Bright-Field Microscopy - Nikon ECLIPSE Si Instructions Manual

2.1

Bright-field Microscopy

Bright-field microscopy is the most basic observation method using diascopic illumination.
For details on the configuration and the assembly of the microscope for bright-field microscopy, see "4.2.1
Basic Configuration (For Bright-field Microscopy)."
1 Install the microscope.
(1) Carry the microscope from the storage location
and place it at your location.
(2) Fix the direction of the eyepiece tube to the
observation point (the binoculars are toward
you).
Loosen the fixing screw for the eyepiece tube a
couple of turns, and then turn the eyepiece tube.
Turn the eyepiece tube so that it faces the front, and
securely tighten the fixing screw for the eyepiece
tube.
(3) Pull out the AC adapter and power cord from the
storage part on the rear of the product.
2 Turn on the microscope.
Connect the AC adapter to power on the microscope.
(1) Confirm that the DC plug of the AC adapter is
connected to the DC input connector on the rear
of the main body.
(2) Plug the power cord of the AC adapter into an
electrical outlet
3 Lower the condenser from the upper limit.
When placing the specimen, lower the condenser
position by turning the condenser vertical movement
knob to avoid accidental contact with the condenser
lens.
4 Fully open the filed diaphragm and the aperture
diaphragm.
(1) Turn the field diaphragm dial clockwise until it
reaches the limit.
(2) Set the aperture diaphragm lever of the
condenser at the fully open position (leftmost).
Chapter 2 Microscopy Techniques
57
Face the eyepiece tube
Turn a
couple of
times to
loosen it.
Install the microscope.
Turn on the microscope.
Condenser vertical movement
Fully open the field diaphragm and
the aperture diaphragm.
Aperture diaphragm lever
• At the fully open position
Field diaphragm dial
• Turn it clockwise
until it reaches the
limit.