Beckman Coulter J-Lite JLA 16.250 Instruction Manual page 5

Fixed angle rotor used in beckman coulter j2 and avanti j series centrifuges
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DESCRIPTION
Lid
Small O-Ring
(893503)
Fluid
Containment
Annulus
Large
O-Ring
(893502)
Rotor
Body
This rotor has been manufactured in a registered ISO 9001 or 13485
facility for use in Beckman Coulter Avanti J and J2 series centrifuges.
The J-Lite JLA-16.250 fixed angle rotor (see Figure 1), rated for
16 000 rpm in Beckman Coulter Avanti J series centrifuges and
14 000 rpm in Beckman Coulter J2 series centrifuges, is designed
to hold up to six 250-mL bottles at a 25-degree angle to the axis of
rotation. The JLA-16.250 rotor develops centrifugal forces that can
efficiently pellet cells from large volumes, or cell particles from tissue
homogenates. Short column methods (such as partially filled tubes)
may also be used to purify large quantities of virus in a cushion
gradient.
The rotor and lid are made of aluminum and are anodized for corro-
sion resistance—the rotor is black and the lid is blue. The tie-down
knob in the rotor lid secures the rotor to the centrifuge drive hub.
O-rings made of Buna N rubber, located in the rotor lid, help to main-
tain atmospheric pressure inside the rotor during centrifugation if the
O-rings are properly lubricated. Pins in the rotor drive hole mate with
grooves in the centrifuge drive hub (older model centrifuges), or with
drive hub teeth (newer model centrifuges), to prevent the rotor from
slipping during acceleration and deceleration.
Figure 1. The JLA-16.250 Fixed Angle Rotor (dual-locking lid shown)
J-Lite JLA-16.250 Rotor
5

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