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Related Manuals for Lonza Amaxa HT Nucleofector
Summary of Contents for Lonza Amaxa HT Nucleofector
- Page 1 BioResearch Amaxa™ HT Nucleofector™ hardware manual For research use only...
- Page 2 The purchase of the HT Nucleofector™ conveys to the buyer the non-transferable right to use the system as well as Lonza’s proprietary Nucleofector™ technology only for research conducted by the buyer (whether the buyer is an academic or for-profit entity). For further details about the...
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Page 3: Table Of Contents
Content The Amaxa™ Nucleofector™ technology Operating instructions Restrictions Maintenance Safety instructions – read carefully Waste disposal HT Nucleofector™ system components System set-up instructions Instructions for using the HT Nucleofector™ system Running an experiment Processing a 384-well Nucleocuvette™ plate System shutdown Troubleshooting Nucleofection™... -
Page 4: The Amaxa™ Nucleofector™ Technology
With over 160 protocols for cell lines and primary cells optimized by reproducible easy-to-use technology that is suited to a wide variety of Lonza’s R&D team and more than 680 entries in the online cell data base, applications and provides an answer to almost every transfection Nucleofector™... - Page 5 HT Nucleofector™ system meets your screening requirements. Phone: + 49 221 99199 400 Experience the benefits of the HT Nucleofector™ system: E-mail: scientific.support.eu@lonza.com Automation-compatible – full integration into liquid handling platforms North America Fast – process 384 samples in one minute Phone: 800 521 0390 (toll free) Screening-compatible –...
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Page 6: Operating Instructions
Use a dry cloth to remove dust from the surface of the power supply. non-transferable right to use the system as well as Lonza’s proprietary NOTE: Do not use any liquid for cleaning the power supply. Liquids entering the unit may Nucleofection™... -
Page 7: Safety Instructions - Read Carefully
HT Nucleofector™ plate handler. Note: Lonza disclaims all warranties expressed or implied and shall in no event be liable – Safety may be compromised if any fluid has been spilled in the close for any kind of damages caused by or arising out of any operation or use in violation of the vicinity of the HT Nucleofector™... -
Page 8: Waste Disposal
HT Nucleofector™ software preinstalled on a Windows®-based laptop, and a set of cables to connect the units. Figure 2.1: HT Nucleofector system components 3. Power cable 4. Lonza interface data cable 5. Lonza interface HV cable 6. USB cable 1. HT Nucleofector™ power supply... - Page 9 It is connected to the plate handler plates that are loaded onto the carousel of the plate handler. via two cables. 13. Lonza interface (HV port) 7. Main switch with status LED 14. USB host (for firmware update) 8.
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Page 10: System Set-Up Instructions
Figure 2.6: Login screen – Connect the HV port of the HT Nucleofector™ power supply (10) to the HV port on the plate handler Unit (13) using the cable marked “Lonza interface HV cable.” – Connect the data port of the HT Nucleofector™ power supply (11) to the data port on the plate handler (17) using the cable marked “Lonza... -
Page 11: Instructions For Using The Ht Nucleofector™ System
2.7 Instructions for using the HT Nucleofector™ system The table below gives a brief overview of the steps required for a typical Nucleofection™ workflow. Step Description For more information see Switch on the HT Nucleofector™ system. This chapter Start the HT Nucleofector™ software on the computer and log in. Software manual Check your user settings (optional). -
Page 12: Running An Experiment
BioResearch HT Nucleofector™ hardware manual 3 Running an experiment This chapter explains how to run a Nucleofection™ experiment on the If the HT Nucleofector™ is integrated into an automated liquid handling HT Nucleofector™ system. Because correct cell handling before and system follow the appropriate instructions in the HT Nucleofector™... -
Page 13: Troubleshooting
HT Nucleofector™ system do not provide the expected results. The comments are intended to help optimize experimental conditions. Should you have any questions regarding the HT Nucleofector™ device, please do not hesitate to contact Lonza’s scientific support team Europe Phone: + 49 221 99199 400... -
Page 14: Mechanical Failures
2 (position 1 is visible), turn the carousel clockwise. 7. Remove the 384-well Nucleocuvette™ plate from the plate handler carousel. 8. Contact Lonza’s technical service for analyzing the error and for repair of the system. Figure 4.3: Turn carousel manually to recover samples... -
Page 15: Purchaser Notification
Lonza Cologne does not warrant either that the use of the license does not infringe third parties’ rights or does not cause damages to third parties. -
Page 16: Appendix
BioResearch HT Nucleofector™ hardware manual 6 Appendix Technical data power supply Power supply 100–110 VAC or 230 VAC 50–60 Hz Self-regulating Attention: Only use power outlets with protective earth/ground Only use the power cable supplied with the HT Nucleofector™ system Power consumption 750 VA / fuse T10A H250VP Operation temperature range... - Page 17 Notes...
- Page 18 BioResearch HT Nucleofector™ hardware manual Notes...
- Page 19 Notes...
- Page 20 Cologne GmbH. scientific.support@lonza.com Amaxa, Nucleofector, Nucleofection, Nucleocuvette, maxGFP and 96-well Shuttle are trademarks of Lonza Cologne GmbH in Germany and / or the U.S. and / or other countries. Other product and company names mentioned herein are the trademarks of their respective owners.