Lonza PyroTec PRO Hardware User Manual page 79

Cylindrical or cuboid-shaped cavities allow for optimal tracking. When using differently
shaped cavities, their inner dimensions and their immersion depth must be optimized.
The inner diameter of the cavities (or of test tubes) must be calibrated carefully and typed
in the corresponding data fields.
Fill Level of Cavities
To ensure safe handling (e.g transport by means of RoMa, PnP, etc.) of the containers,
make sure that the fill level of the cavities does not exceed the following limits:
• Fill the test tubes to a maximum of 80%.
Fill microplates to a maximum of 80% of the cavity volume
•
• Fill reagent troughs at maximum to the specified volume (e.g. 100 ml trough: 100 ml
equals approximately 90% of the total trough volume.
8.4.2.2 Liquid Handling with LiHa
Liquid Conductivity
When configuring samples that will be tested on PyroTec™ PRO, it may be necessary to
adjust conductivity settings such that the system is able to correctly detect liquid levels.
For more information surrounding the usage of different liquid classes, please contact
Lonza's Scientific Support.
Conductivity
Very good
Good
Bad
ATTENTION
Full system functionality can only be guaranteed when Lonza certified disposable tips are
used
ATTENTION
Contamination of the 3/2-way valve.
Make sure not to aspirate sample, or other liquid that may contaminate the valve, into the
3/2-way valve.
PyroTec™ PRO Hardw are User's Manual, V1.2
Liquid conductivity
Liquid
Serum, DNA solution, buffer
Tap water
DMSO, ethanol, distilled water
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Sensitivity
medium
high
very high
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RT-MN024-1.2 02/21