Page 1 Operation Guide SyriXus Syringe Pumps Installation and Operation Guide Part #69-1263-358 Copyright © 2022. All rights reserved, Teledyne ISCO Rev. - April, 2022 Use and Disclosure of Data: Information contained herein is classified as EAR99 under the U.S. Export Administration Regulations. Export, reexport or diversion contrary to U.S. law is prohibited.
Page 2 This manual may help in diagnosing and repairing the malfunction. If a problem persists, call or e-mail Teledyne ISCO technical support for assistance. Refer to Section 9.3 "Customer Service Department". Simple difficulties can often be diagnosed over the phone. For faster service, please have your serial number ready.
Page 3 Warnings and Cautions Preface Warnings and Cautions The lightning flash and arrowhead within the triangle is a warning sign alerting you to “dangerous voltage” inside the product. The exclamation point within the triangle is a warning sign alerting you to important instructions in this manual.
Page 4 Advertencias y Precauciones Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie darauf aufmerksam macht, daß wichtige Anleitungen zu diesem Handbuch gehören. Advertencias y Precauciones Esta señal alerta sobre la presencia de alto voltaje en el interior del producto. Esta señal le advierte sobre la importancia de las instrucciones del manual que acom- pañan a este producto.
Page 5 General Product Warnings and Cautions “For a forhindre skade på instrumentet eller operatøren er det nød- vendig at brukeren har full forståelse for det engelske sprak og tekniske uttrykk Ellers må brukeren få opplæring av en person, som kan engelsk, for instrumentet tas i bruk.” “För att förhindra skade på...
Page 6 Teledyne ISCO SyriXus Syringe Pump Safety Note When Using a Flammable Fluid The Teledyne ISCO syringe pumps must be placed within a properly operating vent hood (fume cup- board), when using any flammable liquid at elevated temperatures, or ethane or any other flam- mable gas.
Page 7 Les pompes seringues de la série SyriXus Teledyne ISCO utilisent des moteurs à charbon. Des modi- fications mineures apportées aux pompes peuvent les rendre plus sûres, en particulier dans les rares cas de défaillance catastrophique du joint de piston.
Page 8 Supercritical Fluid Extraction Laboratory Staff - Warning to Users Supercritical Fluid Extraction Laboratory Staff - Warning to Users WARNING: PLEASE READ At the request of our Supercritical Fluid Extraction laboratory staff, we want our cus- tomers to be aware of the potential hazards involved with supercritical fluid extraction. Oxidizing gases, such as nitrous oxide, in contact with organic matrices or flammable modifiers, can detonate under certain conditions.
Page 9 In all cases, when using Teledyne ISCO instrumentation, prudence and common sense must be used. WARNING: Pinch point.
Page 10 Instrument Use Warnings and Cautions ATTENTION: Risque de pincement. Ce symbole vous avertit que les mains ou les doigts recevront une blessure sérieuse si vous les mettez entre les éléments en mouvement du mécanisme près de ce symbole. ATTENTION: Éviter les usages hasardeux! Si vous utilisez cet instrument d’une manière autre que celles qui sont specifiées dans ce manuel, la pro- tection fournie par l’instrument peut être affaiblie;...
Page 11 Commonly Ordered Replacement Parts for the SyriXus Series Syringe Pumps Commonly Ordered Replacement Parts for the SyriXus Series Syringe Pumps Part Model Description Cylinder Seals General 0-1379 bar 202-9096-08 260x 0-655.0 bar 202-9091-06 500x 0-344.7 bar 202-9091-56 1000x 0-137.8 bar upper seal 202-9990-25 1000x 0-137.8 bar lower seal...
Page 12 Pre-Assembled Tubing Assemblies for SyriXus Series Syringe Pumps Intentionally Left Blank EAR99 Technology Subject to Restrictions Contained on the Cover Page...
Page 13: Table Of Contents
SyriXus Series Pumps Installation and Operation Guide Table of Contents Section 1 Introduction 1.1 About This Manual ..........1-1 1.2 Specifications.
Page 14 3.5.3 External Control ..........3-8 3.5.4 Multiple Pumps .
Page 15 4.6 Program Conclusion ..........4-11 Section 5 Continuous Flow Introduction, Installation, and Opera- tion 5.1 Introduction .
Page 16 8.7.1 DASNET ........... . . 8-7 8.7.2 Universal Driver from Teledyne ISCO ......8-8 8.8 Serial Commands for the SyriXus Series Pumps .
Page 17 2-2 SyriXus 65x Fitting ........
Page 18 5-6 Keystrokes to set up constant flow ........5-15 5-7 Keystrokes to set up constant pressure .
Page 19 2-4 Temp. Control Jacket Packages ........2-12 2-5 Wetted Materials in SyriXus Series Pumps .
Page 20 Intentionally Left Blank EAR99 Technology Subject to Restrictions Contained on the Cover Page TOC-VIII...
Page 21: About This Manual
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction 1.1 About This ® The 260x pumps have 1/8” standard Valco ports. Pipe thread fittings Manual of 1/4” diameter are used for the 1000x, 3/8” diameter for the 500xv, and 1/8” diameter for the 500x. The 65x uses AE F250C high pressure fittings.
Page 22 SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-1 65x Technical Specifications CYLINDER CAPACITY 67.97 mL POWER REQUIREMENTS 100 ± 10 VAC, 1.5 A maximum Factory Set 117 ± 12 VAC, 1.5 A maximum (Mains voltage line cord is a “Disconnect 234 ±...
Page 23 SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-2 260x Technical Specifications CYLINDER CAPACITY 266.05 mL POWER REQUIREMENTS 100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum Factory Set (Mains voltage line cord is a 234 ±...
Page 24 SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-3 500x Technical Specifications CYLINDER CAPACITY 507.38 mL POWER REQUIREMENTS 100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum Factory Set (Mains voltage line cord is a 234 ±...
Page 25: 500Xv Technical Specifications
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-4 500xv Technical Specifications CYLINDER CAPACITY 507.38 mL POWER REQUIREMENTS 100 ± 10 VAC, 5A maximum 117 ± 12 VAC, 5A maximum Factory Set (Mains voltage line cord is a 234 ±...
Page 26: 1000X Technical Specifications
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-5 1000x Technical Specifications CYLINDER CAPACITY 1015.0 mL POWER REQUIREMENTS 100 ± 10 VAC, 1.5 A maximum 117 ± 12 VAC, 1.5 A maximum Factory Set (Mains voltage line cord is a 234 ±...
Page 27: 260X Flow Rate Range
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction 260x Flow Rate Range 100 125 150 175 200 225 250 275 300 325 350 375 400 425 450 475 525 550 575 600 625 650 675 Pressure (bar) Flow Rate (mL/min) Max.
Page 28: Unpacking
If there is any shipping damage, file a claim with the delivering carrier immediately. Compare the contents of the boxes with the enclosed packing slip. If there are shortages, contact Teledyne ISCO immediately using the information in 9.3 "Customer Service Department". 1.4 Controls and The pump controller regulates all pumping functions.
Page 29: Pump Controller Front Panel Label
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Figure 1-4 Pump controller key functions Table 1-6 Pump Controller Front Panel Label Figure 1-4 # Connector Description On/Standby switch Turns instrument OFF and ON. (Does not disconnect power.) Programming keypad Used to program controller.
Page 30: Pump Controller Key Functions
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-7 Pump Controller Key Functions Description ON/STANDBY Two-position toggle switch turns controller on and activates drive motor to maintain position. Standby disables the drive motor and halts the controlling processor. A, B, C, D Softkeys;...
Page 31: Pump Controller Rear Panel Connectors
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction NOTE For more information on the keypad, refer to Section 3.8 "Front Panel Keys". Figure 1-5 Pump controller rear panel connectors Table 1-8 Pump Controller Rear Panel Connectors Figure 1-5 # Connector Description PUMP D...
Page 32: Pump Controller Rear Panel Connectors
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Table 1-8 Pump Controller Rear Panel Connectors (Continued) Figure 1-5 # Connector Description RS-232/RS-485 This serial port connector may be used with an RS-232 or RS-485 cable to place the pump under remote control. Refer to Section 8 "Serial Interface"...
Page 33: Pump Rear Panel Connectors
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Figure 1-6 Pump rear panel connectors Table 1-9 Pump Rear Panel Connectors Figure 1-6 # Connector Description Pressure outputs Two red binding post/banana jacks providing pressure output voltages. Pump Left Jack VDC/psi Right Jack VDC/psi 1.0 V/2000 psi 0.1 V/2000 psi...
Page 34: Pump Front Panel Controls
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Figure 1-7 Pump front panel controls Table 1-10 Pump Front Panel Figure 1-7 # Connector Description Mains power switch Disconnects power from the pump circuits for setup changes, such as connecting the controller.
Page 35: Electrical Connections
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction 1.5 Electrical The pump controller may be placed on top of the pump, or wherever Connections safety and convenience dictate. Power is supplied to the pump con- troller through the control cable. Caution All connections between the pump and controller should be made BEFORE the pump is connected to mains power.
Page 36: Cabling
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction 1.5.1 Cabling For all systems, connect the control cable of the pump module (Pump A for multiple-pump systems) to the connector labeled P on the rear panel of the controller. Subsequent pumps in mul- tiple-pump systems can be connected to controller connectors P C, and P D.
Page 37: Syringe Pump Cylinder Cap: Attach To Pressure Transducer Connector On Back Of Pump
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction Transducer Cable, 6 ft (1.8 m) Transducer Cap Assembly for the 65x Figure 1-8 Syringe pump cylinder cap: Attach to pressure transducer connector on back of pump. Caution The pressure transducer cable length is always 6 feet (1.8 m). Never attempt to extend the length of this cable.
Page 38: Preliminary Checkout
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction 1.6 Preliminary After the electrical connections have been completed, follow this brief Checkout test of the pump’s operation: NOTE Preliminary checkout of the pump is performed without fluid in the pump.
Page 39: Menu 1
SyriXus Series Pumps Installation and Operation Guide Section 1 Introduction 4. Press to display Menu 1. MENU 1. UNITS 4. POWER FAILURE [STOP] 2. SELECT PUMP 5. SYSTEM RESET 3. REFILL 6. DISPLAY CONTRAST MORE RETURN Figure 1-11 Menu 1 5.
Page 40: Four Pump Stop Menu
PUMP A Figure 1-13 Four pump stop menu If you encounter any problems during the preliminary checkout, please contact the Teledyne ISCO Customer Service Department using the information in Section 9.3 "Customer Service Department". NOTE In the event of an emergency, turn OFF the controller switch. This will cause all pumps to immediately stop.
Page 41: Section 2 Fluid System Connections And Accessories
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 2.1 Introduction This section discusses general fluid system connections, and the installation of fluid connection accessories, temperature/pressure control accessories, and optional kits and attachments. DANGER RISK OF INJURY. THIS EQUIPMENT PRODUCES HAZARDOUS PRESSURES.
Page 42: Ports
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories Pressure Transducer Pressure Nut and Transducer Ferrule Nut and Collar Plug Plug Port Tubing to Valve Tubing to Valve Figure 2-1 Liquid system plumbing connections (65x on left) 2.2.2 Installation Tips •...
Page 43: Tubing Requirements
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 2.2.3 Tubing The tubing must be cut squarely to prevent possible problems. Square Requirements ends are easier to insert through the ferrule and will decrease dead volume.
Page 44: Installing The 65X Fitting
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 2.2.4 Installing the 65x Fitting F250 Fitting and Refer to Figure 2-2 for components and fittings for the SyriXus 65x. Connection Tubing Gland Nut Valve or Fitting Body...
Page 45: Draining Overflow
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 2.2.5 Draining Overflow The drip tray outlet on the pump cylinder provides a means of draining fluid from seal leakage. Use a section of 1/4” ID flexible tubing to divert the leakage away from the pump.
Page 46: Drain Tube Installation And Wash Gland Connection
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories NOTE If the primary pump seal fails, the pressure of the delivery fluid will be exerted on the secondary (wash gland) seal. During operation, the unused wash gland outlet should always be uncapped and routed for either recirculation or drainage.
Page 47: Cylinder Washing - Nitrogen Purge
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 2.2.7 Cylinder Washing - All pumps that do not have a wash gland are equipped with a purge Nitrogen Purge connector tube near the top of the pump body. The purge connector enables the pump cylinder beneath the piston to be purged with nitrogen, reducing the presence of atmospheric oxygen below the piston.
Page 48: Fluid Connection Accessories
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories Using nitrogen, blow out any liquid that remains inside the cylinder, transducer port, and tubing. Using shop air pressure is not recom- mended due to the possibility of compressor oil being present in the air.
Page 49: Manual Outlet Valve Kit
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories Table 2-2 Manual Refill Kits Pump Model Part Number nut, ferrule 1000x 68-1247-117 500x 68-1247-083 260x 68-1247-077 system pre-bent 68-1247-127 SST tubing spacer block PTFE tubing stainless steel tubing valve filter...
Page 50: Outlet Valve Package Connection
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories NOTE When nuts are torqued to the cylinder cap ports, the pressure read- ing may be affected. If the pressure no longer reads zero, release the pressure in the cylinder and press ZERO PRESS. Refer to Sec- tion 3.8.11 "ZERO PRESS"...
Page 51: Temperature And Pressure Controls
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 2.4 Temperature and Some applications, such as those with very low flows, may require Pressure additional measures to maintain steady rates. Controls 2.4.1 Temperature The optional cylinder temperature control jacket assists in main- Control Jacket taining cylinder temperatures (-30 to 100 °C) by circulating liquids, such as water or water/ethylene glycol solution, through the 1/4”...
Page 52: Temp. Control Jacket Packages
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories Table 2-4 Temp. Control Jacket Packages Description Part Number Temperature Control Jacket Packages for: 68-1268-029 260x 68-1268-005 500x, 500xv, 1000x 68-1247-115 O-rings (2) for Temperature Control Jackets: AS 568A-225 EPDM 260x AS 568A-227 EPDM...
Page 53: Using The Wrench Package
Pumps that have been used for a while will be a little harder to unscrew the cylinder. A strap wrench or the wrench kit available from Teledyne ISCO can be used to assist with removing the cylinder. Instructions for using this wrench package will be pro- vided in Section 2.4.3 "Using the Wrench Package".
Page 54 SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 13. Select the proper size of cylinder holder clamps from the wrench package. 14. Position the cylinder holder clamps about 1/8” (3 mm) to 1/4” (6 mm) above the cylinder mount as shown in Figure 2-9. 15.
Page 55: Temperature Control Jacket Installation
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories 5. Position the jacket so that it touches the bottom of the cylinder cap. Caution Be careful not to damage the O-rings in the jacket when pushing them over the threads of the cylinder.
Page 56: Seal Options
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories Reassembling the Refer to Figure 2-10 for the following steps. Pump 1. Lower the cylinder/temperature control jacket assembly over the piston and push rod assembly. NOTE To avoid seal damage, ensure that the cylinder is aligned straight with the piston before lowering.
Page 57: Inside The Pump Cylinder: Wetted Materials
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories • Cylinder cap seal - The cylinder cap seal maintains the pressure within the cylinder. • Piston seal - The piston seal maintains the pressure within the cylinder.
Page 58: Seal Selection Chart
SyriXus Series Pumps Installation and Operation Guide Section 2 Fluid System Connections and Accessories The standard seals for each pump model satisfy the requirements of most applications, including those that use carbon dioxide, such as SFE. However, certain solvents and/or conditions require special cyl- inder seals to facilitate the application.
Page 59: Section 3 Basic Programming And Operation
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.1 Introduction This section will familiarize you with the SyriXus series pump con- troller and describe operating the pump under each of the various Modes: Constant flow, Constant Pressure, Dispense, and Refill. Pump setup and operation is regulated by the SyriXus series con- troller.
Page 60: Syrixus Series Controller To D Series Pump
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation Table 3-1 SyriXus Series Controller to D Series Pump D Series Pump Model Model Name Displayed 1000D 1000x 500D 500D 500HV 500D 260D 260D 260HP 260x 100DX 100DX 65DM 65DM...
Page 61: Rates, Units, And Limits
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.2.2 Rates, Units, and • To allow pump operation to be tailored to your application, both Limits the pressure and flow rate units may be set by the user. Refer to Section 3.4.1 "Flow and/or Pressure Units of Measure".
Page 62: Selecting Operating Parameters
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation Ending Time Current Pressure PS/MIN FINAL: 00:00:00 Final Pressure Rate • Line Four - Line four varies depending on the mode. The options presented on this line are softkey selectable, i.e. you use the softkeys (A - D) located below the display to choose the option.
Page 63: Flow And/Or Pressure Units Of Measure
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 1. UNITS 4. POWER FAILURE [STOP] 2. SELECT PUMP 5. SYSTEM RESET 3. REFILL 6. DISPLAY CONTRAST MORE RETURN Figure 3-1 Menu 1 program selections 3.4.1 Flow and/or Displayed units are user-selectable by selecting menu 1 item 1.
Page 64 SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation NOTE If more than one pump is connected to the controller, pump B, pump C, and pump D will be displayed on the fourth line, depending on how many pumps are attached.
Page 65: Power Failure [Stop]
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.4.4 Power Failure This feature allows you to set the activity of the pump in the event of a [STOP] power failure. Press ‘ 4’ to toggle this feature between [STOP] (to remain stopped after power is restored) or [CONT] (to automatically resume after power is restored).
Page 66: Pump Status
• Use softkey B, NEXT ID#, to scroll through the available ID numbers. These are 1-7. Six is the default, as this is the unit identifier used by the Teledyne ISCO LabVIEW™ software. For information on serial control, refer to Section 8 "Serial Interface".
Page 67: Types Of Valves
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation The number for the selected valve type will be blinking. Press ‘1’, ‘2’, or ‘3’ to select the correct valve type. Air Valve Electric Valve Ball Valve (500xv) Only Figure 3-6 Types of valves EAR99 Technology Subject to Restrictions Contained on the Cover Page...
Page 68: Menu Three
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.6 Menu Three Menu 3 provides programming options for poor fills, diagnostics, pressure calibration, alternative pressure inputs, pressure control setting, and pressure setpoint tolerance. To save all settings and return to the main menu, press PREVIOUS RETURN 1.
Page 69: Diff. Modes
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.6.4 Diff. Modes This feature allows the use of other analog inputs for pressure input to the pump. The standard pressure transducer MUST be connected to pump in order for this feature to work. •...
Page 70: Front Panel Keys
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.8 Front Panel Keys In addition to the menu options, certain functions and modes are selectable from the front panel keyboard. 3.8.1 CONST PRESS and These keys place the system in Constant Pressure Mode or Constant CONST FLOW Flow Mode, respectively.
Page 71: Limits Menu
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation To set the limits 1. Press LIMITS. To display the Limits menu, enter the number of the limit you wish to program. One of five limit setpoint menus will appear: MAX PRESS, MIN PRESS, MAX FLOW, MIN FLOW, or FLOW LIMIT.
Page 72 SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation Limits Programming In addition to setting the upper and lower limits, the pump also allows Options the user to specify whether they want: • The alarm message displayed •...
Page 73: Rapid Press
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.8.8 RAPID PRESS This option is available when operating in the Constant Flow Mode and in the two-pump concentration Gradient Mode. It allows rapid pressurization to a stable pressure point and then switches automati- cally to the Constant Flow setpoint.
Page 74: Zero Press
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.8.11 ZERO PRESS The ZERO PRESSURE key will correct pressure sensor drift. Before pressing ZERO PRESS, the pump should have port fittings installed, and be depressurized. 1. Open a valve, or loosen a fitting other than one on the pump, to ensure the cylinder pressure is actually zero.
Page 75: Independent Control Of Up To Four Separate Pumps
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.9.2 Independent A SyriXus series syringe pump controller can run up to four syringe Control of up to pumps independently of each other in either Constant Pressure, Con- Four Separate stant Flow Mode, Dispense Mode, or any combination of the three.
Page 76: Operating Modes
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.10 Operating Modes The pump has three Deliver Modes and one Refill Mode. NOTE When using a single controller to operate multiple pumps inde- pendently, you need to select the appropriate pump run screen before selecting a mode.
Page 77: Constant Flow
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.10.1 Constant Flow To set Constant Flow operation, use the following procedure: 1. Press CONST FLOW. “CFa” will be displayed in the upper left corner of the screen. This denotes that you will be defining con- stant flow parameters for pump A.
Page 78: Dispense Mode
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation NOTE If you make an error, press CLEAR ENTRY to delete one character at a time. 4. Press ENTER once the desired pressure is displayed. 5. Press RUN to initiate pump operation. 3.10.3 Dispense Mode For applications such as reactant feed and batch delivery where a specified volume is pumped, use Dispense Mode.
Page 79: External Control
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.11 External Control The pump can be externally controlled for pressure or flow rate oper- ation with an analog voltage or through the serial interface. The serial interface allows you to control the pump operation from a compatible personal computer or laptop that has an RS-232-C serial output.
Page 80: Preparation
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.12.2 Preparation Before programming the controller to accept the analog signal, you must know the maximum flow rate (Constant Flow) or the maximum pressure (Constant Pressure). NOTE The high and low limits you enter cannot exceed the pump specifica- tions: Normal input = 10V.
Page 81: External Control For Refill
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.12.3 External Control for The refill option allows you to set the refill rate, or set the pump to Refill automatically refill when a certain volume is reached. From Menu 2, the system can be programmed to automatically refill a pump based on an external analog signal, with a range of 0 to 11.5 volts.
Page 82: Setup
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation 3.12.5 Setup First, select the desired operating mode by pressing CONST PRESS CONST FLOW and selected EXTERNAL pump are displayed) (On/Off) MORE SET RANGE Enter Vmax value Figure 3-13 Button sequence for external control setup When the FULL SCALE INPUT RANGE entry field is flashing, pressing the STOP key once will exit the Entry Mode and discard the...
Page 83: Analog Flow Rate And Volume Output Options
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation • If two pumps are used with the controller, the second control wire should be connected to terminal 2, under DIGITAL INPUT. • If three pumps are used with the controller, the third control wire should be connected to terminal 3, under DIGITAL INPUT.
Page 84: Current Loop Output
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation A controller running one to two pumps can also output cylinder volume on the remaining outputs. The volume output range is not adjustable. Full scale is equal to one pump stroke. Figure 3-15 Output range selection jumpers Table 3-4 Analog Output Options (Connections to Female 25 Pin Sub-D) Pin No.
Page 85: Analog Output Signal/Sub-D Pins For 4-20Ma
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation Table 3-5 Analog Output Signal/Sub-D Pins for 4-20mA Pin # Channel Data Type/Pump Pump A Flow Rate Pump A Flow Rate (Signal Return) Pump A Pressure Pump A Pressure (Signal Return) Pump A Volume Remaining Pump A Volume Remaining (Signal Return) Pump B Flow Rate...
Page 86: Analog Output Signal/Sub-D Pins For 0-10 Vdc
SyriXus Series Pumps Installation and Operation Guide Section 3 Basic Programming and Operation Table 3-6 Analog Output Signal/Sub-D Pins for 0-10 VDC Pin # Channel Data Type/Pump Pump A Flow Rate Pump A Flow Rate (Signal Return) Pump A Pressure Pump A Pressure (Signal Return) Pump A Volume Remaining Pump A Volume Remaining (Signal Return)
Page 87: Section 4 Gradient Pumping For Pressure, Flow, And Concentration Modes
All Teledyne ISCO syringe pump models can be used in Gradient Mode, but SyriXus model 65x requires special hardware for this mode.
Page 88: Connecting The System
Detail" for standard port information. When making fluid connections that use ferrules, be sure to use the ferrules supplied for that pump by Teledyne ISCO. Push the tubing completely into the connector and finger-tighten. Then tighten with a wrench to clamp the ferrules onto the tubing.
Page 89: Inlet Connections
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes A gradient pumping system includes high-pressure, two-way valves that connect the pump inlets to fluid reservoirs, and the pump outlets to the gradient mixer (dual-pump system) or other apparatus (single-pump system).
Page 90: Outlet Connections
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes FROM REFILL KIT ASSEMBLY pre-bent SST tubing spacer block SST tubing 2-way valve APPARATUS Figure 4-2 Pump inlet connections 4.2.2 Outlet Connections Valve components and connections are shown in Figure 4-3. 1.
Page 91: Dual-Gradient System Connections
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes FROM REFILL KIT ASSEMBLY pre-bent SST tubing spacer block 5.1 cm length of 1/8” SST Tubing 2-way valve APPARATUS Figure 4-3 Pump outlet connections 4.2.3 Dual-Gradient Connection of the dual-gradient system requires two refill valve kits System...
Page 92: Dual-Gradient System Connections (Inlet Valves (A & C) Are From Refill Valve Kits)
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes NOTE Teledyne ISCO does not provide applications support for this pro- cess. OUTLET 1/8” OD X 0.069”ID INLET SST TUBING OUTLET (from INLET...
Page 93: Single-Pump Gradient Programming
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes 4.3 Single-Pump A single-pump gradient program is based on either pressure or flow, Gradient and controlled by either time duration (in minutes) or rate of change Programming (units per minute).
Page 94 SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes FILE# 1 STEP# 1 STORE TO EXIT 1. INIT = 0PSI 3. RATE = 0:00PSI/MIN 2. FINAL = 0PSI 4. DURATION = 1.0MIN INSERT DELETE FILE# 1...
Page 95: Dual-Pump Concentration Gradient Programming
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes 4.4 Dual-Pump Two-pump concentration gradients enable proportionate use of two Concentration different fluids that combine at the mixer (refer to Figure 4-4 and Gradient Figure 4-5).
Page 96 SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes 5. To proceed to the programming screen, press STEP FWD (B). The programming screen will appear, with the file number and step number at the top of the screen. FILE# 1 STEP# 1 STORE TO EXIT...
Page 97: Review, Revise, & Hold Options
SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes 4.5 Review, Revise, While in the programming menu, you can also: & Hold Options • Delete - To delete the current step, press DELETE (D). A deleted step cannot be recovered.
Page 98 SyriXus Series Pumps Installation and Operation Guide Section 4 Gradient Pumping for Pressure, Flow, and Concentration Modes GRADIENT ACTION=HOLD FINAL VALUE NEXT_ACTION PREVIOUS To scroll through the four options, press NEXT_ACTION (A). When you have reached the desired option, press PREVIOUS (D) to save and exit.
Page 99: Continuous Flow Air Valves
This flow irregularity can be measured as a pressure fluctuation. Teledyne ISCO pressure fluctuation at switchover is ~0.35 bar (at system backpressures from 6.9 bar to the single-pump maximum).
Page 100: Continuous Flow Air Valves Overview
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation Table 5-2 Continuous Flow Technical Specifications-Air Valves Pressure fluctuation at switchover 5 psi (0.35 bar), at system backpressures from 100 psi (6.9 bar) to the single-pump maximum.
Page 101 To install the air valve package, refer to the installation guide sup- plied with the valve assembly. A copy of this guide can also be down- loaded from the Teledyne ISCO website. Figure 5-1 represents the SyriXus 500x pump valve assembly; the other SyriXus series pump models will be similar.
Page 102 SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation One controller can operate up to two pairs of pumps and control the valves at the same time. The black wires of the valve assembly on the first pair of pumps need to be attached to the DIGITAL OUTPUT con- nectors labeled ‘A’...
Page 103: Controller Accessory Connector
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation SyriXus 500x Shown, Other Models are Similar (Front View) Model Number Label on Tubing System Outlet Pre-Swaged Connector Fitting (Typical) Check Valve Check Valve Actuator Actuator To Controller To Controller...
Page 104: Actuator Wire Labels
(Red) NOTE Teledyne ISCO has historically identified the pump to the left of the dual valve assembly as ‘A’ (‘C’) and the one to the right of the valve assembly as ‘B’ (‘D’) when facing the front of the equipment. The...
Page 105: Continuous Flow Electric Valves
. Electric valves use brush type electric motors that can be an ignition source. Using electric valves with flammable fluids is not rec- ommended or supported by Teledyne ISCO. The electric valve option is only available for the SyriXus 260x, 500x and 1000x. Electric valves require the pump controller to be equipped with a valve driver board.
Page 106: Continuous Flow Electric Valves Overview
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation Table 5-5 Continuous Flow Technical Specifications - Electric Valves Pressure fluctuation at switchover 5 psi (0.35 bar), at system backpressures from 100 psi (6.9 bar) to the single-pump maximum.
Page 107 SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation 5.3.2 Electric Valve An installation instruction sheet is provided with each valve package Installation with specific details related to the valve assembly. The valve package installation is very similar between the pump models, with slight dif- ferences in tubing connections.
Page 108 SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation One controller can operate only one valve assembly (either the single valve assembly or the dual valve assembly) when the electric valve option is used. DANGER THE ELECTRIC VALVE OPTION USES DC SERVO MOTORS THAT CAN GENERATE A SPARK WHEN...
Page 109: Electric Valve Installation
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation SyriXus 260x High-Temp Shown, Other Models are Similar (Front View) Overhead View Motor Pre-Swaged Fittings Outlet Valve Pump Tubing Solvent Inlet Valve Support Plate Model Identifier Controller Connector Valve Mount Screws/Washers Figure 5-3 Electric valve installation...
Page 110: Supplied Valves
• Check valves Do not use spring-loaded check valves if refilling at atmospheric pressure; the pump seals are not designed to draw against a vacuum. The balls in Teledyne ISCO check valves are closed by gravity. • Powered valves These are electrically triggered from the pump controller and could be air or electrically actuated.
Page 111: Continuous Flow Mode
You must operate the two pumps manually for initial setup (i.e. refill and purging of air). If the Teledyne ISCO air valve package is used, the air valves are switched through ACCESS CTRL. When air valves are used, lights on the air valve actuator indicate which valves are open.
Page 112: Keystrokes To Specify Valve Type
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation ACTIVE PASSIVE ELECTRIC MORE PREVIOUS Press 3x to return to run screen VALVE Figure 5-4 Keystrokes to specify valve type • Volume totalizer - The total volume delivered is displayed in liters at the top right corner of the screen.
Page 113: Constant Flow Mode
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation 5.5.2 Constant Flow Continuous Flow Mode is found under the multi-pump options on Mode Menu 2. Once you have accessed the multi-pump options, select Con- stant Flow Mode or Constant Pressure Mode.
Page 114 D series syringe pump controller (refer Section 3.2.1 "Controller Models"). Teledyne ISCO pumps are normally operated with a check valve in the refill line to prevent backflow into the working fluid source reservoir when the inlet valve is opened. This backflow results from the pres-...
Page 115: No Check Valve
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation NOTE A check valve is not usually used with Ball Valves because of the imposed flow resistance. It is recommended to turn ON the NCV feature before operating the valves.
Page 116: To Run Or Stop
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation 2. T – changes the time delay between the end of the OINT pump stroke and the opening of the inlet valve. The default is 5 seconds but can be set to any value between 1 and 99 seconds.
Page 117: Tips For Running Continuous Flow
SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation Always verify the valve settings before running a program. If a con- troller is reset or moved to a different power source, it will revert back to default settings (Active).
Page 118 SyriXus Series Pumps Installation and Operation Guide Section 5 Continuous Flow Introduction, Installation, and Operation • Temperature changes can cause pressure fluctuations. For available temperature control options, contact Teledyne ISCO. • For correct overpressure response, shutdown must be set to under options.
Page 119: Section 6 Modifier Addition
However, due to the higher operating pressure and the AE F250C fittings, the 65x requires hardware that is not listed in this section. Contact Teledyne ISCO customer service for details on configuring a 65x pump for this type of operation.
Page 120: Example Of Hardware
ISCO) to each of the pumps as shown. 2. Attach one manual outlet kit to each of the two pumps (also available from Teledyne ISCO). 3. Using metal tubing, attach a user supplied check valve to each of the outlet ports on the outlet valves as shown.
Page 121: Continuous Flow System
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition NOTE The pump with the primary working fluid needs to be connected to the PUMP A electrical connector on the controller, and the pump being used for the modifier needs to be connected to the PUMP B electrical connector on the controller.
Page 122 NOTE Continuous flow modifier addition is not recommended with the SyriXus 65x pump model. To use a 65x, either the air valve option would be employed, or a user provided electric valve arrangement would be required. Configurations for supercritical CO...
Page 123: Modifier Mode Setup
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition 6.1.4 Modifier Mode When first setting up a system for Modifier Addition Mode, the pumps Setup should be zeroed and filled. To accomplish this, the pumps should first be placed under independent control - Constant Pressure Mode. NOTE Once the modifier system is operating, the system does not need to be zeroed before subsequent refilling.
Page 124: Two-Pump Operation
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition To Fill Pump A with 1. Place the pumps in Independent Control - Constant Pressure Supercritical Fluid Mode, as detailed above. (Helium Head-Space 2. Be sure the pumps are stopped. Tank) 3.
Page 125: Programming
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition When pump B needs refilling, it must first be placed under inde- pendent control, as you must run it to return 5-10 mL of modifier back through the valve to be sure that the cylinder is free of air. NOTE To properly equilibrate the pumps, they both must be delivering fluid.
Page 126: Minimum Modifier Pressure Screen
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition I F Y O U R M O D I F I E R B O I L S A T R O O M T E M P E N T E R T H E M I N I M U M P R E S S T O P R E V E N T V A P O R 5 0 P S I...
Page 127: Refilling
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition NOTE Flow MUST be established through the system at or before the time “EQUILIBRATE” is displayed. To properly equilibrate the pumps, they must be delivering fluid. 14. Once the message R is displayed, modifier will be deliv- UNNING ered at the selected rate.
Page 128: Refill Pump B (The Modifier Pump)
SyriXus Series Pumps Installation and Operation Guide Section 6 Modifier Addition 6.3.2 Refill Pump B (the 1. Press STOP. Modifier Pump) 2. Close the outlet valves of both pumps. 3. Take the pumps out of Modifier Addition Mode by pressing soft- key ‘B’...
Page 129: Section 7 Modbus Configuration
The Modbus RTU protocol implementation uses a 2-wire RS-485 con- nection. The RS-485 connections are located on the DB25 connector labeled RS-232/RS-485. Teledyne ISCO recommends connecting the ground reference wire as shown in Figure 7-1. The matching con- nection is a D-Subminiature 25-pin standard plug, such as a TE Con- nectivity AMP connector (P/N 747912-2) from an electronic parts supplier.
Page 130: Modbus Tcp/Ip
SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration 7.1.2 Modbus TCP/IP The Modbus TCP/IP protocol implementation uses an Ethernet con- nection. The Ethernet connections are located on the optional DB25 connector labeled USB/Ethernet. Use optional cable (P/N 60-1244-488) and coupler (P/N 149-9052-00) to connect to your Ethernet.
Page 131: Modbus Rtu Configuration Options
SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-3 Modbus RTU Configuration Options Item Value Description Baud Rate 300, 1200, 2400, 4800, 9600, 19200, Communication Speed (Default 19200) 38400, 57600, 115200 Slave ID 1-247 Device address (Default 1) Parity Even 1 Stop Bit, Odd 1 Stop Bit, None 1 Communication parameters (Default Even 1 Stop Bit)
Page 132 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-6 Coils Register Address Type Size Description 00001 1 BIT 0: Stop Pump A 1: Run Pump A 00002 1 BIT 0: Stop Pump B 1: Run Pump B 00003 1 BIT 0: Stop Pump C 1: Run Pump C...
Page 133 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-6 Coils (Continued) Register Address Type Size Description 00032 1 BIT 1: Activates the automatic rapid pressurization cycle (Constant Flow Mode only) for Pump A 00033 1 BIT 1: Activates the automatic rapid pressurization cycle (Constant Flow Mode only) for Pump B 00034 1 BIT 1: Activates the automatic rapid pressurization cycle (Constant Flow Mode only)
Page 134 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-6 Coils (Continued) Register Address Type Size Description 00065 1 BIT 0: AUTO REFILL C OFF 1: AUTO REFILL C ON 00066 1 BIT 0: AUTO REFILL D OFF 1: AUTO REFILL D ON 00067 1 BIT 0: AUTO FILL A OFF...
Page 135 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-6 Coils (Continued) Register Address Type Size Description 00097 1 BIT 0: Underpressure Display OFF 1: Underpressure Display ON 00098 1 BIT 0: Underpressure Shutdown OFF 1: Underpressure Shutdown ON 00099 1 BIT 0: Overflow Alarm OFF 1: Overflow Alarm ON...
Page 136: Holding Registers
SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-6 Coils (Continued) Register Address Type Size Description 00133 1 BIT 1: Motor failure Pump D 00134 1 BIT 1: POOR FILL A ERROR 00135 1 BIT 1: POOR FILL B ERROR 00136 1 BIT 1: POOR FILL C ERROR 00137...
Page 137 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-7 Holding Registers (Continued) Read/ Register Address Type # Registers Description Units Write 40023 FLOAT Flow rate setpoint for mL/min; mL/hr; µL/min; µL/hr multi-pump pair CD in Continu- ous Flow Constant Flow Mode.
Page 138 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-7 Holding Registers (Continued) Read/ Register Address Type # Registers Description Units Write 40069 FLOAT Maximum flow limit in Constant mL/min; mL/hr; µL/min; µL/hr Pressure Mode for Pump C. 40071 FLOAT Maximum flow limit in Constant...
Page 139 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-7 Holding Registers (Continued) Read/ Register Address Type # Registers Description Units Write 40117 FLOAT Volume at which Pump A will milliliters automatically switch to Refill Mode if AUTO REFILL A is ON. 40119 FLOAT Volume at which Pump B will...
Page 140 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-7 Holding Registers (Continued) Read/ Register Address Type # Registers Description Units Write 40147 FLOAT POOR FILL MARK D % VALID ENTRYS:10,20,30,40,50,60, 70,80,90 40149 FLOAT Volume being dispensed by milliliters Pump A in Dispense Mode.
Page 141 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Table 7-7 Holding Registers (Continued) Read/ Register Address Type # Registers Description Units Write 40229 FLOAT Absolute maximum refill rate for mL/min; mL/hr; µL/min; µL/hr Pump D. 40231 FLOAT Maximum volume for Pump D.
Page 142 SyriXus Series Pumps Installation and Operation Guide Section 7 Modbus Configuration Intentionally Left Blank EAR99 Technology Subject to Restrictions Contained on the Cover Page 7-14...
Page 143: Section 8 Serial Interface
This function is supported by the Teledyne ISCO LabVIEW™ toolkit. You can write your own custom program to control Teledyne ISCO syringe pumps using any suitable programming language, such as BASIC or C++. This section provides the syntax and responses for serial commands.
Page 144: External Control Connector Serial Pin Connections
300, 1200, 2400, 4800, 9600, 19200, 38400, 57600, and 115200 are supported by the SyriXus series pumps, but are not part of the Teledyne ISCO defined communications standard. Electrical standards are RS-232-C; connector pin usage is outlined in Table 8-1 "External Control Connector Serial Pin Connections".
Page 145: Usb Interface
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface 8.3 USB Interface The Teledyne ISCO SyriXus series syringe pump controller may include an optional USB interface. The USB connections are located on the pump controller rear panel labeled USB/Ethernet. The con- nector pin usage is outlined in Table 8-2 "USB Interface Pin Connec-...
Page 146: One Controller
Figure 8-1. This cable may also be available from a computer parts store. If your computer does not offer a serial port, a USB to serial adapter may work, but Teledyne ISCO does not support or aid in the use or connection of these devices.
Page 147: Three Or More Controllers
RS-232-C limitations of speed and cable lengths. Although the functions still exist in the controller to allow the serial control of multiple controllers, Teledyne ISCO no longer offers the interconnection cables for three or more controllers and has discon- tinued the support of this function.
Page 148: Serial Control Check List
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface 8.5 Serial Control In order to build and operate a custom program for Teledyne ISCO Check List syringe pumps, you must have the following: 1. Sample program 2. LabView Compiler (optional - purchased separately) 3.
Page 149: Verify Operation
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface If several units are being configured, Teledyne ISCO suggests placing a label on the rear of each instrument listing the ID number and baud rate. This will help identify the controller in the future and prevent assigning the same ID number to two controllers or assigning an incorrect baud rate.
Page 150: Universal Driver From Teledyne Isco
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface 8.7.2 Universal Driver The Universal Driver from Teledyne ISCO is a serial software driver from Teledyne that can translate commands for a variety of compilers. When you are ISCO...
Page 151 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of BASIC Program to Demonstrate Conversion of Pump Commands to DASNET Frames 1 CLS : Z$ = "": PRINT "INPUT ALL ENTRIES IN CAPITAL LETTERS" 5 INPUT "INPUT UNIT ID >> ", UNITNUM’GET UNIT NUMBER 30 REM OPEN COM PORT SET FOR COM2 EDIT TO COM1 IF NEED 40 OPEN "COM2:1200,N,8,1,ASC"...
Page 152 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of C Program Demonstrating Conversion of Pump Commands to DASNET Frames #include<stdio.h> #include<conio.h> #include "b:comm.c" /* edit to path needed */ /* a ’C’ example of DASNET serial control */ int conv_das();...
Page 153 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of Visual C++ Program Showing Conversion of Pump Commands to DASNET Frames // visual C++ version 10.0 // Dasnet.cpp : Defines the entry point for the console application. // A ’C, C++’...
Page 154 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of Visual C++ Program Showing Conversion of Pump Commands to DASNET Frames (Continued) CloseHandle(hCom); printf("Comm intr released\n"); void conv_das(char *in, char *out) unsigned sum; char *c_ptr; c_ptr=out; *out++=unitnum+0x30; /* put id first */ *out++='R';...
Page 155 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of Visual C++ Program Showing Conversion of Pump Commands to DASNET Frames (Continued) int count=0; n=strlen(out); // start of packet if(!WriteFile(hCom, "\r", 1, &dwBytesRead, NULL)) //error occurred. Report to user. // data if(!WriteFile(hCom, out, n, &dwBytesRead, NULL)) //error occurred.
Page 156 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of Visual C++ Program Showing Conversion of Pump Commands to DASNET Frames (Continued) Sleep(2000); exit(1); switch(speed) case 19200: dcbSerialParams.BaudRate=CBR_19200; break; case 38400: dcbSerialParams.BaudRate=CBR_38400; break; case 57600: dcbSerialParams.BaudRate=CBR_57600; break; case 115200: dcbSerialParams.BaudRate=CBR_115200;...
Page 157 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Example of Visual C++ Program Showing Conversion of Pump Commands to DASNET Frames (Continued) char buf[2]; buf[0]=0; /* get char if there if(!ReadFile(hCom, buf, 1, &dwBytesRead, NULL)) //error occurred. Report to user. return buf[0];...
Page 158 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface • Destination - The 1-digit unit identification number of the instrument to receive the message. • Acknowledgment - One character to indicate the success of the previous transmission. There are three possibilities: (1) E means error, resend the message immediately (E is sent by the network controller only.
Page 159 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Decimal Format Step 1 559 = 82 + 51 + 48 + 52 + 83 + 84 + 79 + 80 Using MODULO Step 2 2R47  47 = 559 · ·...
Page 160 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface NOTE A [CR] must start the network. The controller is checking for the presence of unit #1 but will get no response in 200 ms because there is no unit 1. Network Controller and Master Unit #0 1R 5D[CR] Still no response.
Page 161 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface The same sequence would occur with all messages. Since the master and the network controller are combined in this example, the relaying of messages is not necessary. Unit 6 R027SERIES=1240-02__, Model ___D PUMP, REV __XX[CR] The pump responds with identity and software revision letter.
Page 162: Serial Commands For The Syrixus Series Pumps
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface It is important to follow this format. Spaces are ignored anywhere within the message field. Commands must be in uppercase letters. The network definition allows multiple commands in a message field when delimited by semicolons, but the SyriXus series controller is l i m i t e d t o s i n g l e c o m m a n d s .
Page 163 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Table 8-3 Serial Commands (Continued) Command (Refer to Description NOTE 1 & NOTE 2 DIGITAL = xxxxxxxx Sets the digital output either High or Low, where ‘x’ is either ‘H’ or ‘L’. The order cor- responds with the outputs 1-8.
Page 164 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Table 8-3 Serial Commands (Continued) Command (Refer to Description NOTE 1 & NOTE 2 IDENTIFY Pump responds ‘SERIES=1240-0___, MODEL ____D PUMP; REV__.’ For each pump, REV__ is the internal pump program software revision. (For example, if the controller was attached to two 100DMs, the message would read ‘SERIES=1240-024, MODEL 100DM PUMP;...
Page 165 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Table 8-3 Serial Commands (Continued) Command (Refer to Description NOTE 1 & NOTE 2 LIMITS Returns the pressure and flow rate limits. LIMITSB LIMITSC LIMITSD LOCAL Returns the instrument to local control. Front panel control is enabled and all motors are stopped (if control was previously remote).
Page 166 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Table 8-3 Serial Commands (Continued) Command (Refer to Description NOTE 1 & NOTE 2 MODE MODE A P, B P, C P, D P A, B, C, D refer to the pump connection. P- Constant Pressure F- Constant Flow R- Refill...
Page 167 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Table 8-3 Serial Commands (Continued) Command (Refer to Description NOTE 1 & NOTE 2 REFILL=# Enter # to designate refill rate. REFILLB=# REFILLC=# REFILLD=# REMOTE Disables controller front panel control and enables all serial commands. Stops all motors (if control was previously local).
Page 168: Get Status Command
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Table 8-3 Serial Commands (Continued) Command (Refer to Description NOTE 1 & NOTE 2 ZEROA ‘Zeros’ the pressure sensor offset. ZEROB ZEROC ZEROD ZERODIFF1 ‘Zeros’ the pressure sensor offset for the respective analog input. ZERODIFF2 ZERODIFF3 NOTE 1...
Page 169: Get Status String 'Gg' Command
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface R049=37510,-00128, 01017, 06981, 05743, 00421, 00442, 00449, 25046,11111000,CS Pressure Pump A Pressure Pump B Digital Inputs 1—8 Pressure Pump C 1 = High Units = (psi*5) 0 = Low Resolution = 0.2 psi ALOG1 —...
Page 170: Get All Status String 'G&' Command
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Figure 8-5 Get all status string ‘G&’ command EAR99 Technology Subject to Restrictions Contained on the Cover Page 8-28...
Page 171: Get All Status Strings From Four Pump Operation 'G&2' Command
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Figure 8-6 Get all status strings from four pump operation ‘G&2’ command EAR99 Technology Subject to Restrictions Contained on the Cover Page 8-29...
Page 172: Gradient Download Command
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface 8.8.2 Gradient Download This command downloads a step from the SyriXus series pump to the Command PC. This command will respond with ‘PROBLEM=INVALID COMMAND’ if the file or step does not exist. If the file and step is valid, the controller will respond as shown in Figure 8-7 and Figure 8-8.
Page 173: Gradient Download Commands - Two-Pump Flow Gradient
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Step Number 01 LGUL,F:xx,S:01,FL:xxx.xxxx,IB%:xx.xx,FB%:xx.xx,D:xxxx.x, Gradient Duration Range = (0000.1 – 9999.0 minutes) Final %B Range = (00.00 – 100.0) Initial %B Range = (00.00 – 100.0) Gradient Total Flow Rate Units = milliliters/minute Range = (000.0000 –...
Page 174: Gradient Upload Commands - Single-Pump Flow Gradient
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Step Number 01: LGUL,F:xx,S:01,FL:xxx.xxxx,I %:xx.xx,F %:xx.xx,D:xxxx.x, Gradient Duration Range = (0000.1 – 9999.0 minutes) Final Gradient Percentage Range = (00.00 – 100.0) Initial Gradient Percentage Range = (00.00 – 100.0) Gradient Total Flow Rate Units = milliliters/minute Range = (000.0000 –...
Page 175: Gradient Upload Commands - Two-Pump Flow Gradient
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Step Number 01 LGUL,F:xx,S:01,FL:xxx.xxxx,IB%:xx.xx,FB%:xx.xx,D:xxxx.x, Gradient Duration Range = (0000.1 – 9999.0 minutes) Final %B Range = (00.00 – 100.0) Initial %B Range = (00.00 – 100.0) Gradient Total Flow Rate Units = milliliters/minute Range = (000.0000 –...
Page 176: Range Command
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface 8.8.4 Range Command The RANGE command provides scaling information for the system parameters. For example, 6R006RANGEA34 may return a string similar to the one shown in Figure 8-12. R02CP= 10000,F=2500000,R=30.00000,V=102.9600,40 Checksum Max Volume = 102.96 Units = milliliters...
Page 177: Error Messages
SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface 8.8.5 Error Messages If an error occurs in a message, one of the following responses will be sent. The format of an error message is ‘PROBLEM=_____.’ Refer to Table 8-4 "Error Messages". Table 8-4 Error Messages Error Description...
Page 178 SyriXus Series Pumps Installation and Operation Guide Section 8 Serial Interface Intentionally Left Blank EAR99 Technology Subject to Restrictions Contained on the Cover Page 8-36...
Page 179: Section 9 Pump Maintenance, Troubleshooting, And Servicing
Other components will require calling our customer service department to assist in selecting the right parts and guidance on installation. NOTE For replacement part numbers and parts ordering, use the Teledyne ISCO located at https://store.teledyneisco.com/. Online Store RISK OF ELECTRIC SHOCK - DISCONNECT THE ELEC- TRIC POWER BEFORE SERVICING.
Page 180: Introduction
To view the schematic drawings referred to in this section, first find the serial number for your unit. Contact the Teledyne ISCO Customer Service Department and request a copy of the schematic for your unit.
Page 181: Customer Service Department
• Do not return the pump without first providing written guarantee that it has been decontami- nated of hazardous or potentially lethal materials. Teledyne ISCO reserves the right to refuse shipment if no decontamination assurance has been pro- vided prior to shipment. Failure to decontaminate a pump may result in legal action taken by state or federal authorities.
Page 182: Resetting The System
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9.5 Resetting the This action completely deletes user programmed settings, erasing System all programs and returning the controller to factory default settings. Record your program settings and parameters before performing a reset operation.
Page 183: Analog Inputs
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9.6.2 ANALOG INPUTS Displays the values of all analog inputs in volts. In the left column, with all connected pumps at zero pressure, the pressure readings should be near zero.
Page 184 SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Flag Sensor Figure 9-3 Optical sensor, interrupted by flag (bottom sensor shown, rear tower cover plate removed) Select the pump to be tested (A, B, or C). TRAVEL LIMIT SENSE AND MOTOR POSITION Upper limit = 0 Lower limit = 0 Tach counter = ###########...
Page 185: Serial Test
If the unit passes the internal port test, next perform the I/O test. Use a null-modem cable (available from Teledyne ISCO, P/N 480-7996-00 or most computer parts stores) to connect the 9-pin serial output port of a computer to the 25-pin serial RS-232-C port, as shown in Figure 9-4.
Page 186: Removing The Case Top
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Run the test again. The screen should display: Rec: [whatever is typed from PC] Sent: ****** Serial Test #### ***** Press any key to exit Anything typed on the computer keyboard should appear in the top line on the controller display.
Page 187: Pump Case Top Removal
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9.7.2 Pump Case Top Some maintenance and troubleshooting procedures require accessing Removal the pump module interior. Remove the six screws holding the case top in place (three screws on each side). Lift the cover straight up and off. Figure 9-6 Pump case top screws (3 of 6 shown) - TBD 9.8 Test Points - TBD The following sections contain tables listing the most commonly used...
Page 188: Controller
9-7, configuration 1 or 2, depending on your pump motor type. For ® your convenience, a lubrication kit (containing Never-seez ® DUOLEC 1608 lubricants) is available from the Teledyne ISCO web store (P/N 601244271). Ball Screw Thrust Bearing (worm gear...
Page 189: Ball Nut
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Worm/Worm Gear The worm and worm gear are lubricated by a lubrication wheel. Apply DUOLEC 1608 directly to the felt part of the wheel until it is satu- rated.
Page 190: Cylinder Lock Screw
Figure 9-8 Cylinder lock screw 4. Unscrew the cylinder using either a strap wrench or the clamp and wrench in the Teledyne ISCO wrench package (P/N 60-1247-101) to not mar the cylinder’s outer surface. 5. Once the cylinder has been unscrewed, lift it straight up and off the piston and the push tube.
Page 191: Piston Seal (Excludes 30D)
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9.10.1 Piston Seal Sometimes dirt or other solids on the seal can cause leakage. (Excludes 30D) Removing and cleaning the seal may stop the leak and a new seal may not have to be used.
Page 192: All Other Piston Seals
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Model 1000x - Contact Teledyne ISCO Technical Service for additional instructions with these seals Figure 9-9 Break-in procedure for aqueous seals 9.10.3 All Other Piston 1. Access the piston seal as previously described.
Page 193: Wear Ring Cleaning And Replacement
Although the wear ring does not routinely need to be replaced, occa- Cleaning and sionally it becomes worn or damaged, depending on how the pump has been used. Teledyne ISCO recommends that when replacing the seal Replacement or cleaning the piston, check the wear ring for any signs of deterio- ration.
Page 194: Flushing The Cylinder
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9.12 Flushing the After cylinder/seal maintenance or during modifier (liquid solvent) Cylinder change, the pump cylinder should be flushed to remove possible residue. The way in which the cylinder is flushed will depend on your pumping system and whether you are pumping a liquefied gas such as CO (refer to Section 9.12.1 "Gas Solvent Changeover") or a liquid modifier such as methanol (refer to Section 9.12.2 "Liquid Solvent Changeover...
Page 195: Overpressure
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing To Clean the 1. Press . You will be asked to designate which > CONST FLOW Cylinder pump if more than one is present. 2. Press a softkey to run the desired pump. 3.
Page 196: Shear Key
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing WARNING The shear key is NOT a safety feature! It provides a measure of protection against damage to the equipment. Avoid overpressure situations by properly installing and programming the pumping system.
Page 197: Replacement Using Installation Tool
Shear Key Locking Pin Figure 9-11 Shear key replacement 9.13.2 Replacement Using A shear key installation tool is part of the Teledyne ISCO Wrench Installation Tool Package (P/N 60-1247-101). NOTE It may be easier to use a flat blade screwdriver in the shear key gap to pull the centermost half out first.
Page 198: Replacement
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing NOTE Use care not to push the pieces into the gear, making them more difficult to remove. 4. Insert the new shear key into the slot with the gap facing away from the shaft, as shown in Figure 9-11.
Page 199: Motor Brushes
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 4. Loosen the nut until the nut can be turned by hand. Tighten the nut to finger tight. 5. Torque the castle nut to 100 in-lbs (11.3 N-m). Do not repeat this torque.
Page 200: Motor Brush Disassembly
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Quick Disconnect/Motor Brush Retainer (One of Two) Figure 9-13 SyriXus series motor brush replacement Motor Brush Figure 9-14 Location of motor brushes A new brush is 1.1 cm long. Brushes should be replaced before they wear to less than 0.4 cm long.
Page 201: Inserting New Motor Brush
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 1. Unscrew the brush retainer from the motor using a straight edge screwdriver. 2. Pull the brush out of the slot and measure its length. If it is near- ing 0.4 cm, replace both brushes.
Page 202: Calibrations
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9.15 Calibrations It should not be necessary to readjust the instrument unless repairs have been made on the electronic circuitry or the controller cable length has been changed. 9.15.1 Pressure Transducer Calibration...
Page 203 SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 5. The limit sensor assembly includes two limit sensors, the wire harness and connector, and a conduit which protects the wires running between the sensors as shown in Figure 9-17. Depending upon when your pump was manufactured, the protective conduit may be directly fastened to the pump or retained with mounting tabs and cable ties.
Page 204: Limit Sensor Replacement
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Figure 9-17 Limit sensor replacement Figure 9-18 Installing the plastic mounts EAR99 Technology Subject to Restrictions Contained on the Cover Page 9-26...
Page 205: Limit Sensor Calibration
8. Ensure that the cylinder is unscrewed several turns. If you need to use a wrench, we recommend a strap wrench or wrenches in the Teledyne ISCO wrench package (P/N 60-1247-101) which will not mar the cylinder’s outer surface. 9. Center the pump bottom limit sensor with respect to the mount- ing screws and tighten them.
Page 206: Electric Valve Motor
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 9. Press STOP when the volume counter in the upper right-hand corner of the LCD reads 005.00 mL or less. 10. Press ‘A’ (FLOW RATE) and enter 5 mL/min. 11.
Page 207: Electric Valve Motor Adjustment
SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing 2. Inside the controller, attach the leads of a voltmeter across resis- tor R204 on the electric valve interface board. 3. Press ) > ACC CTRL PRGM GRAD 4.
Page 208 SyriXus Series Pumps Installation and Operation Guide Section 9 Pump Maintenance, Troubleshooting, and Servicing Intentionally Left Blank EAR99 Technology Subject to Restrictions Contained on the Cover Page 9-30...
Page 209: Calibration
9-11 Electrical connections, 1-15 shear key, 9-18 analog control, 3-21 solvent changeover, 9-16 Error messages - serial command, 8-35 Teledyne Isco Service, 9-2 External control, 3-21 torque limiter, 9-17 analog, 3-8 wear ring, 9-15 DASNET, 8-7...
Page 210: Technical Specifications
SyriXus Series Pumps Installation and Operation Guide Index power failure, 3-7 Specifications, 1-2–1-6 pump status, 3-8 Supercritical fluid extraction, 6-1 refill, 3-5 modifier addition, 6-7 serial options, 3-7 preparation for modifier, 6-5 total volume reset, 3-8 refilling the pumps, 6-9 Modes of operation, 3-18 System preliminary checkout, 1-18 Monitoring flow rate, 3-25...
Page 211 Compliance Statements Radio Interference Statement - FCC This equipment has been tested and found to comply with the limits for a class A digital device, pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.
Page 212 2-2 SyriXus 65x Fitting ........
Page 213: Optical Sensor, Interrupted By Flag
8-1 Serial network connection example - Single connection ....8-4 8-2 Serial network connection example - dual connection ..... . 8-5 8-3 Get status string ‘G’...
Page 214 1-1 65x Technical Specifications ......... . 1-2 1-2 260x Technical Specifications .

This manual is also suitable for:

Syrixus 260x Syrixus 500x Syrixus 500xv Syrixus 1000x

Teledyne SyriXus 65x Installation And Operation Manual

Quick Links

Related Manuals for Teledyne SyriXus 65x

Summary of Contents for Teledyne SyriXus 65x