Bright-Field Microscopy - Nikon eclipse lv150 Instruction Manual

1

Bright-Field Microscopy

When configured with the LV-UEPI
1. Turn on the power.
2. Set the microscope for
bright-field microscopy
If accessories for DIC
microscopy (*1 to *3)
are in place, pull them
out of the optical path.
3. Place the sample on the
stage and focus on it.
(p.27)
4. Adjust the diopter. (p.30)
5. Adjust the interpupillary
distance. (p.30)
6. Change the magnification
and observe the sample.
Hint:
It may be difficult to
focus on a sample with
small contrast, such on a
polished surface. In a
case like this, stop down
the field diaphragm so
that its image can be
seen in the viewfield,
and try to focus on the
rim of the diaphragm
image. When the rim is
in focus, the sample is in
focus just as well.
16
Push in.
1
Binocular eyepiece: 100% (P.29)
*1
Push in.
2
BF (bright-field) (p.33)
Select the
*2
10x objective.
3
On the LV150A,
use the nosepiece
rotation buttons. (p.35)
Lower the stage
4 as far as it will go.
Coarse focus knob
(p.27)
Adjust the
brightness.
5
Brightness control
knob (p.26)
Select the
1
10x objective.
On the LV150A,
use the nosepiece
rotation buttons.
(p.35)
Finely adjust
2
the focus.
Coarse/fine focus
knob (p.27)
Adjust the
3 brightness.
Brightness control
knob (p.26)
To fully open the field and
OUT
IN 100
0
0
100
A ST O P
P
F. ST O
B F
D F
*3
Power switch
Adjust to circumscribe
Field diaphragm (p.31)
Image of field diaphragm
Adjust to 70 to 80% of
the objective's N.A.
OUT
IN
100
0
0
100
P
A. ST O
P
F. ST O
B F
D F
Raise the levers.
6
aperture diaphragms.
(p.31 and p.32)
Push in the
NCB11 filter. 7
To compensate
color temperature.
(p.26)
Adjust the
brightness.
8
ND filter
(p.26)
the viewfield.
4
Viewfield
5
Aperture diaphragm
(p.32)
Objective's
pupil
Image of
aperture
diaphragm
Adjust
the brightness.
6
ND filter
(p.26)