Differential Interference Contrast Microscopy Under The Dia Illumination - Nikon Eclipse LV100DA-U Instructions Manual

11

Differential Interference Contrast Microscopy under the Dia Illumination

The Senarmont method is used for the DIC microscopy under the dia illumination.
Attach the accessories required for the DIC microscopy under the dia illumination.
1
The following accessories must be attached to perform the DIC microscopy under the dia illumination.
• Universal condenser (dry) (See Page 78 and the LV-CUD instruction manual.)
• Rotatable polarizer for the diascopic illumination (See Page 94.)
• Analyzer (See Page 83.)
• DIC objective for biologic microscopes (See Page 93.)
• Condenser DIC prism (attached inside the universal condenser)
(See Page 66 and the LV-CUD instruction manual.)
• Objective DIC prism (attached to the nosepiece) (See Page 81.)
• Lambda plate holder (for the sensitive color microscopy) (See Page 80.)
* Set up the microscope according to the "LVSetup" and set information of objectives correctly.
Focus on the specimen with the bright-field microscopy under the diascopic illumination.
2
(See Pages 26 to 27.)
Set the microscope for the DIC microscopy under the diascopic illumination.
3
1 Push in the analyzer slider to locate the analyzer
into the optical path. (See Page 60.)
2 Attach the rotatable polarizer for the diascopic
illumination and rotate the upper part of the
polarizer to make a crossed Nicols position.
(See Page 59.)
3 Press the OBJ. switch on the operation panel and
locate the objective for the DIC microscopy into
the optical path. (See Page 43.)
4 Attach the objective DIC prism slider to the
nosepiece to locate the objective DIC prism into the
optical path. (See Page 66.)
5 Set the condenser turret to the "N1," "N2," or "NR"
position to locate the condenser DIC prism suitable
for the objective into the optical path. (See Page 55.)
6 Operate the DIA brightness switch on the
operation panel to adjust the brightness of the
diascopic illumination. (See Page 42.)
7 Adjust the brightness with ND filters. (See Page 44.)
32
D - D P
7 5 3 7 0 1
Thumbscrew JAPAN
Swing-out
center Setscrew
1
F . ST OP
BF DF FL1 FL2
FL1 FL2
4
3
0.8
0.7 0.6 Achr N.A
0.5 0.4 = 0.9 JAPAN
0.3 0.2 0.1
5
2 9
8
Operation ND8
panel
F.S.
8
3
OBJ. switch
A.S.
OBJ.
CUBE
UEP I2A
USB
RS2 32C
LCN T
NCB
7
EPI
EPI
DIA
DIA
6
DIA brightness switch