Beckman Coulter Avanti J-25 Instruction Manual
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Beckman Coulter Avanti J-25 Instruction Manual

High-performance centrifuge
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J325I-IM-7
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Avanti
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High-Performance Centrifuge
Instruction Manual

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Summary of Contents for Beckman Coulter Avanti J-25

  • Page 1 J325I-IM-7 ® Avanti J-25 ® High-Performance Centrifuge Instruction Manual...
  • Page 2 Symbol Simbolo Symbol Title Titel Titre Titulo Titolo Symbole Símbolo Dangerous voltage Gefährliche elektrische Spannung Courant haute tension Voltaje peligroso Pericolo: alta tensione Attention, consult accompanying documents Achtung! Begleitpapiere beachten! Attention, consulter les documents joints Atención, consulte los documentos adjuntos Attenzione: consultare le informazioni allegate On (power) Ein (Netzverbindung)
  • Page 3 Safety During Installation and/or Maintenance This centrifuge is designed to be installed by a Beckman Coulter field service representa- tive. Installation by anyone other than authorized Beckman Coulter personnel invalidates any warranty covering the instrument.
  • Page 4 Mechanical Safety For safe operation of the equipment, observe the following: • Use only the Beckman Coulter rotors and accessories designed for use in this centrifuge. • Do not exceed the maximum rated speed of the rotor in use. • NEVER attempt to slow or stop a rotor by hand.
  • Page 5 J325I-IM-7 January 2007 Avanti J-25 ® High-Performance Centrifuge Instruction Manual © 2007 Beckman Coulter, Inc. Printed in the U.S.A. JUC-0107-PDF...

  • Page 7: Table Of Contents

    Contents Page INTRODUCTION Certification ..........vii Scope of this Manual .
  • Page 8 Contents Page SECTION 3: OPERATION Summary of Run Procedures ....... . 3-2 Manual Run .
  • Page 9 Illustrations Page Figure 1-1. Single-Phase Electrical Requirements ......1-9 Figure 1-2. Three-Phase “Y” Electrical Requirements ..... . . 1-9 Figure 1-3.

  • Page 10: Introduction

    Introduction CERTIFICATION ® To ensure full system quality, Beckman Coulter Avanti J-25 I centrifuges are manufactured in a registered ISO 9001 facility. They have been designed and tested to be compliant (when used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable regulatory agencies.

  • Page 11: Conventions

    Further, the use of any equipment other than that recommended by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically recommended in this manual is the sole responsibility of the user.

  • Page 12: Radio Interference

    Introduction WARNING Used whenever an action or condition may potentially cause serious personal injury or loss of life. Mechanical damage may also result. WARNING Indicates high voltage or risk of electric shock. Refer servicing of all areas displaying this symbol to service personnel. TYPOGRAPHIC CONVENTIONS Certain typographic conventions are used throughout this manual to distinguish names of user interface components, such as keys and...

  • Page 13: Recycling Label

    For Beckman Coulter products bearing this label please contact your dealer or local Beckman Coulter office for details on the take back program that will facilitate the proper collection, treatment,...

  • Page 14: Specifications

    Specifications and Preinstallation Requirements SPECIFICATIONS Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data, without guarantee. CONTROL FEATURES Speed Setting range ....0 to 25 000 rpm (in 10-rpm increments) or equivalent RCF (in 100 ×...

  • Page 15: Operational Features

    Specifications and Preinstallation Requirements OPERATIONAL FEATURES Door......5.4-cm (2.1-in.) thick structural foam with steel plate Rotor chamber diameter .

  • Page 16: Available Rotors

    Specifications and Preinstallation Requirements AVAILABLE ROTORS Refer to the applicable rotor manual for complete rotor descriptions. Rotor Max RCF Manual Rotor Profile and Description (× g) Capacity Number JA-30.50 Ti Fixed Angle, 34° (8 place) 8 × 50 mL = 108 mm 25 000 75 600 J-TB-070...
  • Page 17 Specifications and Preinstallation Requirements Rotor Manual Rotor Profile and Description (× g) Capacity Number JA-20 Fixed Angle, 34° (8 place) 8 × 50 mL = 108 mm 20 000 48 400 J-TB-003 JA-18.1 Fixed Angle (24 place) 24 × 1.8 mL 45°...
  • Page 18 Specifications and Preinstallation Requirements Rotor Manual Rotor Profile and Description (× g) Capacity Number JA-12 Fixed Angle, 35° (12 place) 12 × 50 mL = 144 mm 12 000 23 200 J-TB-051 JA-10 Fixed Angle, 25° (6 place) 6 × 500 mL = 158 mm 10 000 17 700...
  • Page 19 Specifications and Preinstallation Requirements Rotor Manual Rotor Profile and Description (× g) Capacity Number JS-24.15 Swinging Bucket (6 place) 6 × 15 mL 10 000 19 200 J-TB-058 = 171.3 mm JS-13.1 Swinging Bucket (6 place) 6 × 50 mL 13 000 26 500 J-TB-036...

  • Page 20: Preinstallation Requirements

    The following information is provided in case the centri- fuge must be relocated. The centrifuge will be installed by a Beckman Coulter Field Service representative after preinstallation requirements for power and site preparation have been met. The following equipment is required for preinstallation: •...
  • Page 21 Specifications and Preinstallation Requirements Table 1-1. Nominal Supply Voltage Ratings Instrument Instrument Nominal Nominal Instrument Part Part Number Supply Power Cord and Plug Voltage Rating Number (Elutriation System) Frequency Description Single-phase, 363106 363114 185–264 V, permanently attached three- 200/208/240 V 50/60 Hz, 30 A wire UL/CSA-approved cord with NEMA type 6-30P plug...

  • Page 22: Figure 1-1. Single-Phase Electrical Requirements

    Specifications and Preinstallation Requirements 30-ampere Circuit Breaker Wall Outlet: Hubbell 9330, Bryant 96-30-FR, or Equivalent (NEMA 6-30 R) Measured Line Earth- Voltage Ground 30-ampere Circuit Breaker Figure 1-1. Single-Phase Electrical Requirements 16-ampere Circuit Breaker Measured Neutral Line Voltage Phase to Phase 16-ampere Circuit Breaker Earth- 16-ampere Circuit Breaker...

  • Page 23: Space And Location Requirements

    Specifications and Preinstallation Requirements Additional Requirements for Three-Phase Power Connections For three-phase power service to the centrifuge, use the “Y” connected three-phase configuration shown in Figure 1-3. Also note the following information: • The steady state current draw of the centrifuge can be as high as 12 amperes per phase, depending on the voltage.

  • Page 24: Figure 1-4. Rear View And Dimensions

    Specifications and Preinstallation Requirements • Allow a 7.7-cm (3-in.) clearance on each side of the instrument and a 16-cm (6.25-in.) clearance behind the instrument for air circulation. The centrifuge must have adequate air ventilation to ensure compliance to local requirements for vapors produced during centrifugation operation.

  • Page 25: Securing The Centrifuge To The Floor

    The holes must be drilled before your scheduled installation. USING J2 SERIES ROTORS IN THE AVANTI J CAUTION Do not use the Beckman Coulter JA-10, JS-7.5, JA-14, or JS-13 rotors in the Avanti J-25I centrifuge before reading this information. 1-12...

  • Page 26: Checking For Rotor Drive Pins

    (see Figure 1-5). These drive pins engage with the centrifuge spindle hub to ensure that the rotor does not slip during acceleration. Some Beckman Coulter rotors, including the JA-10, the JS-7.5, and the JA-14, were originally manufactured without drive pins because pins were not needed when these rotors were used in older model centrifuges.

  • Page 27: Using The Ja-18 Rotor

    If you do not see metal pins in the hole, do not use the rotor in the Avanti J. Call your local Beckman Coulter office for information on returning the rotor to the factory for upgrading.
  • Page 28 If the JCF-Z rotor is misidentified, the run speed will be limited to the maximum speed for the identified rotor. (The maximum speed for the JCF-Z rotor is 20 000 rpm.) In the United States, call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office. 1-15...

  • Page 29: Description

    CENTRIFUGE FUNCTION AND SAFETY FEATURES CENTRIFUGE FUNCTION The Avanti J-25 I is a refrigerated centrifuge that generates centrifu- gal forces required for a wide variety of applications. Together with the Beckman Coulter rotors designed for use in this centrifuge, applications include: •...

  • Page 30: Safety Features

    The centrifuge can also be operated by the SpinTrace™ II system for centrifuge control and data collection. SpinTrace II software runs on a personal computer connected to up to thirty-two centrifuges. Contact your Beckman Coulter representative for more information. SAFETY FEATURES Safety features include the following.

  • Page 31: Housing And Door

    Description • A steel casing surrounds the rotor chamber to provide operator protection in the unlikely event of a rotor mishap. • Dynamic Rotor Inertia Check (DRIC): As the rotor accelerates, rotor inertia is measured and the rotor energy is calculated for the speed set by the user.

  • Page 32: Drive

    Description DRIVE The drive spindle is directly driven by a brushless, high-torque, switched-reluctance drive motor. The instrument’s resilient suspen- sion minimizes disturbance of the sample during acceleration and deceleration, and reduces damage to the drive spindle if an imbalance occurs during centrifugation. FRICTION REDUCTION SYSTEM (FRS) The friction reduction system (FRS) uses a mechanical rotary vane vacuum pump to reduce chamber pressure to approximately one-...

  • Page 33: Overtemp System

    NAME RATING PLATE The name rating plate is affixed to the rear of the centrifuge. Always mention the model number and serial number when corresponding with Beckman Coulter regarding your centrifuge. CONTROLS AND INDICATORS POWER AND KEY SWITCHES The power switch and the key switch are located below the control panel (see Figure 2-1).

  • Page 34: Control Panel

    Description The power switch is a two-position rocker switch (I, on; O, off) which controls electrical power to the centrifuge. The key switch has five positions, indicating the five different operating modes. • NORMAL mode is used for regular, closed-door centrifugation. •...

  • Page 35: Figure 2-2. The Control Panel

    Description ROTOR ID SPEED TIME TEMP RPM/RCF HR:MIN °C Actual Value Display Rotor : JA-25.50 Speed : 0 Time : 00:00 Temp : 25°C Max Temp : 29°C Accel : MAX Decel : Setup Screen START STOP ±. ® Figure 2-2. The Control Panel Actual Value Display The actual value display (Figure 2-3) indicates the actual centrifuge operating conditions.

  • Page 36: Figure 2-4. The Setup Screen

    Description TIME • In time mode, indicates the remaining run time in hours and minutes. The TIME display begins counting down when [ START is pressed and continues counting down to 0, when deceleration begins. • In hold mode, indicates the time elapsed since [ ] was START pressed.
  • Page 37 Description Function Keys Pressing [ ] or another function key saves parameter entries. ENTER Pressed to select the rotor in use. When [ ] is pressed, the rotor ROTOR ROTOR type soft keys are displayed (JA: fixed angle rotors; JS: swinging bucket rotors;...

  • Page 38: Keypad

    Description Pressed to enter run temperature (using the keypad), from –20°C to TEMP +40°C. • The minimum allowable set temperature depends on the set speed and the rotor in use. If a temperature is entered that cannot be achieved by the rotor at the set speed, the TEMP°C field will flash. •...

  • Page 39: System Keys

    Description SYSTEM KEYS Pressing [ ], then [ ] begins the run. ([ ] must be START ENTER START START pressed within 5 seconds of pressing [ ].) This key combination ENTER can also be used to abort a deceleration process and restart the centrifuge.
  • Page 40 Operation This section contains detailed centrifuge operating procedures. A summary is provided at the start of this section. If you are an experi- enced user of this centrifuge, you can turn to the summary for a quick review of operating steps. WARNING Normal operation may involve the use of solutions and test samples that are patho-...

  • Page 41: Operation

    Operation SUMMARY OF RUN PROCEDURES For runs at other than room temperature, precool or prewarm the rotor to the required temperature before the run. MANUAL RUN Press the POWER switch to on (I). Depress the foot pedal to open the chamber door. Install the rotor according to instructions in the applicable rotor manual, then firmly close the chamber door.

  • Page 42: Programmed Run

    Operation PROGRAMMED RUN Press the POWER switch to on (I). Depress the foot pedal to open the chamber door. Install the rotor according to instructions in the applicable rotor manual, then firmly close the chamber door. Press [ ], then use the keypad to enter the program number. Check that all parameters are correct and that the door is closed.

  • Page 43: Manual Operation

    Operation Action Result CAUTION 3. Install the rotor according Do not drop the rotor onto the drive hub. The to the instructions in the drive spindle can be bent if the rotor is forced rotor manual. Make sure sideways or dropped onto the spindle hub. that the rotor is seated on Install the rotor by centering it over the hub the drive hub.

  • Page 44: Selecting A Rotor

    Operation SELECTING A ROTOR Action Result 1. Press [ The Rotor: field flashes, and rotor type abbreviation soft keys ROTOR (JA: fixed angle, JS: swinging bucket, JLA: lightweight fixed angle; JV: vertical tube rotors; JE: elutriator rotors; JCF-Z: continuous flow/ zonal) appear.
  • Page 45 Operation Entering RPM Action Result 1. Press [ The Speed: field flashes, and RPM and RCF soft keys appear. The SPEED RPM indicator illuminates in the actual value display. ROTOR ID SPEED TIME TEMP HR:MIN °C Rotor : JA-25.50 Speed : - - -- x G Time : 00:00...

  • Page 46: Entering Run Time

    Operation Action Result The RPM = - - × G field flashes. 2. Press the RCF soft key. ROTOR ID SPEED TIME TEMP RPM/RCF HR:MIN °C Rotor : JA-25.50 Speed : - - = 75 600 x G Time : 00:00 Temp : 25°C Max Temp : 29°C Accel : MAX...
  • Page 47 Operation Action Result 2. Use they keypad to enter Entered time appears in the Time: field. the required time (up to 99 hours, 59 minutes) and press [ ENTER Rotor : JA-25.50 HH:MM Speed : 25,000 Time : 00:25 HOLD Temp : 25°C Max Temp : 29°C Accel : MAX...
  • Page 48 Operation ROTOR ID SPEED TIME TEMP RPM/RCF °C rad2/sec Rotor : JA-25.50 HH:MM Speed : 25,000 1.05E03 ω2t Time : HOLD Temp : 25°C Max Temp : 29°C Accel : MAX Decel : MAX Action Result 4. Press the next function key or The centrifuge begins calculating and displaying the accumulated press [ ], then [...

  • Page 49: Entering Run Temperature

    Operation Action Result 3. Press the next function key or After you start the run, the HOLD indicator illuminates in the TIME press [ ], then [ display. The elapsed run time is displayed. The run continues until ENTER START ] is pressed.

  • Page 50: Entering Acceleration And Deceleration Rates

    Operation Action Result 5. Choose YES to make the Your selection (YES or NO) appears to the right of the Max Temp: system shut down at field. maximum temperature, or NO to allow the system to run above maximum Rotor : JA-25.50 temperature.
  • Page 51 Operation The default setting for both acceleration and deceleration is maximum (MAX) for all rotors except the JS-24.38 and the JS-24.15. SLOW is the default setting for these rotors; TIMED settings can also be used. Acceleration Action Result 1. Press [ The Accel: field flashes.

  • Page 52: Starting A Run

    Operation Action Result 2. Press the appropriate If you choose MAX, SLOW, or OFF, your selection appears in the soft key. Decel: field. If you select TIME, enter a deceleration time and press ] again or accept the 10-minute default setting. Rotor : JA-25.50 SLOW Speed : 25 000...

  • Page 53: Programmed Operation

    Operation ➠ NOTE If a diagnostic condition causes the run to stop, a diagnostic message appears and the red [ STOP indicator light flashes until the rotor comes to a complete stop. See Table 4-1 for diagnostic code explanations and troubleshooting procedures. PROGRAMMED OPERATION The centrifuge internal memory can store up to thirty programs, each containing up to two steps (each step is a set of run parameters).
  • Page 54 Operation Action Result 3. Enter run parameters (rotor, The message “Switch steps, save, or exit program mode” appears. speed, time, temperature, ➠ acceleration/deceleration NOTE settings) for step 1. Press To program a delayed start, set the time in step 1 ] when programming ENTER to the required delay length and set the speed in...

  • Page 55: Modifying A Program

    Operation Action Result 7. Press the SAVE soft key The program is saved. when programming is complete. 8. Press the EXIT soft key to exit program mode. MODIFYING A PROGRAM Action Result 1. Press the [ ] key. The Prog Recall: field flashes, and the message “Enter program number (1–30)”...

  • Page 56: Running A Program

    Operation Action Result 6. Press the SAVE soft key to Steps 1 and 2 are saved under the selected program number. save both program steps. STEP1 Rotor : JA-25.50 STEP2 Speed : 25 000 SAVE Time : 00:20 EXIT Temp : 20°C Max Temp : 24°C Shutdown? No Accel : 5...
  • Page 57 Operation Action Result 1. Turn the key to the ZONAL Open-door operation up to 3000 rpm is enabled. JCF-Z appears in the position. Rotor: field. ZONAL Rotor : JCF-Z JCF-Z Speed : 0 Time : 00:00 Temp : 25°C Max Temp : 29°C Accel : MAX Decel : MAX 2.

  • Page 58: Setting Up An Elutriation Run

    Operation Action Result WARNING 8. Open the door and unload Never try to slow or stop the rotor by hand. the rotor. ➠ NOTE 9. Press [ ] to bring the Before another zonal run can be started, you STOP rotor to a complete stop.
  • Page 59 Operation Action Result 1. Install the rotor. 2. Set up sample and buffer reservoirs and tubing lines as described in the rotor manual. Route tubing lines through the elutriator port holes at the left side of the door seal. Close the centrifuge door. 3.
  • Page 60 Operation Action Result 7. When the rotor speed has stabilized, look through the port in the centrifuge door and turn the DELAY knob DELAY Delay until the elutriation chamber Control ELUTRIATOR Knob in the rotor is synchronized with the strobe. (The chamber will appear to Sample can now be injected into the lines.

  • Page 61: Troubleshooting

    Table 4-1 to determine the nature of the condition and any recom- mended actions. If a problem persists after you have performed the recommended action, call your Beckman Coulter Field Service representative. To help the field service representative diagnose and correct the problem, gather as much information about the...

  • Page 62: Table 4-1. Diagnostic Message Chart

    If this does not work, call Beckman to manual of run Coulter Field Service. Door problem, refer to manual In the U.S. call 1-800-742-2345. Outside the United States, contact your local Beckman Coulter office. Continued —...
  • Page 63 System shuts down Call Beckman Coulter Field System error the system control soft- Service. ware, EPROM, or RAM * In the U.S. call 1-800-742-2345. Outside the United States, contact your local Beckman Coulter office. † Friction Reduction System. Continued —...
  • Page 64 Troubleshooting Table 4-1. Diagnostic Message Chart (continued) Diagnostic Number/ Message Problem Result Recommended Action a) a run is in progress The centrifuge does not Press [ ] (green light on STOP Speed error (at speed or accelerate, and the H4 ] key will turn off), then START accelerating)

  • Page 65: Accessing The Rotor In Case Of Power Failure

    Troubleshooting ACCESSING THE ROTOR IN CASE OF POWER FAILURE WARNING Any maintenance procedure requiring removal of a panel exposes the operator to the possibility of electrical shock and/or mechanical injury. Therefore, turn the power off (O) and disconnect the instrument from the main power source, and refer such main- tenance to qualified service personnel.

  • Page 66: Figure 4-1. Emergency Door Release Latch Access

    Troubleshooting Figure 4-1. Emergency Door Release Latch Access Action Result 1. Turn the power switch to off (O) and unplug the power cord from the main source. 2. Insert a 4-mm or -in. The latch disengages. Allen wrench straight through one of the holes (either one) and turn the wrench to the left (counter- clockwise) about one-half...

  • Page 67: Figure 4-2. Manual Door Release

    Troubleshooting Action Result 4. Lift the front panel up and off An inner front panel extends about halfway down from the centrifuge the centrifuge; set it aside. top (see Figure 4-2). Below this inner panel is a black manual door release interlock switch (see Figure 4-2).
  • Page 68 Troubleshooting Action Result 7. To vent the chamber vacuum, The vacuum will be completely vented several seconds after the hose grasp the red rubber hose and is detached. pull it up until it comes off ➠ of the pump fitting (see NOTE Figure 4-3).

  • Page 69: Figure 4-3. Venting The Chamber Vacuum

    Troubleshooting Grasp red hose and pull up. Do not disconnect the tubing lines tied to the red hose. Vacuum Pump Red hose removed from fitting Replace red hose Figure 4-3. Venting the Chamber Vacuum...

  • Page 70: Jcf-Z Rotor Identification

    Troubleshooting To replace the panel on the centrifuge, Action Result 1. Holding the panel at the top (with the instrument label facing out), insert the three grooved brackets on the bottom inside of the door over the front panel retaining strip (see Figure 4-4).
  • Page 71 If the JCF-Z rotor is misidentified, the run speed will be limited to the maximum speed for the identified rotor. (The maximum speed for the JCF-Z rotor is 20 000 rpm.) In the U.S. call 1-800-742-2345. Outside the United States, contact your local Beckman Coulter office. 4-11...

  • Page 72: Care And Maintenance

    WARNING Do not use alcohol or other flammable substances in or near operating centrifuges. In the U.S., call 1-800-742-2345. Outside the U.S., contact your local Beckman Coulter office.

  • Page 73: Maintenance

    Care and Maintenance MAINTENANCE Perform the following procedures regularly to ensure continued performance and long service life of the centrifuge. • Inspect the interior of the centrifuge chamber for accumulations of sample, dust, or glass particles from broken sample tubes. Clean as required (see , below).

  • Page 74: Decontamination

    IN-175 for chemical resistances of centrifuge and acces- sory materials. While Beckman Coulter has tested these methods and found that they do not damage the centrifuge, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 75: Replacing The Air Filter

    OFF (O) position. Reset the circuit breaker by turning the power switch back to the ON (I) position. If it trips again immedi- ately, do not reset it. Call Beckman Coulter Field Service. CAUTION Repeated attempts to reset the circuit breaker can cause substantial damage to electrical and electronic components.

  • Page 76: Storage And Transport

    Before returning a centrifuge or accessory for any reason, prior permission (a Returned Goods authorization form) must be obtained from Beckman Coulter, Inc. Contact your local Beckman Coulter office to obtain the RGA form and for packaging and shipping instructions.

  • Page 77: Supply List

    Care and Maintenance SUPPLY LIST Refer to Beckman Coulter High Performance, High Speed, High Capacity Rotors, Tubes & Accessories (BR-8102, available at www.beckmancoulter.com) and the applicable rotor manual for materials and supplies needed for rotors. REPLACEMENT PARTS AND SUPPLIES Air filter..........885218 Beckman Solution 555™...

  • Page 78: Appendix

    APPENDIX Temperature Calibration Procedure The Avanti J-25I centrifuge specification for temperature control is ±2°C of the set temperature. That means that your sample will stay within 2°C of set temperature at all times, after rotor and system equilibration, described below. (During transient conditions, such as acceleration and deceleration, the rotor temperature may be outside this range.) The following procedure is provided for those cases in which temperature control within ±1°C is required.
  • Page 79 Temperature Calibration Procedure 5. After at least 1 hour, measure the temperature of the buffer or water using a thermometer or thermocouple. 6. If the measured and set temperatures are different, note how many degrees different the measurements are and adjust the set tempera- ture up or down that number of degrees.
  • Page 80 Beckman Coulter, vice for a reasonable period of time, Beckman Coulter will or unless such repair in the sole opinion of Beckman Coulter repair or, at its election, replace such component or accessory.
  • Page 81 Telephone: (27) 11-805-2014/5 Fax: (27) 11-805-4120 e-mail: beckman@intekom.co.za Beckman Coulter, Inc. • 4300 N. Harbor Boulevard, Box 3100 • Fullerton, California 92834-3100 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com • Telex: 678413 • Fax: 1-800-643-4366 ©2007 Beckman Coulter, Inc. Printed on recycled paper...

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