Fluor 10X W
20X Plan Fluor 20X
Plan Fluor 20X MI
Plan Apo 20X
S Fluor 20X
Fluor 20X W
40X Plan Fluor 40X
Plan Apo 40X
S Fluor 40X
Plan NCG 40X
Plan Fluor 40X Oil
Fluor 40X Oil
Fluor 40X W
Plan Apo 40X Oil
60X Plan Apo 60X Oil A
Plan Apo 60X
Fluor 60X W
Plan Apo VC 60X H
CFI APO TIRF 60X Oil
Plan Fluor 60X Oil
Plan Apo TIRF 60X Oil
Plan Apo 60X WI
Plan Apo VC 60X WI
Plan Fluor 60X A
100X Plan Apo VC 100X H
CFI APO TIRF
100X Oil
Plan Apo 100X NCG Oil
Plan Fluor 100X Oil
Plan Fluor 100X Oil, iris
Plan Apo 100X Oil
Plan Apo TIRF 100X
Plan Apo 100X NCG Oil
the diascopic illumination and the objectives
Standard
Objective Condenser
DIC prism DIC prism
10X N1 Dry
20X
40X I
40X II
N2 Dry
60X I
60X II
100X I
100X II
III. Operation of Each Part
Contrast priority
Objective Condenser
DIC prism DIC prism
20X-C
N1 Dry
40X I-C
Resolution priority
Objective Condenser
DIC prism DIC prism
60X I-R
60X II-R
NR Dry
100X I-R
100X II-R
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