Set Of The Dic Prism Slider - Nikon Multizoom AZ100 Instruction Manual

Set of the DIC Prism Slider

For the DIC prism slider installation, the zoom body is provided with a slot for it.
To perform the differential interference contrast (DIC) microscopy, remove the cover of the slot and attach a
slider suitable to the microscopy method and the illumination method. The slider must be selected depending
on the illumination method, the episcopic illumination or the diascopic illumination.
To perform the DIC microscopy, an illuminator for the episcopic illumination or the diascopic illumination is
required. For the procedure of the DIC microscopy, see the instruction manual for the AZ-ICI Coaxial
Episcopic Illuminator or the instruction manual for the AZ-STD Diascopic Stand and the AZ-STGD DIA
Stage.
Attaching the DIC prism slider
For the episcopic illumination:
Attach the AZ-PH EPI DIC Prism Holder to the slot on the
AZ100 and insert a DIC prism slider for the episcopic
illumination into the slot on the prism holder. Select a DIC prism
slider suitable to the objective in use. (Refer to the following
table.)
For the diascopic illumination:
Two DIC prisms are required. The first prism is inserted to the
position above the objective. Attach the AZ-AN DIA DIC Prism
Holder with Analyzer to the slot on the AZ100 and insert a DIC
prism slider for the diascopic illumination into the slot on the
prism holder. Select a DIC prism slider suitable to the objective
in use. (Refer to the following table.)
The second prism is inserted to the position at the illumination
side below the specimen. Select a DIC prism according to the
magnification of the objective and insert it into the DIC slot on
the AZ-STD Diascopic Stand. For details, see the instruction
manual for "AZ-STD Diascopic Stand the AZ-STGD DIA Stage."
Selection of a DIC prism slider
Each DIC prism slider has the same color code symbol of the corresponding objective. Check the color code
symbol on the objective to be used and use the DIC prism slider corresponding to the color code.
Ojective
1x
4x
5x
Operation of the DIC prism slider
Loosen the clamp screw on the side of the prism holder or the analyzer (for the diascopic illumination). To
place the DIC prism into the optical path, push in the DIC prism slider as far as it goes. To remove the DIC
prism from the optical path, pull the slider fully. Note that if the clamp screw is loosened too much the DIC
prism slider will be detached.
For the interference color adjustment, place the DIC prism into the optical path and rotate the prism position
knob at the edge of the slider to move the prism.
Chapter 3 Operation Procedures
DIC Prism Silder
Color Code
(for the episcopic illumination)
Gold
Red
Orange
* The DIC microscopy cannot be performed with the Plan Apo 0.5x or Plan Fluor 2x.
Prism holder or
analyzer (for the
diascopic
illumination)
(for the diascopic illumination)
AZ-EPS1
AZ-EPS1
AZ-EPS5
15
DIC prism slider
Set of the DIC Prism slider
DIC Prism Silder
AZ-DPS1
AZ-DPS1
AZ-DPS5