Beckman Coulter JA-25.50 Instructions For Use Manual
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Beckman Coulter JA-25.50 Instructions For Use Manual

Fixed-angle rotor
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PN J-TB-056FD
September 2016
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.
Instructions For Use
JA-25.50
Fixed-Angle Rotor
For Use in Beckman Coulter
J2 and Avanti J Series Centrifuges

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Summary of Contents for Beckman Coulter JA-25.50

  • Page 1 Instructions For Use JA-25.50 Fixed-Angle Rotor For Use in Beckman Coulter J2 and Avanti J Series Centrifuges PN J-TB-056FD September 2016 Beckman Coulter, Inc. 250 S. Kraemer Blvd. Brea, CA 92821 U.S.A.
  • Page 2 Beckman Coulter, Inc. Avanti, Beckman Coulter, and the stylized logo are trademarks of Beckman Coulter, Inc. and are registered in the USPTO. All other trademarks, service marks, products, or services are trademarks or registered trademarks of their respective holders.
  • Page 3 This rotor was developed, manufactured, and tested for safety and reliability as part of a Beckman Coulter centrifuge/rotor system. Its safety or reliability cannot be assured if used in a centrifuge not of Beckman Coulter’s manufacture or in a Beckman Coulter centrifuge that has been modified without Beckman Coulter’s approval.
  • Page 4 Do not centrifuge such materials in nor handle or store them near the centrifuge. Although rotor components and accessories made by other manufacturers may fit in the JA-25.50 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’...
  • Page 5 Safety Notice, iii Alerts for Warning, Caution, Important, and Note, iii Safety Information for the JA-25.50 Rotor, iii JA-25.50 Fixed-Angle Rotor, 1 Specifications, 1 Description, 2 Preparation and Use, 4 Care and Maintenance, 14 Returning a Rotor, 18 Supply List, 19 Beckman Coulter, Inc.
  • Page 6 The JA-25.50 Fixed-Angle Rotor, 3 Fluid-Containment Annulus and Finger-Grip Grooves, 4 Typical Examples of Arranging Tubes in the Rotor, 5...
  • Page 7 Temperature Compensation Settings for Analog- and Microprocessor- Controlled Centrifuges Temperature Compensation Settings for the Model J2-HC Analog Centrifuge. Available Tubes and Bottles for the JA-25.50 Rotor, 12 Relative Centrifugal Fields for the JA-25.50 Rotor, 15...

  • Page 8: Controlled

    JA-25.50 Fixed-Angle Rotor Specifications Maximum speed..........25,000 RPM 34°...

  • Page 9: Description

    Beckman Coulter JA-25.50 rotors are manufactured in a facility that maintains certifications to both ISO 9001:2008 and ISO 13485:2003. They are for use with the specified Beckman Coulter centrifuges. The JA-25.50 fixed-angle rotor is designed to hold up to eight 50-mL tubes at a 34-degree angle (see Figure 1).

  • Page 10: The Ja-25.50 Fixed-Angle Rotor

    Beckman Coulter centrifuge when used and maintained as instructed. The JA-25.50 rotor has a patented fluid-containment annulus, located below the O-ring sealing surface (see Figure 2).

  • Page 11: Preparation And Use

    Beckman Coulter. Use of other manufacturers’ components or accessories in the JA-25.50 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. Only the components and accessories listed in this publication should be used in this rotor.
  • Page 12 If fluid containment is required, use capped tubes or bottles. It is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage. If you are using an Avanti J series centrifuge, select the JA-25.50 rotor. PN J-TB-056FD...
  • Page 13 JA-25.50 Fixed-Angle Rotor Preparation and Use 25.5 If you are using a microprocessor-controlled J2 series centrifuge, enter rotor code Refer to your centrifuge instruction manual for additional information. For additional operating information, see the following: • Times, page 13, for using k factors to adjust run durations.
  • Page 14 Temperature To ensure that the JA-25.50 rotor reaches the required temperature during the run, follow the appropriate instructions below for the model of J centrifuge being used.
  • Page 15 JA-25.50 Fixed-Angle Rotor Preparation and Use J2 Series Centrifuges Enter the required run temperature and the appropriate temperature compensation units (see Table 1 Table 2) on the centrifuge control panel as follows. Table 1 Temperature Compensation Settings for Analog- and Microprocessor-Controlled Centrifuges Rotor Required Sample Temperature (°C)
  • Page 16 JA-25.50 Fixed-Angle Rotor Preparation and Use Microprocessor-Controlled J2 Series Centrifuges (Models J2-MI and J2-MC) Press the TEMP key on the centrifuge control panel and then use the keypad to enter the sample temperature. Find the compensation value in Table 1 that corresponds with the set temperature and run speed.
  • Page 17 JA-25.50 Fixed-Angle Rotor Preparation and Use WARNING Operator error or tube failure may generate aerosols. Toxic, pathogenic, or other hazardous materials must not be run in this rotor unless all appropriate safety precautions are taken. Also see the Safety Notice at the beginning of this manual.
  • Page 18 Remove the lid and set it aside. Lift the rotor straight up off the drive hub to avoid bending or damaging the hub. Tubes and Bottles The JA-25.50 rotor uses the tubes and bottles listed in Table 3. Be sure to use only those items listed, and to observe the maximum speed limits and fill volumes shown.
  • Page 19 JA-25.50 Fixed-Angle Rotor Preparation and Use Table 3 Available Tubes and Bottles for the JA-25.50 Rotor Tube or Bottle Required Accessory Speed Max. Fill RCF/ Dimensions Part Volume Part k factor and Volume Description Number (mL) Description Number 24  104 mm...
  • Page 20 Use only the items listed here and observe fill volumes and maximum run speeds. b. Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of manufacturing variances no guarantee of performance or fit is expressed or implied.

  • Page 21: Care And Maintenance

    If the drive pins appear damaged, contact Beckman Coulter Field Service. * In the United States call 1-800-742-3485; outside the U.S. contact your local Beckman Coulter office or visit us on the web at www.beckmancoulter.com PN J-TB-056FD...
  • Page 22 JA-25.50 Fixed-Angle Rotor Care and Maintenance Table 4 Relative Centrifugal Fields for the JA-25.50 Rotor Relative Centrifugal Field (g) At r At r At r Rotor Speed (RPM) (108 mm) (73.2mm) (38.5 mm) 25,000 75,600 51,200 27,000 23,000 64,000 43,400...
  • Page 23 Replace O-rings about twice a year or whenever worn or damaged. Refer to Chemical Resistances (publication IN-175) for the chemical compatabilities of rotor and accessory materials. Your Beckman Coulter representative provides contact with the Field Rotor Inspection Program and the rotor repair center. Cleaning Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred.
  • Page 24 If the rotor (and/or accessories) becomes contaminated with radioactive material, it should be decontaminated using a solution that will not damage the anodized surfaces. Beckman Coulter has tested a number of solutions and found two that do not harm anodized aluminum: RadCon Surface †...

  • Page 25: Returning A Rotor

    Ethanol is a flammability hazard. Do not use it in or near operating centrifuges. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfection is a concern, consult your laboratory safety officer regarding proper methods to use.

  • Page 26: Supply List

    NOTE Publications referenced in this manual can be obtained at www.beckmancoulter.com, by calling Beckman Coulter at 1-800-742-2345 in the United States, or by contacting your local Beckman Coulter office. See the High Performance, High Speed, High Capacity Rotors, Tubes & Accessories catalog (BR-8102) for detailed information on ordering parts and supplies.
  • Page 27 JA-25.50 Fixed-Angle Rotor Supply List Description Part Number Solution 555 (1 qt) 339555 Spinkote lubricant (2 oz) 306812 PN J-TB-056FD...
  • Page 28 A defective rotor will be replaced by Beckman Coulter, Inc. at its then current list price less a credit based upon the age of the rotor (years since date of purchase). The Buyer shall not receive credit until the claimed defective rotor is returned to Beckman Coulter’s Indianapolis, Indiana facility or...
  • Page 29 Beckman Coulter, Inc. J Series Rotor Warranty Warranty-2 PN J-TB-056FD...
  • Page 31 PN IN-192 Chemical Resistances for Beckman Coulter Centrifugation Products PN IN-175 High Performance, High Speed, High Capacaity Rotors, Tubes, & Accessories PN BR-1802 Available in hard copy by request. Available at www.beckmancoulter.com www.beckmancoulter.com © 2016 Beckman Coulter, Inc. All Rights Reserved...

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