Beckman Coulter J-Lite JLA 16.250 Instruction Manual page 19

RUN SPEEDS
SPEED
For centrifugation below maximum speed, estimate the run time
required by adjusting the k factor as follows:
k
adj
Run times can also be estimated from data established in prior
experiments using a different rotor if the k factor of the previous rotor
is known. For any two rotors, a and b:
Where the k factors have been adjusted for the actual run speed used.
For more information on k factors, see Use of k Factor for
Estimating Run Times from Previously Established Run Conditions
(publication DS-719).
The centrifugal force at a given radius in a rotor is a function of
run speed. Comparisons of forces between different rotors are made
by comparing the rotors' relative centrifugal fields (RCF). When
rotational speed is adjusted so that identical samples are subjected
to the same RCF in two different rotors, the samples are subjected
to the same force. The RCF at a number of rotor speeds is provided
in Table 4.
k
t = - -
s
2
maximum speed
 
= k ---------------------------------------- -
 
actual run speed
t k
a a
----- = ------
t k
b b
J-Lite JLA-16.250 Rotor
(3)
(4)
(5)
19