Dic Prism (For The Episcopic Illumination/Prism Slide Method) - Nikon Eclipse LV100DA-U Instructions Manual

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DIC Prism (For the Episcopic Illumination/Prism Slide Method)

When the DIC microscopy under the episcopic illumination is performed, applicable microscopy
method differs depending on the used nosepiece. When the motorized universal quintuple nosepiece
(LV-NU5A or LV-NU5AC) is used, the Prism Slide method is applicable.
* Use the objectives for the industrial microscope marked with "LU."
To perform the Prism Slide method of the DIC
microscopy under the episcopic illumination, set the
polarizer and the analyzer on the epi illuminator (LV-
UEPI2A) at the crossed Nicols position and insert the
DIC prism (DIC slider: L-DIC/L-DIHC*) into the
motorized universal quintuple nosepiece (LV-NU5A
or LV-NU5AC)
Attaching/removing the DIC slider
Fully loosen the DIC slider limiter screw on the
nosepiece with a hexagonal screwdriver. Insert the
DIC slider into the DIN slot on the nosepiece and
tighten the DIC slider limiter screw.
To remove the DIC slider from the nosepiece, fully
loosen the limiter screw with a hexagonal screwdriver
and pull out the DIC slider.
Placing the DIC prism into the optical path
Push in the slider to the second click-stop position to locate the DIC prism into the optical path. Pull
out the slider to the first click-stop position to remove the DIC prism from the optical path.
Setting the DIC prism
The corresponding position of the prism selector knob for each
objective is indicated on the objective body next to the
magnification and the N.A. value. On the objective shown in the
right figure, a letter "A" is indicated next to the magnification and
the N.A. value. It indicates that the corresponding DIC prism
position for this objective is "A." Thus, when you use this
objective, turn the prism selector knob on the DIC slider to match
the letter "A" with the white circle.
Interference color
You can change the interference color continuously by rotating the prism position knob located at
the tip of the DIC slider.
Interference color
Dark color
Gray
Sensitive red-violet
* L-DIHC: high contrast type
Observations similar to the dark-field microscopy can be performed.
You can observe the phase contrast distribution of the whole specimen with a bird's eye view.
Observations with the highest color contrast can be performed.
III. Operation of Each Part
Prism setting knob
Positioning groove
DIC slider (L-DIC/L-DIHC)
Limiter
screw
Characteristics
Prism position knob
CF Plan
10X/
0.30
A
/0 BD DIC
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