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Table of Contents
Related Manuals for Lonza Amaxa Nucleofector II
Summary of Contents for Lonza Amaxa Nucleofector II
- Page 1 Amaxa™ Nucleofector™ II Manual...
- Page 2 For more information and future program updates, check www.lonza.com/nucleofection-program-update Nucleofector™ II Chip Card For future protocol updates, also check www.lonza.com/cell-database Nucleofector™ II CD-ROM...
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Page 3: Table Of Contents
Contents The Amaxa™ Nucleofector™ Technology Operating Instructions Restrictions Maintenance Safety Instructions–Please Read Carefully Waste Disposal Nucleofector™ II Device Components Set-up Instructions Instructions for Using the Nucleofector™ II Device 2.7.1 Main menu and program execution 2.7.2 Selection of Nucleofector™ Programs by program code–“Free Program Choice” 2.7.3 Selection of Nucleofector™... - Page 4 Troubleshooting Nucleofector™ II Device Error Codes Appendix...
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Page 5: The Amaxa™ Nucleofector™ Technology
Constant Innovation The Nucleofector™ Technology offers you more opportunities than At Lonza, we believe that listening to our customers needs is the any other transfection method to approach your research topics best way to develop innovative products that exactly meet your from many different angles. - Page 6 Nucleofector™ Kits that contain cell- specific and optimized Nucleofector™ Solutions. The Nucleofector™ II Device with Kit Components As part of the Nucleofector™ Technology, Lonza provides cell-type specific Amaxa™ Optimized Protocols for many different cell lines and primary cells. An up-to-date database of the continuously growing range of Amaxa™...
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Page 7: Operating Instructions
Purchasers are granted a non-exclusive, non-transferable shielding or removal during extended UV exposure. license for a limited use of Lonza’s Nucleofector™ Technology and related software for research and development purposes, the The Nucleofector™ II Device is protected by two main fuses. Both... -
Page 8: Safety Instructions-Please Read Carefully
The Nucleofector™ II Device has been certified by international and Lonza certified cuvettes only. Use of any other solution safety standards and is safe to use when operated in accordance or cuvettes from any other source than Lonza will preclude all with this manual. warranty and liability claims. -
Page 9: Waste Disposal
Waste Disposal Rear side of Nucleofector™ II Device Disposal of consumables from Nucleofector™ Kits (cuvettes, pipettes and Nucleofector™ Solutions): Discard cuvettes, pipettes and expired Nucleofector™ Solution residuals in a biohazard Figure 2 container. Refer to your local waste management organization and to the relevant laboratory safety instructions for proper disposal practices. -
Page 10: Instructions For Using The Nucleofector™ Ii Device
Nucleofector™ II Device is now ready for program execution of programs for the transformation of bacteria (for details (see 2.7.5). please contact Lonza’s Scientific Support Teams (Europe/ World: +49 (0)221-991 99-400; USA: 800-521-0390) and ask 2.7.3 Selection of Nucleofector™ Programs by for the latest developments and experimental support). -
Page 11: Selection And Storage Of Nucleofector™ Custom Programs
2.7.4 Selection and storage of Nucleofector™ Custom Programs The Nucleofector™ II Software provides the option to add programs Saved custom programs can be selected by navigating to the Cell Type List by storing the appropriate program under a through the “Cell Type List” using the “Up” and “Down” buttons new individual name. -
Page 12: Additional Nucleofector™ Ii Settings And Options
Use of both uncertified cuvettes and re- and opening the cuvette holder carousel. In the menu settings you use of Lonza certified cuvettes (after even one use), impairs can choose between “on” for fully automated turning or “off” for experimental results, proper function of the device and risks manual opening and closing. -
Page 13: Language
In order to upgrade the program list on your Nucleofector™ II Device, insert the chipcard with the novel software version provided by Lonza into the chipcard reader slot (10) (Figure 7). After the Nucleofector™ II Device recognizes the chip card, confirm loading of the novel Nucleofector™... - Page 14 Phone +49 (0)221-99199-400 comments are intended to help optimize experimental conditions. Fax +49 (0)221-99199-499 scientific.support.eu@lonza.com Should you have any questions regarding the Nucleofector™ II www.lonza.com/nucleofection-support Device, please do not hesitate to contact Lonza’s Scientific Support Team. Phone 800-521-0390 Fax 301-845-8338 scientific.support@lonza.com www.lonza.com/nucleofection-support...
- Page 15 Problem Possible error What happened? Low survival rate Poor DNA quality. Plasmid DNA used should be of high purity. We strongly recommend the use of high quality products for plasmid purification such as QIAGEN Endo Free® Plasmid Kits. Do not use procedures involving phenol or chloroform treatment. Low gene transfer Plasmid amount is too low.
- Page 16 Should and is often accompanied by a flash and a noise. This problem these suggestions not resolve the problem, please call Lonza’s is usually caused by imperfect cuvettes or cuvette filling. The Scientific Support Team (Europe/World: +49 (0)221-99199-400;...
- Page 17 Code Possible error What happened? Procedure Err1A– Internal error or device possibly No pulse generated. Acknowledge the error message Internal communication failure defective. by pressing any button; try to apply program a second time. If Err1A re-occurs, switch off the device, wait for 2 seconds and switch on again.
- Page 18 Appendix Technical data Power supply 100–110 VAC or 230 VAC 50–60 Hz self-regulating Power consumption 50 VA/fuse T630mA L250V Temperature range +15°C to +40°C, non-condensing Altitude < 2000 m above sea level Safety class EN 61010-1 UL 61010A-1 IP 20 Weight 2.8 kg 6.2 lb...
- Page 19 Notes Appendix...
- Page 20 International Offi ces Lonza products only. Any use of the proprietary nucleic acid or protein other than as a positive control with a Lonza product is strictly prohibited. Use in any other Australia 61 3 9550 0883 application requires a license from Evrogen.