Dark-Field Microscopy Under The Dia Illumination; Dark-Field Microscopy Under The Dia Illumination - Nikon Eclipse LV100DA-U Instructions Manual

9

Dark-field Microscopy under the Dia Illumination

Attach the accessories required for the dark-field microscopy under the dia illumination.
1
The following accessories must be attached to perform the simplified/sensitive color dark-field
microscopy under the dia illumination.
• Universal condenser (dry) and dark-field annular diaphragm (See Page 78 and the LV-CUD
instruction manual.), or dark-field condenser (See Page 78.)
Focus on the specimen with the bright-field microscopy under the diascopic illumination.
2
(See Pages 26 to 27.)
Set the microscope for the dark-field
3
microscopy under the diascopic illumination.
1 Rotate the condenser turret of the universal
condenser to the "DF" position. (See Page 55.)
2 Press the OBJ. switch on the operation panel and
locate the desired objective into the optical path.
(See Page 43.)
The objective of a magnification of 4x or more
and a numerical aperture of 0.7 or less is usable
to the dark-field microscopy.
3 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
4 Adjust the brightness with ND filters.
(See Page 44.)
5 Fully open the field diaphragm and the aperture
diaphragm. (See Page 54.)
Return to the bright-field microscopy under the
4
diascopic illumination.
1 Rotate the condenser turret of the universal
condenser to the "O" position. (See Page 55.)
2 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
3 Adjust the brightness with ND filters.
(See Page 44.)
4 Adjust the field diaphragm and the aperture
diaphragm. (See Page 54.)
II. Microscopy Method
F . ST OP
BF DF FL1 FL2
FL1 FL2
2
1
5
NCB
Operation ND8
panel
F.S.
5
2
OBJ. switch
A.S.
OBJ.
CUBE
F . ST OP
BF DF FL1 FL2
FL1 FL2
1
4
NCB
ND8
Operation
panel
F.S.
4
A.S.
OBJ.
CUBE
UEP I2A
USB
RS2 32C
LCN T
4
EPI
EPI
DIA
DIA
3
DIA brightness switch
UEP I2A
USB
RS2 32C
LCN T
3
EPI
EPI
DIA
DIA
2
DIA brightness switch
29