Table of Contents
Advertisement
Chapter 5: Maintenance & Troubleshooting
Image the plastic covering by itself first to determine if it scatters light, causing high background.
You may need to try several types of plastic coverings before finding the best one.
Wrapping the blot in plastic wrap may cause unwanted background, especially if it is folded or
handled roughly. When using plastic wrap it is important to avoid wrinkles, as they scatter light,
resulting in high background. In addition, try to avoid leaving fingerprints from pressing on the
blot.
Figure 5-2. Unwanted background caused by plastic wrap.
Low Signal
The C-DiGit Blot Scanner should be set to High Sensitivity to generate the highest signal.
Chemiluminescent substrates vary in quality and effectiveness, and using a substrate from a
different source may improve signal. Varying the primary and secondary antibody dilutions may also
affect the signal and background of a blot.
Troubleshooting –Image Studio™ Software
Problems
Q: What image resolution can I export?
A: Image Studio can export images at resolutions up to 600 dpi in TIF, JPG, and PNG format.
Q: Why doesn't my image import into Image Studio?
A: Image Studio supports TIFF, JPG and PNG files. These file formats come in numerous forms. If
your TIFF, JPG or PNG file does not import, please send the image to LI-COR so that future
updates will be compatible with your image file format.
Page 30
Advertisement
Table of Contents
Troubleshooting
