Nikon Eclipse LV100DA-U Instructions Manual page 61

Attaching the D-DP polarizer for the diascopic illumination and adjusting the
orientaion
Place the rotatable polarizer for the diascopic illumination
over the field lens at the microscope base with the polarizer
index facing the front and secure it with the clamp screw.
The orientation of the polarizer can be adjusted by loosening
the clamp screw on the side and rotating the upper part. The
orientation can be secured by tightening the clamp screw.
Normally, to perform the DIC microscopy, adjust the
orientation of the polarizer according to the following
procedure so that the palarizer and the analyzer are at the
crossed Nicols position when the orientation indicator is
located at the index.
1 Set the system for the bright-field microscopy for the diascopic illumination. Then, focus and
center the condenser.
2 Focus on the specimen.
3 Place the analyzer into the optical path.
4 Adjust the orientation of the rotatable polarizer for the diascopic illumination by loosening the
setcrew. And, tighten the setscrew with the index facing the front of the microscope.
5 Loosen the thumbscrew and rotate the upper part of the
rotatable polarizer for the diascopic illumination to align
the orientation indicator with the index.
6 Fully open the aperture diaphragm.
7 Remove one eyepiece from the eyepiece tube and look
inside the open sleeve. You can see the pupil of the objective
as a bright circle and can see black patterns in the circle.
8 Loosen the setscrew on the rotatable polarizer for the
diascopic illumination with the hexagonal screwdriver.
9 Rotate the rotatable polarizer for the diascopic
illumination until the dark cross is seen in the pupil of the
objective as shown in the right figure. The position that
the dark cross is seen is called the crossed Nicols.
Normally, the field of view at the crossed Nicols position
is the darkest.
10 Tighten the setscrew to secure the rotatable polarizer.
Swinging out the polarizer
The polarizer for the diascopic illumination can be
temporarily removed from the optical path by swinging out
the upper part of the polarizer. When the polarizer is not
needed for the microscopy, it can be removed easily with the
mechanism.
To place the polarizer into the optical path, reversely rotate
the upper part of the polarizer to the click-stop position.
III. Operation of Each Part
Rotatable polarizer for
the diascopic illumination
F.S .
D - D P
7 5 3 7 0 1
JAPAN
Thumbscrew
Swing-out
center
Setscrew
Orientation Index
indicator
Dark cross
Polarizer for the diascopic
illumination
Rotatable polarizer for the
diascopic illumination
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