Related Manuals for Lonza PyroTec PRO
Summary of Contents for Lonza PyroTec PRO
- Page 1 Return to TOC Hardware User’s Manual PyroTec™ PRO Hardw are User’s Manual, V1.2 RT-MN024-1.2 02/21...
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Page 2: Preface
Before performing any work on or with PyroTec™ PRO, first read this User’s Manual care- Safety fully, in particular chapter 2 “Safety.” 0.1 Manufacturer Address of Lonza Walkersville Manufacturer 8830 Biggs Ford Road Walkersville, MD 29414 0.2 Use of the Product 0.2.1 Intended Use... -
Page 3: Fcc Rules
Return to TOC Go to TOC According to the ICES-001 notice the following statement applies to the PyroTec™ PRO: Radio Interference English Canadian Radio Interference Regulations ICES-001 Notice for Industrial, Scientific and Medical Radio Frequency Generators: This ISM apparatus meets all requirements of the Canadian interference-causing equip- ment regulations. -
Page 4: Table Of Contents
Table of Contents Preface ..................2 0.1 Manufacturer ....................2 0.2 Use of the Product ..................2 0.2.1 Intended Use..................2 0.2.2 Improper Use ..................2 0.3 CE Conformity ....................2 0.4 CSA Certification ..................2 0.5 FCC Rules ....................3 About this Manual ................ - Page 5 4.1.1 PyroTec™ PRO Overview..............24 4.1.2 Product Identification and Labelling ........... 25 4.2 Configuration Data ..................28 4.2.1 Air LiHa Configuration ............... 28 ® 4.2.2 Tecan Sunrise™ Reader and Loading ID ......... 29 4.3 Requirements ..................... 29 4.3.1 Computer Requirements ..............29 4.3.2 Software Requirements..............
- Page 6 Note: Unless otherwise specified a contracted field specialist will perform this procedure....................51 6.3.1 PyroTec™ PRO Instrument ............... 51 6.4 Checking the CA N Bus Resistance .............. 51 6.5 Installing Options ..................53 6.5.1 Modifications on Safety Panels ............53 6.5.2 Mains Outlets for Options ..............
- Page 7 9.1.1 Cleaning Agents ................81 9.1.2 For Air LiHa Maintenance..............83 9.2 Maintenance Schedule................83 9.2.1 Maintenance: Immediate Maintenance ..........84 9.2.2 Maintenance Table: Daily Maintenance..........85 9.2.3 Maintenance Table: Weekly Maintenance .......... 85 9.2.4 Maintenance Table: Yearly Maintenance..........86 9.2.5 Maintenance Table: Two-Yearly Maintenance ........
- Page 8 11.2.2 Packaging ..................109 11.3 Storage ....................109 Disposal................. 110 12.1 Disposal of Packing Mat erial..............110 12.2 Disposal of Operating Material ..............110 12.3 Disposal of the PyroTec™ PRO..............110 12.3.1 General Instructions................ 110 12.3.2 Local Requirements European Union..........111 12.3.3 Local Requirements People’s Republic of China.......
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Page 9: About This Manual
Return to TOC 1 About this Manual Purpose of This chapter describes the purpose of the manual, specifies the product the manual deals This Chapter with and whom the manual is intended. It explains the symbols, conventions and abbrevi- ations used and offers other general information. Purpose of This manual describes the PyroTec™... -
Page 10: Reference Documents
(for simplicity reasons, the symbols for trademarks, such as ® and ™ are not repeated later in the manual): • WinKQCL™ and PyroTec™ PRO are trademark of Lonza Walkersville in major countries ® ® Tecan® Freedom EVOware and Freedom EVO are registered trademarks and Sun- •... -
Page 11: Abbreviations
Return to TOC 1.3 Abbreviations Air LiHa Air displacement pipetting arm cLLD Capacitive liquid level detection Coefficient of variance or variation DiTi Disposable tip DMSO Dimethyl sulfoxide European Norm ETFE Ethylene/Tetrafluoroethylene-copolymer Tetrafluoroethylene/Perfluoropropylene-copolymer FFPM Perfluoroelastomer Field service engineer Liquid handling LiHa Liquid handling arm Multichannel arm MCA96... -
Page 12: Safety
Return to TOC Polystyrene vPTFE Polytetrafluoroethylene Polyvinylchloride PVDF Polyvinylidenefluoride RoMa Robotic manipulator arm Radio frequency Universal serial bus Uninterruptable power supply World Health Organization 2 Safety Purpose of This chapter describes the safety concept of PyroTec™ PRO, provides general rules this Chapter of correct behavior, and warnings concerning hazards associated with the use of the PyroTec™... -
Page 13: Product Safety
Return to TOC WARNING Radioactive radiation WARNING Fire hazard WARNING Electrical danger WARNING Crushing hazard WARNING Laser hazard WARNING Explosive material ATTENTION ATTENTION notes appear as follows Symbols ATTENTION With the general “Read This!” symbol, ATTENTION indicates the possibility of equipment damage, malfunctions, or incorrect process results, if instructions are not followed. -
Page 14: Instrument-Related Hazards And Safety Measures
Authorized modifications to the system may only be performed by an FSE cer- tified for the repair and upgrading of the PyroTec™ PRO. o Lonza will reject any claim resulting from unauthorized modifications. Fire hazard can be caused by the improper use of the PyroTec™ PRO. - Page 15 Return to TOC o Equipment should be connected to a grounded power source using an ap- proved power cord with grounding conductor. o Do not remove covers and other parts protecting from electricity. o Always keep the areas of electric parts, such as power supply plug, mains switch, etc., dry.
- Page 16 Return to TOC o Always be aware of possible hazards associated with these substances. o Use appropriate protective clothing, safety goggles and gloves. o The handling of substances and the disposal of waste may be subject to local, state, or federal law, or to regulations with regard to health, environment, or safety.
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Page 17: Operating Company
• Have read and understood the instructions in the User’s Manual. • Lonza recommends that the operator attend an operator-training course including routine use and end-user maintenance training. Please ensure training requirements are stated clearly on a completed pre-installation checklist. -
Page 18: Safety Elements
3.2.3 Modifications on the Safety Panels Some options for the PyroTec™ PRO require modifications on the safety panels. These modifications must be performed by a Lonza approved FAS (field application specialist) when the option is installed. WARNING If the options, which require modifications on the PyroTec™... - Page 19 Return to TOC PyroTec™ PRO with Standard Front Safety Panel Safety elements / standard (open) front safety panel Standard front safety panel Top safety panel Door lock Loading interface (optional) Side safety panel Cutout for continuous loading PyroTec™ PRO with Closed Front Safety Panel (Option) Safety elements / closed front safety panel (option)
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Page 20: General
Return to TOC PyroTec™ PRO with Front Safety Panel with Adjusta- ble Access Window (Op- tion) Safety elements / front safety panel with adjustable access window (option) Front safety panel Side safety panel Adjustable access window Top safety panel Door lock 3.3 General Removal of The protective and safety devices installed on the PyroTec™... -
Page 21: Product Safety Signs
Return to TOC 3.3.1 Product Safety Signs Where are PyroTec™ PRO Instrument Safety Notices Attached? The figure shows the safety notices attached to the PyroTec™ PRO instrument. It also shows their locations: Fig. 2-6 Safety notices attached to the product The following table explains the significance of the notices Tab. -
Page 22: Laser Radiation
• • Before a Lonza approved FAS (field application specialist) performs any on-site work on the instrument. Before the instrument or parts of it are returned to Lonza (e.g. for repair). • • Prior to storage of the instrument. •... -
Page 23: General Safety Rules
Contact the Lonza Scientific Support for further information. Note: Lonza reserves the right to refuse any instrument or a part of it, or will charge an extra fee, if the decontamination is not declared sufficiently 3.5 General Safety Rules... -
Page 24: Technical Data
PyroTec™ flexible platform. PRO? Delivery The PyroTec™ PRO is delivered only to Lonza authorized field service engineers, who take responsibility for assessing and investigating each installation at an end-user site to com- ply with local requirements. 4.1.1 PyroTec™ PRO Overview Illustration 1: PyroTec™... -
Page 25: Product Identification And Labelling
Return to TOC 4.1.2 Product Identification and Labelling Type plate Details for product identification can be read from the type plate, which is located on the back of the instrument near the power inlet. On the type plate (A) you find the following information: Identification data •... - Page 26 Return to TOC Serial Number The identification data is also printed on the serial number label (B). This label can be Label viewed from the front side of the instrument and is attached below the diluters. Instrument Instrument equipped with: Instrument height: Heights Air displacement pipetting arm, Air LiHa...
- Page 27 Uninterrupti- For an optimal operation of instruments and smooth running of the relevant application, ble Power Lonza recommends connecting an online UPS, so that the power supply runs via the UPS Supply (UPS) with a filter effect. UPS recommendations:...
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Page 28: Configuration Data
Field upgrades can be performed by a Lonza approved FAS (field application specialist) only. Delivery The PyroTec™ PRO is delivered only to a Lonza approved FAS (field application specialist), who take responsibility for assessing and investigating each installation at an end-user site to comply with local requirements. -
Page 29: Tecan Sunrise™ Reader And Loading Id
Return to TOC ® 4.2.2 Tecan Sunrise™ Reader and Loading ID The pictures below show the possible Tecan® Sunrise™ readers and Loading ID configura- tions: This manual is applicable for: PyroTec™ PRO - Part No. 25-A10; 1 Plate instrument without loading ID PyroTec™... -
Page 30: System Modules
Return to TOC 4.4 System Modules The system modules are briefly introduced in the following sections. According to your or- der configuration, some of these options might be installed. 4.4.1 Air Displacement Pipetting Arm (Air LiHa) The PyroTec™ PRO is equipped with an Air LiHa which is a robotic arm having multiple pi- pette tip for general pipetting tasks. - Page 31 • from 9 mm ± 0.4 mm Movement • to 38 mm ± 1 mm Disposable Use only Lonza disposable tips. Conductive disposable tips are available with filter, in fol- Tips lowing volumes: • 1000 µl DiTi Carrier A DiTi Carrier holds up to three trays of 96 disposable tips.
- Page 32 Pipetting Precision Prerequisites Based on Lonza quality control requirements, the values in the tables below are only valid if maintenance instructions and schedule have been followed strictly. Definition Precision is calculated as coefficient of variance (CV %). Typical pipetting precision is de- fined as the median value of the CV (calculated over all channels) of all tested instruments.
- Page 33 Return to TOC Pipetting conditions Temp. 20°C to 27°C/68°F to 80.6°F, relative humidity 30% to 60% • (non-condensing). • Tap water with a conductivity of 0.3 mS/cm to 1 mS/cm Free dispense; single pipetting mode, complete range from 0.5 µl to 1000 µl •...
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Page 34: Robotic Manipulator Arm (Roma)
Return to TOC Factory and Field Verification Values Test General test conditions, carried out with the Setup & Service Software: Conditions • All liquid handling values have been verified under controlled laboratory environ- ment at temperatures between 20°C and 27°C and a humidity between 30 % and 60 % at 25°C. -
Page 35: Chemical Resistance
Return to TOC ATTENTION Improper transport of labware (microplates, etc.). Use only labware that is rigid enough not to be deformed by the gripper force. 4.5 Chemical Resistance Chemical In the following table the chemical resistance of the used (standard) materials is specified: Resistance Chemical resistance tab le Material... -
Page 36: Resistance Of Special Materials
Return to TOC Sodium hydroxide Perchloric acid 60 % Petroleum ether 30/50 Hydrochloric acid 32 % Trichloroacetic acid 40 % Legend: Resistant partly resistant, use is possible with frequent replacements not resistant, unsuitable for use not determined 4.5.1 Resistance of Special Materials 4.5.1.1 Air LiHa Tip Cone The Air LiHa tip cone and the inline filter may be moistened with aerosols from the sample... -
Page 37: Description Of Function
Return to TOC 5 Description of Function Purpose of This chapter explains the basic principle of the PyroTec™ PRO, shows how it is structured This Chapter and gives a functional description of the assemblies. 5.1 Introduction Components The instrument consists of a platform that includes worktable, frame, housing, main elec- tronic boards, and power supply. -
Page 38: Function
Return to TOC 5.3 Function 5.3.1 Air Displacement Pipetting Arm (Air LiHa) Purpose of Air The Air LiHa is a pipetting arm for general pipetting tasks. Aspiration and dispense of liq- LiHa uids are based on air displacement. Servo motors move the tips in X, Y or Z-direction. Movements The pipetting tips can be moved independently in Z-direction. - Page 39 Return to TOC The Pipetting Each pipetting channel of the Air LiHa is equipped with an individual plunger drive to vary System the volume of the air chamber in the Z-rod. The figure shows two Z-rods in spread position to show how they are arranged. The plunger drives are alternately mounted on the right or left side for odd an even-numbered channels.
- Page 40 Return to TOC Function The figure shows the main parts and the work principle of the Air LiHa systems air chamber and plunger drive. Principle of the Air LiHa plunger drive Encoder Plunger Motor Pressure sensor Gears Tip adapter Air chamber Threaded spindle Inline filter Seal...
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Page 41: Air Liha Multisense Functions
Return to TOC During dispense, the spindle rotates in reverse direction, the plunger moves down and presses the liquid out of the tip. Air Chamber In the lowest position, the plunger end is close to the disposable tip to keep the dead vol- ume in the air chamber low. -
Page 42: Robotic Manipulator Arm Standard (Roma Standard)
Return to TOC Pressure- The pLLD function measures the pressure changes in the tip as the tip moves down. As Based Liquid soon as the tip touches the liquid surface, the pressure change triggers a detection signal. Level pLLD records the height of the tip at the time of the trigger signal. Detection pLLD can be used as an alternative to the capacitive liquid level detection (cLLD), e.g. -
Page 43: Safety Elements
Return to TOC 5.3.4 Safety Elements Front Safety The front safety panel is secured in closed position with the door locks. Two gas springs fa- Panel cilitate the opening of the panel. Functions of The standard front safety panel has the following function: Safety Panel Restrict access to moving parts (moving parts, mechanical hazards) •... -
Page 44: Reader
Return to TOC 5.4 Reader The following microplate reader type is supported and always mounted on the instrument worktable: Tecan® Sunrise™ • The microplate is loaded and unloaded by means of a RoMa arm. For more information about the microplate reader, refer to the reader’s documentation. 5.4.1 Capacitive Liquid Level Detection How Does it The integrated capacitive liquid level detection (cLLD) measures the capacitance between... -
Page 45: Clot Detection
Return to TOC Appropriate message if no liquid or not enough liquid available for sampling • • Software controlled, constant submerge depth during aspiration and dispensing • Enabling clot detection 5.4.2 Clot Detection How Does it The clot detection is based on the liquid level detection. The application software moni- tors the exit signal while the tip is retracted after aspiration of a liquid and compares the Work? level at which the exit signal appears with the liquid level detection value. - Page 46 Return to TOC If There Is a The clot detection generates an error message during retraction of the tip in two situa- tions. In both situations, a clot sticking to the tip or clogging the tip is the most possible Clot Detected cause for the non-appearance of the exit signal within the limit.
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Page 47: Lower Diti Eject Option
Return to TOC Limits of the The following critical situation may occur if the sample has not been centrifuged properly. Clot Detection 1) There are floating particles in the sam- ple. The tip aspirates liquid. 2) During aspiration (in the worst case shortly before the end of the aspira- tion process), a particle clogs the tip (see arrow). -
Page 48: Installation
Return to TOC Carrier PyroTec™ PRO utilizes a fixed deck layout. The carriers should be used in the same position Positioning where they are left during installation. Barcode Barcodes on the carriers and on most of the individual containers can be identified. Identification 6 Installation This chapter describes:... - Page 49 Return to TOC Note: though the instrument works perfectly at this minimum distance to the wall, be aware of the fact that it is an advantage if the rear of the instrument is accessible, e.g. for maintenance and service work. Weights Load capaci- ties...
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Page 50: Unpacking
Return to TOC 6.2 Unpacking Check before Note: Unless otherwise specified a contracted field specialist will perform this procedure. unpacking Check the following, before unpacking the instrument: Is the packaging damaged in any way? • • Is the instrument damaged in any way? Is the delivered material complete according to the “Order Configuration/Packing List”... -
Page 51: Installing And Connecting
Return to TOC 6.3 Installing and Connecting Note: Unless otherwise specified a contracted field specialist will perform this proce- dure. 6.3.1 PyroTec™ PRO Instrument Checks Movement To check the mechanics, manually move the axes of all modules and options slowly to Check their end positions. - Page 52 Return to TOC CAN Bus The CAN bus termination must be adapted to the individual configuration of the instru- Termination ment. For this reason, most PCBs have either jumpers or switches to connect the termina- tion resistors, where appropriate. In every case two participants of a CAN bus string must be terminated. The example below shows how the implementation of an additional module (e.g.
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Page 53: Installing Options
6.5.1 Modifications on Safety Panels Modifications Some options for the PyroTec™ PRO require modifications on the safety panels. These on the Safety modifications must be performed by an authorized Lonza FSE (field service engineer) when Panels the option is installed. WARNING If the options require modifications on the PyroTec™... -
Page 54: Mains Outlets For Options
The installation procedure depends on whether the Sunrise™ reader is delivered together Details with a new Lonza PyroTec™ PRO instrument or whether it is added later. If it is added later, the instrument must be upgraded before the Sunrise™ reader can be installed. -
Page 55: Site Acceptance
Return to TOC 6.6 Site Acceptance Completion A Lonza approved FAS (field application specialist) should complete all IQOQPQ forms and documentation • The Customer should sign all IQOQPQ documents as acceptance of the installation completion 7 Transport, Storage and Packaging Purpose of This chapter instructs how to shut down the PyroTec™... -
Page 56: Storage
Return to TOC How to Lift Lifting the Instrument To lift the instrument, the transport handles must be applied to the base structure of the Instrument instrument. Consider the safety instructions above. A – Transport handle B – Bar C – Pin To apply the transport device, proceed as follows: Attach all four transport handles •... - Page 57 Return to TOC The packaging consists of A wooden pallet with specially shaped foam parts • • The cardboard box for accessories A packaging strap, edge protection, buckle and brackets to fix the cardboard box to • the X-bay • The outside wooden box with a lid, packaging straps •...
- Page 58 Return to TOC To pack the PyroTec™ PRO, proceed as follows: How to Pack the PyroTec™ • Put the PyroTec™ PRO on the pallet as shown in the figure: Transport • Mount the transport locks (B) on either Locks side of the PyroTec™ PRO. o Lower part: Fix the transport lock with the screw (C).
- Page 59 Return to TOC Protect the arms with the correspond- • ing foam block. • Move the RoMa, the PnP gripper head and the CGM rotator downwards in such a way that they fit into the recess of the appropriate foam block. With the PnP arm and the CGM the Z- brake must be loosened to move the PnP head and the CGM rotator into...
- Page 60 Return to TOC Place the two cardboard strips (A) onto • the worktable to prevent it from being scratched by the accessory box. Fixing the Prepare the packaging strap as follows: • Accessory o Cut two pieces of packaging strap to a length of approx. 1m (40 in.).
- Page 61 Return to TOC Place the accessory box (A) into the gap • as shown in the figure. • Fasten the accessory box as follows: o Attach the straps to the buckle (A) as shown in the figure. o At the corner of the accessory box, apply edge protection (A) between the strap and the box as shown in the figure.
- Page 62 Return to TOC Place the outer wooden box (B) on the pallet • Put corner protection blocks (A) into place. • • Place the lid (A) on top of the packaging. • Apply 3 packing straps. • Stick the dispatch label to the front side of the packaging (see arrow). PyroTec™...
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Page 63: Operation
Unpacking To unpack the PyroTec™ PRO, follow the instructions for packing in reverse order. Note: Lonza recommends keeping the original packaging for future transport or storage of the PyroTec™ PRO. The packaging has been carefully designed to prevent damage to the instrument or its parts. -
Page 64: Display Elements
Return to TOC the power ON/OFF switch are not pressed unintentionally. Before pressing the pause button or the power ON/OFF switch, make sure that you • will press the intended button. • Before pressing the pause button in order to pause a process, make sure that the in- strument is running. -
Page 65: Operating Modes
For service mode, refer to the “Instrument Software Manual”. • Setup and service mode (a Lonza approved FAS (field application specialist) ) o Serves to set up the instrument, to make adjustments and to run tests. PyroTec™ PRO Hardw are User’s Manual, V1.2... -
Page 66: Operating In Routine Operation Mode
Return to TOC 8.3 Operating in Routine Operation Mode 8.3.1 Safety Instructions WARNING Automatically moving parts. Injuries (crushing, piercing) possible if the safety panels are not in place or if the standard front safety panel is installed. The standard front safety panel is partially open, allowing access to the worktable and continuous load Before starting the PyroTec™... - Page 67 Return to TOC ATTENTION Instruments are intended for indoor operation with controlled temperatures. DiTis may leak due to pipetting liquids with high vapor pressure. It is important to maintain constant temperature plus air gaps. ATTENTION Possible malfunction due to tip clogging. Using liquids with undissolved particles could lead to clogged tips and thus result in liquid not being dispensed.
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Page 68: Enclosed Work Area
Return to TOC In the event of power failure or an otherwise aborted run, all partially processed samples should be discarded. Do not attempt to restart an interrupted program unless the com- puter screen displays explicit instructions for resuming operation. Chemical, Biological and Radioactive Hazards WARNING All samples and test kit components must be considered potentially hazardous agents. - Page 69 Return to TOC WARNING Automatically moving parts. If the safety panels are not in place, injuries (crushing, piercing) are possible. Before starting the PyroTec™ PRO, make sure that the safety panel is closed. Never operate the instrument with the panel open. To switch the PyroTec™...
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Page 70: Instrument Preparation And Checks
Check the disposable tip rack and add tips if necessary. • • Make sure that all carriers and labware are properly positioned in accordance with the Lonza PyroTec™ PRO deck layout. Ensure that the daily maintenance has been carried out according to the maintenance • chapter. - Page 71 Return to TOC ATTENTION Improper initialization of robotic arms. The robotic arms cannot initialize property if there is an object, such as a lost sample tube or a tool, etc., between the arm and the initial stop position. • Make sure that there are no unwanted objects present in the instrument. Check the arm position after the initialization command.
- Page 72 Return to TOC Make sure that the correct rack is used for the carrier. If a carrier is damaged, replace it immediately. Carrier ID Each carrier ID must be unique. WARNING Wrong identification of a carrier If carriers are not placed correctly on the worktable and if there are unfavorable circum- stances (barcode labels not within specified limit, distance incorrectly placed carrier to the barcode reader still enables reading), the barcode reader might read the wrong carrier.
- Page 73 Make sure that the tip lengths of the DiTis present on the worktable correspond with the ones defined in the application software. ATTENTION DiTi crash/erroneous pipetting results due to incorrect DiTi types. The PyroTec™ PRO is designed to use 1mL conductive filter DiTis (Lonza P/N • 00229884). •...
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Page 74: Runtime Controller
The runtime controller of the application software controls The PyroTec™ PRO. With the runtime controller, the following tasks are performed during installation and maintenance where appropriate. Runtime controller should only be accessed by Lonza ap- proved engineers: PyroTec™ PRO Hardw are User’s Manual, V1.2... -
Page 75: Checks And Terminating Tasks
Return to TOC Login for lab operator, application specialist or administrator: • o The software only allows authorized users with a valid login to perform any ac- tion on the instrument. Starting a process run. • • Performing maintenance. • User management: o Allows the administrator to set up users with the corresponding access rights. -
Page 76: Operating In Process Definition Mode
The device must be validated by qualified technical personnel (e.g. Lonza approved engi- neer or technician) before putting into service and after changes. Use of kits or kit compo- nents on PyroTec™ PRO is only allowed after validation by Lonza, the kit manufacturer or operator of the system. - Page 77 Return to TOC Use of Test Tubes In the application software, the following rack parameters for test tubes are predefined: Striprack Test tube diameter, outside Standard rack 13 mm Note: In the case of deviating parameters, these values must be adjusted in the applica- tion software.
- Page 78 Return to TOC Sample tub e Z-heights Z-travel Z-max Z-start, Z-dispense Reagent trough Z-heights Z-travel Z-max Z-start, Z-dispense Microplate Z-heights Z-travel Z-max Z-start, Z-dispense Note: The use of deviating parameter values must be discussed with the responsible appli- cation specialist. PyroTec™...
- Page 79 DMSO, ethanol, distilled water very high ATTENTION Full system functionality can only be guaranteed when Lonza certified disposable tips are used ATTENTION Contamination of the 3/2-way valve. Make sure not to aspirate sample, or other liquid that may contaminate the valve, into the 3/2-way valve.
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Page 80: Maintenance
Return to TOC 8.4.2.3 LiHa Arm High Density Vibrations, caused by the movement of other arms may result in positioning difficulties in Applications high-density applications. If you encounter such difficulties, please contact your local service organization. In such applications, where the mechanical precision is very critical, speed and accelera- tion of the arm movements may be adapted accordingly. -
Page 81: Preventive Maintenance And Repairs
Return to TOC 9 Preventive Maintenance and Repairs Purpose of This chapter gives instructions on all maintenance work to be performed in order to keep This Chapter the PyroTec™ PRO in good working condition. In addition to this, adjustment, and repair jobs the operator can carry out by himself/herself are explained. - Page 82 Return to TOC Bacillol Plus Alcoholic, disinfection agent, Bode Chemie, Hamburg Please contact the free of formaldehyde, for sur- www.bode-chemie.de manufacturer face cleaning DNAzap Cleaning agent for surfaces Ambion Please contact the contaminated with nucleic ac- www.ambion.com manufacturer Kohrsolin Surface disinfectant Bode Please contact the www.bode-chemie.de...
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Page 83: For Air Liha Maintenance
Return to TOC Use: Decon / Contrad for surface cleaning only Do not use: Decon / Contrad, Bleach, Peraclean, SporGon as clean- ing bath for carriers (damage to aluminum) Racks Water, alcohol, weak detergent, disinfectant Gripper Water, alcohol, weak detergent, disinfectant Safety panels Water, alcohol, disinfectant, suitable for acrylic glass Disposable tip cones... -
Page 84: Maintenance: Immediate Maintenance
Return to TOC Example and Example for a maintenance table, followed by explanations: Explanation Example (e.g. daily maintenance) Instrument/ Component Maintenance Task Reference Part A Clean thoroughly Water with weak detergent Part B Check adjustment of compo- Refer to section X.X.X, Y-Z nent C Instrument/Component •... -
Page 85: Maintenance Table: Daily Maintenance
Return to TOC 9.2.2 Maintenance Table: Daily Maintenance At Beginning Instrument/Component Maintenance Task of Day DiTi cones (LiHa) Clean Check for deposits Tighten Adjustment check Air displacement Clean and inspect DiTi cones pipetting arm (Air LiHa) RoMa Visually check grippers for deformities and damage During Day Instrument/Component Maintenance Task... -
Page 86: Maintenance Table: Yearly Maintenance
The weekly maintenance should be performed on the last working day of each week. 9.2.4 Maintenance Table: Yearly Maintenance Annual maintenance will be performed by a Lonza approved FAS (field application specialist) and be offered through a service contract. Reference material for the maintenance tasks can be available upon request. -
Page 87: Maintenance Table: Two-Yearly Maintenance
Prior to loading disposable tip trays into the rack and onto worktable, make sure that the DiTis are faultless and clean. • Ensure that only regular and straight Lonza disposable tips are being used. • Inspect the DiTi box for traces of microbial contamination. - Page 88 Return to TOC 9.3.1.1 Disposable Tip Cone (DiTi cone) LiHa WARNING Possible contamination. The space between the disposable tip cones and the tubing extension can become mois- tened with sample liquid and thus create a contamination risk. Decontaminate the entire equipment thoroughly before maintenance work. •...
- Page 89 Return to TOC Replacing DiTi This section describes the replacement of the DiTi adapters. Adapter Preparation To prepare for replacement, proceed as follows: • Switch the instrument off. • Open the front safety panel. Manually move all Z-rods up to their topmost position. •...
- Page 90 Return to TOC Z-rod Tubing extension Tip adapter O-ring Thread Separator ring (white) Pipetting tubing DiTi cone Adapter cylinder Tip ejector tube (outer rim pointing upwards) • Carefully pull the pipetting tubing approximately 25 mm (1 in) out of the tip adapter. Put the adapter cylinder on the tubing extension (knurled part pointing upwards).
- Page 91 • To ensure operating readiness, perform the following test: Settings o Instrument Warm-up Refer to the PyroTec™ PRO Software User Guide. Please contact your local Lonza representative to schedule a service visit. Replacement 9.3.1.3 Air LiHa Inline Filter 9.3.1.4 Note: Irrespective of the regular maintenance schedule, the filter needs replaced if mois- tened because of an erroneous aspiration (too much liquid aspirated).
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Page 92: Diti Waste Bag
Return to TOC • Carefully pierce the old filter side- ways, using the filter removal tool (A). • Swivel the tool downwards to pry out the filter. Remove the filter from the DiTi • cone. Inline filter removal • Clean the DiTi Cone with alcohol and allow to dry. •... - Page 93 Return to TOC WARNING Risk of fire or explosion If inflammable reagents were used in the process, remains of these substances on the waste DiTis may accumulate and form combustible vapors. • If inflammable reagents are in use, change the DiTi waste bag frequently. Perform a risk assessment to define further measures.
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Page 94: Diti Waste Station Unit
Return to TOC Typical dimensions for the waste bag (W x L) 300 mm x 600 mm Waste Bag Thickness: 0.05 mm Specification Material: Polypropylene, polyethylene, or co-polymer (auto-clavable) Imprint: Biohazard Note: The waste bags used must meet your local safety guidelines. 9.3.3 DiTi Waste Station Unit WARNING Potentially infectious... - Page 95 Return to TOC Spray some disinfectant on the inner • surface of the DiTi waste slide as shown in the figure. • Suitable agents: Check if there are residues of contami- • nation on the inner surface of the DiTi waste slide.
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Page 96: Worktable
Return to TOC To remove and clean the DiTi waste station unit, proceed as follows: Removal • Remove the DiTi waste bag housing • Remove the DiTi waste slide inset. Removing the DiTi waste station unit from the worktab le Pull and hold the quick release lever (B). -
Page 97: Safety Panels
Return to TOC Wipe the surface of the worktable with a suitable cleaning (e.g. alcohol, disinfectant) • to remove any spilled reagent. • If necessary, clean with additional water. 9.3.5 Safety Panels Cleaning the Perform the following procedure to clean the safety panels. Safety Panels Wipe the inner and outer surface of the safety panels with a suitable cleaning agent, •... -
Page 98: Precision And Function Tests
Lonza recommends the use of the QC kit for liquid handling performance verification test- ing at least once per year during Preventative Maintenance by a Lonza approved Field Ap- plication Specialist; a higher frequency may apply, based on the Quality & Regulatory re- quirements of the laboratory using the PyroTec™... -
Page 99: Decontamination
Return to TOC 9.5 Decontamination Agents Note: The selection of the appropriate decontamination agent depends on the contamina- tion degree and the kind of contaminant. Decontamination can be performed with the following agents: Bleach 0.5% to 3% • • 70% ethanol + 30% H Commercially For commercially available agents that can be used for decontamination or disinfection. - Page 100 Return to TOC Carefully press the new pin into the • hole on the worktable. You can use a small rubber mallet if the pin cannot be inserted manually. Inserting a positioning pin Note: When replacing positioning pins, take note to the following: Make sure to only replace a pin by one of exactly the same type and pay attention to •...
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Page 101: Troubleshooting
Correct this, appropriate corrective measures are listed in the column “Corrective measures”. The Lonza FSE according to separate instructions usually performs the elimination of more complicated malfunctions or errors. In this case, reference to the FSE is made. 10.1 Troubleshooting Table... - Page 102 Dirty DiTi cone Clean DiTi cone See “Instrument Parts and Cleaning Agents” MultiSense option: Pres- Contact Lonza Scientific Support sure measurement bad Not enough liquid detected Not enough liquid Check/add liquid Incorrect container/rack Check container, rack definition definition...
- Page 103 (1 in.) See “Releasing the Z-brake of the Air LiHa” Air LiHa tip adapter crash Tip adapters not aligned Contact Lonza Scientific Support for correctly re-aligning the tip adapters Problem, error on multichannel arm Tips/DiTis are not aligned The carrier offsets are not...
- Page 104 Contact Lonza Scientific Support. DiTi position is defective. Several/all DiTis are not Wrong DiTis are used Use DiTis provided by Lonza only dropped If the problem cannot be solved, contact Lonza Scientific Support. The DiTi box is lifted up with...
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Page 105: Troubleshooting Instructions
Return to TOC 10.2 Troubleshooting Instructions 10.2.1 Releasing the Z-brake of the Air LiHa The Air LiHa is equipped with a Z-break (electrical actuation of release) to prevent the Z- rods from moving down by their weight when the power is off. Manual Acti- The Z-brake of the Air LiHa can be released manually, when the instrument is switched off. -
Page 106: Roma / Gripper Alignment
Return to TOC In principle, the Z-rods tend to move downwards. Make sure that the tip adapters and tips do not collide with any objects below. Release the armature when the tip adapters and tips are in the desired position. •... -
Page 107: Shutdown, Transport And Storage
11.1 Shutdown 11.1.1 Instrument Since the material processed by the PyroTec™ PRO is unknown to Lonza, detailed infor- mation on how to dispose of it cannot be given here. WARNING Chemical, biological, and radioactive hazards can be associated with the waste material from the process run on the PyroTec™... -
Page 108: Reporting
Return to TOC • Unplug the power cord from the power supply at the rear of the instrument. RS232 interface on Optib o PCB RS232 interface connector USB connector • Disconnect the instrument from the PC. If desired, unplug the interface cable from the USB port on the Te-CU board behind •... -
Page 109: Unpacking
Lifting or moving the instrument must be correctly prepared and may only occur under the direction of a qualified Lonza person only The transport of the instrument may be done under direction of a qualified Lonza person Transport only. Due to the heavy weight, trained relocation staff is needed to lift the instrument. -
Page 110: Disposal
12.3 Disposal of the PyroTec™ PRO 12.3.1 General Instructions WARNING Depending on the application, parts of the PyroTec PRO may have been in contact with biohazardous, poisonous or even radioactive material. Make sure to treat this material according to the applicable safety standards and regu- •... -
Page 111: Local Requirements European Union
In accordance with the requirements specified in SJ/T11364-2014, all electronic and elec- Marking trical Lonza products sold in the People’s Republic of China are labeled with a marking for the restriction of the use of hazardous substances. Marking for the restriction of the use of hazardous sub stances... -
Page 112: Spare Parts And Accessories
Look up the ordering information on the tables. Spare Parts How to Order Order the parts from Lonza. Always state the designation and the part number when or- Spare Parts dering spare parts. Note: This chapter only contains spare parts that can be replaced by the operator him/her- self. - Page 113 Return to TOC 30017613 TOOL REF- ERENCE INSERT CALIBRATION ASSY ROMA-3 ROMA-3 30013574 TIP REFER- ENCE PMP TIP REFERENCE PMP 30013576 TOOL MOUNTING X-RING TOOL MOUNTING X-RING 0.74/1.02 0.4/1.2 30041341 TOOL MOUNTING TOOL MOUNTING O-RING PMP O-RING PMP 30013578 BLOCK TEST LEAKAGE BLOCK TEST LEAKAGE PMP 10619001...
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Page 114: Tools, Gauges
Return to TOC 30157222 DONGLE EVOWARE USB PYRO- TEC™ PRO 10619517 WRENCH KIT ACCESSORY LONZA PYROTEC™ PRO: includes - 30157222 DONGLE CONE DITI EVOWARE USB PYROTEC™ PRO, OPTION 5 10700830 WRENCH FORK, 30006384 CAP PCE. SCREW, 10900333 SCREW FIL.HEX.M4*8 A2 BN1593 (no pic.) -
Page 115: Optional System Modules And Accessories
CARRIER ADDITIVE TROUGH 3 1/25 mm/ (0.98 in.) In use with 100 ml (10613049) or 3 trough PCE. MAX.100ML holders (10619626) Trough, Disposable, 100uL Lonza Reagent troughs 00229888 13.6.2 Carrier for Disposable Tips Plain Text Designation Label Designation Width DiTi waste slide and bag holder... - Page 116 Return to TOC Container for DiTi waste bag Trough holder Wash station Slot of lower DiTi waste DiTi waste slide DiTi waste station unit Carrier for DiTi racks (3 DiTi racks 1000 µl) PyroTec™ PRO Hardw are User’s Manual, V1.2 RT-MN024-1.2 02/21...
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Page 117: Carriers For Tubes
10613004 CARRIER 6*16 POS. TUBE 13MM 6/150/ (5.91 in.) Carrier for tub es (example for 16 tub es) 13.7 Tips Plain Text Designation Lonza P/N Liquid Handling Filtered Tips, 1000uL 00229884 PyroTec™ PRO Hardw are User’s Manual, V1.2 RT-MN024-1.2 02/21... -
Page 118: Support
14.1 Contacts Product Sup- If your Lonza product fails to function properly, if you have questions about how to use or port &Tech- maintain our products, or if you need to send an instrument to Lonza for repair or other nical Service service, please contact our Scientific Support. - Page 119 Return to TOC PyroTec™ PRO Hardw are User’s Manual, V1.2 RT-MN024-1.2 02/21...