3D Histech Pannoramic MIDI II 2.0.5 User Manual page 48

Pannoramic MIDI II 2.0.5 User's Guide
It requires lots of time (since it cannot find focus neither on the edges,
nor on specks) to digitize coverslip edges. Select Remove coverslip
function to eliminate those areas.
Do NOT use Remove coverslip function on TMA slides! The layout of
TMA slides may mislead the detecting algorithm.
Fill holes inside the scanmap – If selected, the holes inside the specimen will also be
◦
scanned. Often these holes contain small sample objects that otherwise would not be
scanned.
Remove specks – If selected, the scanner does not digitize objects smaller than the
◦
predetermined size. This helps filtering specks and other uninteresting objects out. Set
the maximum size of specks not to be digitized by adjusting the slider.
Dilate mask – Adjust the slider to enlarge or decrease the scanned area. The edge is
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extended discretely by 100 µm each time.
Scanmap
A previously saved scanmap can be loaded and applied.
Use Threshold: Preview settings defined in the Microscope Controller Panel / Preview
•
window are applied as the basis of scanmap.
Load scanmap: This function is useful if the slides contain areas of interest that cannot be
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detected by the preview camera. This function along with saving profiles helps to scan all
slides with the most adequate settings.
To load a mask, click Load. It will display a drop-down menu. Select whether to load preview
mask or TMA grid.
Custom scanmap: The scanmap draws manually on the preview image is used for scanning.
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Use selection box restriction: If selected, only the area inside the box is scanned. It is useful if
you only want to scan the area that is equivalent with the size of the coverslip, so external
specks will not be scanned.
December 7, 2018 – Rev. 1
Caution!
Warning!
3DHISTECH Ltd.
5.1.1 Profile Settings Panel
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