Polarization Microscopy Under The Epi Illumination (Simplified/Sensitive Color) - Nikon Eclipse LV100DA-U Instructions Manual

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Polarization Microscopy under the Epi Illumination (Simplified/Sensitive Color)

Attach the accessories required for the polarization microscopy under the epi
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illumination.
The following accessories must be attached to the LV-UEPI2A to perform the polarization
microscopy under the epi illumination.
• Polarizer slider (See Page 83.)
• Analyzer slider (See Page 83.)
• Lambda plate slider (for the sensitive color microscopy) (See Page 83.)
* If only the simplified polarization microscopy is performed, the PA block (LV-PAB) can be used instead of the analyzer
and the polarizer. (See Page 84.)
Focus on the specimen with the bright-field microscopy under the epi illumination.
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(See Pages 15 and 16.)
Set the microscope for the polarization
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microscopy under the epi illumination.
1 Push in the analyzer slider to locate the analyzer
into the optical path. (See Page 60.)
2 Push in the polarizer slider to locate the polarizer
into the optical path, and get the crossed Nicols
position by aligning the index. (See Page 57.)
* For the simplified polarization microscopy,
you can get the crossed Nicols position by
placing the PA block (LV-PAB) into the
optical path instead of the analyzer and the
polarizer. (See Page 61.)
For the sensitive color polarization
microscopy, the lambda plate must be placed
between the analyzer and the polarizer. The
PA block (LV-PAB) cannot be used for the
sensitive color polarization microscopy.
3 For the sensitive microscopy, push in the lambda plate slider to locate the lambda plate into the
optical path. (See Page 62.)
4 Operate the EPI brightness switch on the operation panel to adjust the brightness of the episcopic
illumination. (See Page 42.)
5 Adjust the brightness with ND filters. (See Page 44.)
About sensitive color polarization microscopy under the epi illumination
Turn the polarizer rotation ring to adjust the polarization while observing the image.
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Set the polarizer to the
crossed Nicols position.
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2
F. ST OP
BF DF FL1 FL2
FL1 FL2
3
0.8
0.7 Achr N.A
0.6 = 0.9
0.5 0.4 0.3 0.2 0.1 JAPAN
Operation
panel
NCB
ND8
F.S.
A.S.
OBJ.
CUBE
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UEP I2A
USB
RS2 32C
LCN T
4 EPI brightness
switch
EPI
EPI
DIA
DIA