Nikon Eclipse LV100DA-U Instructions Manual page 104

Problem
The coarse focus knob
is heavy in rotation.
The stage falls on its
own weight and the
image goes out of focus.
The stage cannot be raised
by the coarse focus knob.
No interference color is
seen in the DIC
microscopy.
Diascopic
microscopy
Uneven colors are seen
or low contrast image is
seen in the DIC
microscopy.
Diascopic
microscopy
No sensitive color is seen in
the polarization microscopy.
Diascopic
The
contrast of microscopy
the phase
contrast
microscopy
is poor.
102
Cause
The coarse torque adjustment ring is
tightened too much.
The coarse focus stopper ring is locked
to restrict the upper limit.
The coarse torque adjustment ring is
loosened too much.
The coarse focus stopper ring is locked
at the lower limit.
No polarizer is placed in the optical path.
No analyzer is placed in the optical path.
The analyzer and the polarizer are not
at the crossed Nicols position.
No objective DIC prism is placed in the
optical path.
The combination of the objective and
the objective DIC prism is wrong.
The condenser DIC prism is not placed
in the optical path.
The combination of the objective and
the condenser DIC prism is wrong.
A wrong type of objective is used.
The orientation of the polarizer is
wrong.
Dust exists on the objective, the
condenser, or the specimen.
The combination of the objective and
the objective DIC prism is wrong.
A wrong condenser DIC prism is
selected.
The field diaphragm image is not
focused on the specimen surface.
No lambda plate is placed in the optical
path.
The Ph annular diaphragm of the
condenser does not coincide with the
phase plate ring of the objective.
The Ph annular diaphragm of the condenser is
not suitable for the Ph code of the objective.
The field diaphragm image is not
focused on the specimen surface.
Countermeasure
Loosen the torque adjustment ring
adequately.
Turn the coarse focus stopper ring to
release the stopper function.
Tighten the torque adjustment ring
adequately.
Turn the coarse focus stopper ring to
release the stopper function.
Place it into the optical path.(p.57 to 59, and 61)
Place it into the optical path. (p.60 and 61)
Adjust the orientation of the polarizer to
make the crossed Nicols position.
Place it into the optical path.
Place the DIC prism suitable for the
objective into the optical path. (p. 64 and 66)
Place the condenser DIC prism into the
optical path.
Place the DIC prism suitable for the objective
into the optical path.
Use an objective suitable for the DIC
microscopy. (p. 64 to 66, and 93)
Adjust the orientation of the polarizer
correctly.
Clean it. (Pay great attention to dust for
the differential interference contrast
microscopy.)
Place the DIC prism suitable for the
objective into the optical path. (p.64 to 66)
Place the DIC prism suitable for the objective
into the optical path.
Focus the condenser.
Plase it into the optical path
(p. 62 and 63)
Center the Ph annular diaphragm until it
coincides with the phase plate ring. (Refer
to the instruction manual for the LV-CUD.)
Place the Ph annular diaphragm specified
by the Ph code of the objective.
Focus the condenser.
(p. 46)
(p. 47)
(p. 46)
(p. 47)
(p. 64 to 66)
(p. 66)
(p. 66 and 67)
(p.57 and 59)
(p.107)
(p. 66 and 67)
(p. 53)
(p. 55)
(p. 53)