3D Histech Pannoramic MIDI II 2.0.5 User Manual page 49

Pannoramic MIDI II 2.0.5 User's Guide
Multilayer mode
Select Single layer option if you want to scan a single layer of the slide.
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By choosing the Extended focus option, the software selects the sharpest image from each
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focus level for each image field, and combines them into one single image. Therefore those
parts that were blurry in auto/manual focus will also be sharp. This method guarantees the
maximum depth of sharpness, however, it reduces digitization speed.
Z-stack: Both for Extended focus and Z-Stack scanning methods, you can determine the
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desired number of focus levels with the Levels selection box. The current focus value is the
starting point. If you set 5 for example, one current, two focus levels above and below FOVs
are captured. Therefore, it is advised to choose an odd number for the focus levels. Separate
layers are created and stored this way, and can be opened with Pannoramic Viewer. The Z-
stack slide contains the extended focus layer.
Step size determines the spacing between focus levels. One step is around 0.2 µm.
Default settings are: Levels: 7; Step size: 5.
December 7, 2018 – Rev. 1
Figure 23 – Focus level and step size
3DHISTECH Ltd.
5.1.1 Profile Settings Panel
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