Episcopic Bright-Field Microscopy - Nikon ECLIPSE L200ND / ECLIPSE L200N Instructions Manual

2.1

Episcopic bright-field microscopy

1.
Set each part as follows.
You can perform steps in any sequence.
Lower the stage
to the limit.
Select 10 ×
objective.
Adjust brightness.
Press for EPI.
(For L200ND only)
2.
Place the sample on the stage.---------------------------------------------------
Move the stage to your right (or left) side.
3.
Focus on the sample. -----------------------------------
Use the focusing target for a sample with a polished surface.
4.
Adjust diopter.-----------------------------------------------------------------
5.
Adjust interpupillary distance. -----------------------------
6.
Switch to the desired objective and focus on the sample again.
7.
Adjust brightness. -------------------------------------------------------------------------
Use the ND filters and brightness control dial.
8.
Adjust the size of the aperture diaphragm. ---------------------------
2 Microscopy
Press in the NCB and ND filters.
(Insert the NCB filter into the
nearest slot to the lamphouse.)
Fully open the aperture diaphragm.
5
IC Inspection Microscope ECLIPSE L200ND / L200N Instructions
Press in for 100% binocular
observation.
Slide out the analyzer and
the polarizer.
Press in for BF (bright-field).
(For L200ND, press in to the
limit and then pull to the first
click-stop position for BF.)
Slide out the DIC slider.
Turn ON the power.
See "3.8 8 × 8 Stage"
See "3.8 8 × 8 Stage" and "3.9 Focusing target"
See "3.4 Diopter adjustment"
See "3.5 Interpupillary distance adjustment"
See "3.6 Aperture diaphragm"
See "3.1 Filters"