tecan cavro xlp 6000 Operating Manual page 90

4 - Setting Up the XLP 6000 for Your Application
Optimizing XLP 6000 Performance
4 - 6
Possible errors:
– No response. Check for loose or incorrectly connected cables, or
connection to the wrong computer COM port. Retry.
6 Initialize pump and set initialization speed.
The following information assumes that your input tubing connects to the right
valve port. If your input tubing connects to the left valve port, exchange [Y] for
all instances of [Z] in the following commands.
– Send the command [ZR] to initialize the pump. Successful initialization will
move the syringe plunger to the position "0" (fully dispensed) and return a
"Ready" status.
Possible errors:
– Error 1 (initialization error). Check for tubing blockage and reinitialize. If
you are using very narrow I.D. tubing or pumping a viscous fluid, the
initialization speed may need to be reduced.
– This is accomplished (only if using a 1 mL or larger syringe) by sending
the command [Z16R] (initializes at full-force, reduced speed). Repeat with
decreasing initialization speed (increase "Z_" value) until the pump
successfully initializes.
7 Prime the syringe.
– Send the command [IA6000OA0R] to pull fluid through the valve input
position and into the syringe.
– Inspect the pump tubing and syringe for bubbles and re-prime until all
bubbles are completely gone.
If bubbles remain after several priming strokes, disassemble the syringe
and clean it with alcohol. Also check to ensure the fittings are tight and the
syringe is tight within the Teflon fitting.
– Re-prime.
Possible errors:
– Error 9 (plunger overload). See step 8.
8 Check aspirate/dispense.
Send the command [IA6000OA0R] to aspirate a full syringe stroke (6000
increments) from input and dispense it to output. Successful execution will
move the syringe plunger to position "6000" then back to "0," then it will return
a "Ready" status.
Possible errors:
– Error 9 (plunger overload). The stepper motor is unable to move the
syringe plunger, probably because of excessive backpressure caused by
excessive flowrate, narrow tubing I.D., or valve or tubing blockage. Note
whether the error occurred during aspiration or dispensing. To differentiate
between blockage and flowrate limitation, reduce syringe plunger speed
by sending the command [S12IA3000OA0R]. Repeat with decreasing
plunger speed (increase "S_" value) until the pump aspirates and
dispenses successfully.
9 Set start speed and top speed.
The XLP 6000 plunger speed can be controlled from 1.2 seconds per stroke
to 160 minutes per stroke (top speed) using the [S] or [V] commands. (The [V]
command allows a slightly larger speed range.) As a general rule, aspiration
Cavro® XLP 6000 Modular Syringe Pump Operating Manual, 734237-C, en