Related Manuals for Beckman Coulter JA-25.50
Summary of Contents for Beckman Coulter JA-25.50
- Page 1 J-TB-056F October 2007 JA-25.50 Fixed Angle Rotor Used In Beckman Coulter J2 and ® Avanti J Series Centrifuges © 2007 Beckman Coulter, Inc. Printed in the U.S.A.
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Page 2: Safety Notice
Although rotor components and accessories made by other manufacturers may fit in the JA-25.50 rotor, their safety in this rotor cannot be ascertained by Beckman Coulter. Use of other manufacturers’ components or accessories in the JA-25.50 rotor may void the rotor warranty and should be prohibited by your laboratory safety officer. -
Page 3: Ja-25.50 Fixed Angle Rotor
JA-25.50 Rotor JA-25.50 FIXED ANGLE ROTOR SPECIFICATIONS Maximum speed ........25 000 rpm* †... - Page 4 This rotor has been manufactured in a registered ISO 9001 or 13485 facility for use with the specified Beckman Coulter centrifuges. The JA-25.50 fixed angle rotor, rated for 25 000 rpm, is designed Daisy Knob to hold up to eight 50-mL tubes at a 34-degree angle (see Figure 1).
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Page 5: Preparation And Use
Figure 2. Fluid-Containment Annulus and Finger-Grip Grooves PREPARATION AND USE Specific information about the JA-25.50 rotor is given here. Use the Rotors and Tubes for Beckman Coulter J Series Centrifuges manual (publication JR-IM) and the centrifuge instruction manual along with this manual for complete rotor and accessory operation. -
Page 6: Prerun Safety Checks
JA-25.50 Rotor PRERUN SAFETY CHECKS Read the Safety Notice page at the front of this manual before using the rotor. 1. Make sure that the rotor, lid, and all tubes or bottles and accesso- ries are clean and show no signs of corrosion or cracking. -
Page 7: Operation
• If fluid containment is required, use capped tubes or bottles. It is strongly recommended that all containers carrying physiological fluids be capped to prevent leakage. • If you are using an Avanti J series centrifuge, select the JA-25.50 rotor. • If you are using a microprocessor-controlled J2 series centrifuge, enter rotor code 25.5. - Page 8 TEMPERATURE To ensure that the JA-25.50 rotor reaches the required temperature during the run, follow the appropriate instructions below for the model of J centrifuge being used. Avanti J Series Centrifuges Enter the run temperature according to the instructions in your centrifuge instruction manual.
- Page 9 JA-25.50 Rotor Table 1. Temperature Compensation Settings for Analog- and Microprocessor-Controlled Centrifuges (Models J2-HS, J2-MI, and J2-MC). Interpolate if intermediate values are required. Required Sample Temperature (°C) Rotor Speed (rpm) –20 –10 21 000 –7 –5 –6 –5 –9 18 000 –5...
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Page 10: Run Procedure
JA-25.50 Rotor Microprocessor-Controlled J2 Series Centrifuges (Models J2-MI and J2-MC) 1. Press the [ ] key on the centrifuge control panel and then use TEMP the keypad to enter the sample temperature. 2. Find the compensation value in Table 1 that corresponds with the set temperature and run speed. -
Page 11: Removal And Sample Recovery
JA-25.50 Rotor CAUTION Make sure that the rotor is properly seated on and securely fastened to the drive hub before each run. REMOVAL AND SAMPLE RECOVERY CAUTION If disassembly reveals evidence of leakage, you should assume that some fluid escaped the rotor. -
Page 12: Tubes And Bottles
TUBES AND BOTTLES The JA-25.50 rotor uses the tubes and bottles listed in Table 3. Be sure to use only those items listed, and to observe the maximum speed limits and fill volumes shown. (Maximum fill volume is the maximum amount that can be centrifuged in the container listed.) - Page 13 870331 a Maximum speeds listed are guidelines only. These speeds have been achieved in reliability tests at Beckman Coulter, but because of manufacturing variances no guarantee of performance or fit is expressed or implied. Polypropylene, package of 25.
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Page 14: Run Times
JA-25.50 Rotor Polycarbonate and Polyallomer Bottles Capped polycarbonate and polyallomer bottles may be centrifuged completely filled, or partially filled (not less than half full). Containers placed in opposing positions in the rotor must be filled to the same level. RUN TIMES To pellet particles of known sedimentation coefficient s, calculate the run time t using the k factor (395) of the rotor. - Page 15 JA-25.50 Rotor Table 4. Relative Centrifugal Fields for the JA-25.50 Rotor. Entries in this table are calculated from the formula RCF = 1.12 r (RPM/1000) and then rounded to three significant digits. Relative Centrifugal Field (× g) Rotor At r...
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Page 16: Care And Maintenance
O-rings about twice a year or whenever worn or damaged. Refer to Appendix A in Rotors and Tubes for chemical compatibili- ties of rotor and accessory materials. Your Beckman Coulter repre- sentative provides contact with the Field Rotor Inspection Program... - Page 17 JA-25.50 Rotor CLEANING Wash the rotor and rotor components immediately if salts or other corrosive materials are used or if spillage has occurred. Do not allow corrosive materials to dry on the rotor. Rotor Cleaning Kit (339558) Under normal use, wash the rotor frequently (at least weekly) to prevent buildup of residues.
- Page 18 Use it only when necessary and remove it promptly from surfaces. While Beckman Coulter has tested these methods and found that they do not damage components, no guarantee of decontamination is expressed or implied. Consult your laboratory safety officer regarding the proper decontamination methods to use.
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Page 19: Sterilization And Disinfection
Use the minimum immersion time for each solution, per laboratory standards. While Beckman Coulter has tested these methods and found that they do not damage the rotor or components, no guarantee of sterility or disinfection is expressed or implied. When sterilization or disinfec- tion is a concern, consult your laboratory safety officer regarding proper methods to use. -
Page 20: Returning A Rotor
Before returning a rotor or accessory for any reason, prior permission (a Returned Goods Authorization form) must be obtained from Beckman Coulter, Inc. This RGA form, which may be obtained from your local Beckman Coulter sales office, should contain the following information: •... -
Page 21: Supply List
Beckman Coulter office. Contact Beckman Coulter Sales (1-800-742-2345 in the United States), or your local Beckman Coulter office, or see the High Perfor- mance, High Speed, High Capacity Rotors, Tubes & Accessories catalog (BR-8102) for detailed information on ordering parts and supplies. - Page 22 CONDITIONS clause of the Beckman Coulter, Inc., terms and conditions of sale in effect at the time of sale, Beckman Coulter, Inc. agrees 1. Except as otherwise specifically provided herein, this war- to correct either by repair, or, at its election, by replacement, ranty covers the rotor only and Beckman Coulter, Inc.
- Page 23 Telephone: (27) 11-805-2014/5 Fax: (27) 11-805-4120 e-mail: beckman@intekom.co.za Beckman Coulter, Inc. • 4300 N. Harbor Boulevard, Box 3100 • Fullerton, California 92834-3100 Sales and Service: 1-800-742-2345 • Internet: www.beckmancoulter.com • Telex: 678413 • Fax: 1-800-643-4366 ©2007 Beckman Coulter, Inc. Printed on recycled paper...
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