Chapter 3: Scanning Membranes; Placing The Membrane On The C-Digit Blot Scanner Scanning Surface - LI-COR C-DiGit User Manual

Chapter 3:
For Western blotting methods, nitrocellulose or PVDF membranes may be used. LI-COR offers
Odyssey nitrocellulose membranes (10 membranes; 0.22 μm, 7 cm x 8.5 cm) under part number
926-31090. There are some general tips for using membranes:
IMPORTANT: Do not touch the membrane – handle only with clean forceps. Lift the mem-
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brane only by the corners.
Keep the membrane wet if it is to be stripped and re-used. This can easily be accomplished by
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wrapping the membrane in plastic wrap.
Use clean containers to avoid cross-contamination and reduce background.
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Multiple membranes can be washed together, provided there is ample volume so each membrane
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moves freely.
Generally speaking, chemiluminescent detection protocols for Western blotting do not need to be
modified for use with the C-DiGit Blot Scanner. LI-COR does, however, have a variety of resources
available for optimizing Western blots. Some of the most common questions regarding imaging
with the C-DiGit Blot Scanner are listed below; more detailed support materials can be found at:
http://www.licor.com/bio/applications/chemiluminescent_western_blot/resources.html
Placing the Membrane on the C-DiGit Blot Scanner
Scanning Surface
The imaging surface is designed to scan a single membrane up to 10 x 8.5 cm. Place the membrane
face down, with the top of the membrane facing the back of the instrument.
Scanning Membranes
®
C-DiGit
Blot
Scanner
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