Prerun Safety Checks; Rotor Preparation - Beckman Coulter JA-25.50 Manual

JA-25.50 Rotor

PRERUN SAFETY CHECKS

ROTOR PREPARATION

6
Read the Safety Notice page at the front of this manual before using
the rotor.
1. Make sure that the rotor, lid, and all tubes or bottles and accesso-
ries are clean and show no signs of corrosion or cracking.
2. Verify that the bottles or tubes being used are listed in Table 3.
3. Refer to Appendix A in Rotors and Tubes for the chemical
compatibilities of all materials used.
For runs at other than room temperature, refrigerate or warm the
rotor beforehand for fast equilibration.
1. Be sure that metal threads in the rotor are clean and lightly but
evenly lubricated with Spinkote™ lubricant (306812). Also ensure
that O-rings are lightly but evenly coated with silicone vacuum
grease.
2. Load the filled containers symmetrically into the rotor. (Refer
to
TUBES AND BOTTLES
containers.) If fewer than eight tubes are being run, they must
be arranged symmetrically in the rotor (see Figure 3). Opposing
tubes must be filled to the same level with liquid of the same
density.
Figure 3. Typical Examples of Arranging Tubes in the Rotor.
Two, four, six, or eight tubes can be centrifuged per run,
if they are arranged in the rotor as shown.
on page 12 for information about