Prerun Safety Checks; Rotor Preparation; Operation - Beckman Coulter J-Lite JLA 16.250 Instruction Manual

PRERUN SAFETY CHECKS

ROTOR PREPARATION

OPERATION

Read the
SAFETY NOTICE
the rotor.
1. Make sure that the rotor, lid, and all tubes or bottles and accesso-
ries are clean and show no signs of corrosion or cracking.
2. Inspect the rotor drive pins to ensure that they are not damaged
(see instructions on page 20). If the drive pins appear to be
damaged, contact Beckman Coulter Field Service.
3. Verify that the bottles or tubes being used are listed in Table 3.
4. Check the chemical compatibilities of all materials used. (Refer
to Appendix A in Rotors and Tubes, or Chemical Resistances,
publication IN-175).
For runs at other than room temperature, refrigerate or warm the
rotor before the run for fast equilibration.
1. Be sure that the metal threads in the rotor are clean and lightly
lubricated with Spinkote lubricant (306812). Also ensure that lid
O-rings are lightly but evenly coated with silicone vacuum grease
(335148).
2. Load filled containers symmetrically into the rotor. (Refer
to
BOTTLES AND TUBES
containers.) If fewer than six bottles or tubes are being run,
they must be arranged symmetrically in the rotor (see Figure 2).
Opposing containers must be filled to the same level with liquid
of the same density.
• Precool the rotor in the centrifuge or in a refrigerator before use—
especially before short runs—to ensure that the rotor reaches the
set temperature. A suggested precooling cycle is a minimum of
30 minutes at 2000 rpm at the required temperature.
at the front of this manual before using
on page 15 for information about
J-Lite JLA-16.250 Rotor
7