tecan cavro xlp 6000 Operating Manual page 86

4 - Setting Up the XLP 6000 for Your Application
Glossary
4 - 2
carryover
Contamination of a volume of fluid by residual fluid from a previous aspiration
or dispense. Carryover causes variability in final volume and concentration.
cavitation
Formation of air bubbles due to rapid pressure changes. Often caused by
aspirating fluid into the syringe too quickly.
dilution effect
Reduction in sample or reagent concentration, caused by contact with system
fluid or residual fluid from a previous aspiration or dispense.
I.D. ("inner diameter")
Diameter of the constraining wall of a fluid path.
priming
Completely filling the pump tubing and syringe with bubble-free fluid to allow
sustained, reproducible pumping action. The air in an unprimed line acts as a
spring, adversely affecting accuracy and precision.
reagent tubing
Connects the valve input port (1/4-28 thread or M6 fitting) to a reagent source.
Reagent tubing is used to fill the pump syringe; it tends to have a larger I.D.
than aspirate/dispense tubing, and a blunt-cut end which extends into the
reagent.
system fluid
A fluid used to prime the pump system that does not act as sample or reagent.
Typically the system fluid is de-ionized water or a wash buffer and is isolated
from sample or reagent fluid by an air gap to avoid intermixing.
syringe speed profile
Typically, the syringe plunger begins moving slowly, then ramps up to top
speed. This allows the plunger to start moving gradually, without overloading
the motor, and still provide maximum flowrate. The syringe plunger stops by
ramping down in speed. This results in the most reproducible fluid breakoff for
accurate dispensing.
Cavro® XLP 6000 Modular Syringe Pump Operating Manual, 734237-C, en