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Summary of Contents for Nikon ECLIPSE E200 MV Series
- Page 1 M588E 11.10.NC.1 (1/2) Microscope ECLIPSE E200 MV Series E200LED MV Series Instructions...
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Page 3: Introduction
Introduction Thank you for purchasing a Nikon product. This instruction manual is written for users of the Nikon ECLIPSE E200 MV Series and E200LED MV Series Microscopes. To ensure correct usage, read this manual carefully before operating the product. • No part of this manual may be reproduced or transmitted in any form without prior written permission from Nikon. -
Page 4: Table Of Contents
Contents Contents Introduction ....................i Safety Precautions ..................iv WARNING and CAUTION Symbols Used in This Manual .......iv Symbols on the Product................v WARNING ..................vi CAUTION ..................viii Notes on Handling the Product ..............ix Chapter 1 Nomenclature of Each Part............2 Chapter 2 Switches and Controls .............. - Page 5 Contents Chapter 5 Miscellaneous Operations............22 Oil-Immersion Observation ..............22 Torque Adjustment of the Coarse Focus Knob ........24 Upper Limit Bolt................24 Eye Level Raiser................25 Photomicrography and TV Microscopy ..........25 ND Filter for the Objective ...............26 Cord Hangers ................27 Chapter 6 Assembly................28 Assembly of Standard Set ...............28 Assembly of Additional Components..........30 Replacement of Consumable Materials..........32...
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Page 6: Safety Precautions
Safety Precautions To ensure correct and safe operation, read this manual before using the product. WARNING and CAUTION Symbols Used in This Manual Although this product is designed and manufactured to be completely safe during use, incorrect usage or failure to follow the safety instructions provided may cause personal injury or property damage. To ensure correct usage, read this manual carefully before using the product. -
Page 7: Symbols On The Product
Safety Precautions Symbols on the Product The symbols on the product indicate the need for caution at all times during use. Before operating a part labeled with the following symbols, refer to the instruction manual and read the relevant instructions. Symbol Meaning Biohazard... -
Page 8: Warning
Disassembling this product may result in electric shock or malfunction. Malfunctions and damage due to such mishandlings will not be warranted. Do not disassemble any part that is not indicated in this manual. If you experience problems with the product, contact your nearest Nikon representative. - Page 9 Notes on handling flammable solvents The following flammable solvents are used with the product: • Immersion oil (Nikon Immersion Oil for oil immersion objectives) • Absolute alcohol (ethyl alcohol or methyl alcohol for cleaning optical parts) •...
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Page 10: Caution
Intrusion of foreign matters into the product may also result in malfunctions. If liquids or foreign matters enter the product, do not use the product, and contact your nearest Nikon representative. Cautions on moving the product •... -
Page 11: Notes On Handling The Product
Notes on Handling the Product Handle with care. The product is a precision optical instrument. Handle the product with care and avoid physical shocks and vibrations. In particular, the precision of objectives may be lost by even weak physical shocks. Weak electromagnetic waves This product emits weak electromagnetic waves. - Page 12 Notes on Handling the Product Refocusing When changing specimens using the refocusing mechanism, gently lower the stage by hand taking care not to hit the field lens with the condenser holder (P.19). Make sure not to hit the field lens. Focus knobs Do not turn the right and left focus knobs simultaneously in opposite directions.
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Page 14: Chapter 1 Nomenclature Of Each Part
Nomenclature of Each Part The microscope is made up of the following components. (1) Main Body (2) Eyepieces Screwed on to the eyepiece tube. (3) Eyepiece Tube This is a binocular eyepiece tube. A trinocular eyepiece tube is available for photomicrography and TV microscopy. - Page 15 Chapter 1 Nomenclature of Each Part (2) Eyepieces (3) Eyepiece Tube (1) Main Body (4) Objectives (5) Condenser (8) Power Cord (6) Field Lens Unit (7) Lamp * The main body in this figure is an example of ECLIPSE E200 MV RS. * The light source for E200 MV series is halogen lamp, and the light source for E200LED MV series is white LED.
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Page 16: Chapter 2 Switches And Controls
Switches and Controls (1) Diopter ring (14) Power switch Adjust the diopter ring to When pressed to the “l” position, compensate for the difference power is turned on and the lamp between your right and left lights. When pressed to the “○” position, eyesight. - Page 17 Chapter 2 Switches and Controls (1) Diopter ring (2) Revolving (3) Stage nosepiece (11) Field diaphragm ring (4) Specimen holder (12) Field diaphragm centering screws (5) Condenser aperture diaphragm lever (14) Power switch (15) Brightness control dial (7) Condenser auxiliary lens (9) Longitudinal stage motion (Y Axis) knob (8) Filter holder...
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Page 18: Chapter 3 Microscopy Procedure (Quick Reference)
Microscopy Procedure (Quick Reference) Turn on the power switch. Press the power switch to the “l” position. Lower the condenser slightly down from its upper limit. Rotate the condenser focus knob to lower the condenser slightly down from its upper limit. Fully open the field aperture and condenser aperture diaphragms. - Page 19 Chapter 3 Microscopy Procedure (Quick Reference) Adjust the field diaphragm. (This adjustment is only for microscopes with the field diaphragm.) Turn the field diaphragm ring until the diaphragm image just circumscribes the viewfield. Perform the microscopy. ⇒P18 Whenever the objective is changed, adjust the condenser aperture and field aperture diaphragms.
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Page 20: Chapter 4 Microscopy Procedure (Detailed)
Microscopy Procedure (Detailed) ★ Note that the figures in this manual show the “stage motion knobs” located on the right side and the “condenser focus knob” located on the left side of the microscope body. These operating parts are located on the opposite side depending on the model of the microscope. The “coarse focus knob” and “coarse focus knob torque adjustment ring”... -
Page 21: Fully Open The Field Aperture And Condenser Aperture Diaphragms
Chapter 4 Microscopy Procedure (Detailed) Fully open the field aperture and condenser aperture diaphragms. (Field diaphragm control is only for microscopes with the field diaphragm.) (1) Turn the field diaphragm ring to the left limit to fully open the diaphragm. Field diaphragm ring (2) Turn the condenser aperture diaphragm lever to the left limit to fully open the... -
Page 22: Place The Specimen Slide On The Stage And Bring The Specimen Into The Optical Path
Chapter 4 Microscopy Procedure (Detailed) Place the specimen slide on the stage and bring the specimen into the optical path. (1) Place specimen slide on the stage with (1)(2) the coverglass facing upward. (2) Open the claw of the specimen holder with your finger at the root or the tip tilt and fix the specimen slide with the claw. - Page 23 Chapter 4 Microscopy Procedure (Detailed) (2) Look into the eyepiece with your right or left eye, and adjust the brightness of the viewfield by turning the brightness control dial. Turning the dial clockwise increases the brightness; turning the dial counterclockwise decreases it.) Darkens Brightens Brightness control dial...
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Page 24: Adjust The Diopter Ring On The Eyepieces
Chapter 4 Microscopy Procedure (Detailed) Adjust the diopter ring on the eyepieces. This procedure is to adjust the diopter ring on the eyepieces according to the difference between your right and left eyesight. This adjustment enables the user to take full advantage of high performance of objectives, including their parfocality. -
Page 25: Adjust The Interpupillary Distance
Chapter 4 Microscopy Procedure (Detailed) Adjust the interpupillary distance. This adjustment is to match the distance between the eyepieces with the distance between your eyes. Looking through the eyepieces, merge the right and left viewfields into one by turning the binocular part. -
Page 26: Focus On The Field Diaphragm And Center Its Position
Chapter 4 Microscopy Procedure (Detailed) Focus on the field diaphragm and center its position. (This adjustment is only for microscopes with the field diaphragm.) (1) Check that the 10x or 4x objective is in the optical path. Turn the field diaphragm ring to its right limit to stop down the diaphragm. -
Page 27: Microscopy Operations For Actual Microscopy
Chapter 4 Microscopy Procedure (Detailed) Microscopy Operations for actual microscopy Select the objective. Rotate the revolving nosepiece to the desired objective magnification. The objective will click into place when rotated into position. Every time you change the objective Click magnifications, adjust the aperture diaphragm lever according to the selected objective. -
Page 28: Adjusting The Aperture Diaphragm
Chapter 4 Microscopy Procedure (Detailed) Adjusting the Aperture Diaphragm The aperture size is increased or decreased by turning the condenser aperture diaphragm lever. If the aperture diaphragm is closed, the brightness and resolution are decreased but the contrast and range of focus are increased. If the aperture diaphragm is opened, the brightness and resolution are increased but the contrast and range of focus are decreased. -
Page 29: Focus On The Specimen
Chapter 4 Microscopy Procedure (Detailed) Focus on the specimen. (1) Looking into the eyepieces, turn the brightness control dial to adjust the brightness of the viewfield. Darkens Brightens (2) If the specimen is out of focus, rotate the focus knobs to focus on the specimen. Do NOT turn the right and left focus knobs simultaneously in opposite directions, and do NOT turn the coarse focus knob further after... -
Page 30: Adjust The Field Diaphragm
Chapter 4 Microscopy Procedure (Detailed) Adjust the field diaphragm. (This adjustment is only for microscopes with the field diaphragm.) Looking into the eyepieces, turn the field diaphragm ring until the diaphragm image just circumscribes the viewfield. Field diaphragm The field diaphragm is used to control the ring specimen's illuminated area relative to the Diaphragm... - Page 31 Chapter 4 Microscopy Procedure (Detailed) Replacing a Specimen Using the Refocusing Mechanism Try focusing on the specimen with 40x or higher magnification objective. You will find the specimen is brought very near to the objective.(*1) It will be very difficult to change the specimen without moving the focus knob.
- Page 32 Chapter 4 Microscopy Procedure (Detailed) Using the Working Distance for Focusing Each objective has its working distance indicated on its side. The working distance is the distance between the front of the objective and the specimen when the specimen image is in focus. If you have difficulties in focusing with the standard procedure described on P.17, try one of the following methods using the working distance for focusing.
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Page 33: Turn Off The Power Switch
Chapter 4 Microscopy Procedure (Detailed) Turn off the power switch. (1) Press the power switch to the “○” position. Power of the microscope will turn off and the lamp will go out. Power OFF (2) Open the claw of the specimen holder with your finger at the root or the tip tilt and remove the specimen slide. -
Page 34: Chapter 5 Miscellaneous Operations
Miscellaneous Operations Oil-Immersion Observation The “Oil” mark on the side of an objective indicates that it is an oil-immersion type objective. (The oil-immersion objective also has a black band around the barrel end.) An oil-immersion objective is used with the immersion oil applied between the front of the objective and the coverglass. - Page 35 • If you find an oil drips around the container, wipe them off. • Avoid contact of immersion oil with eyes or skin. In the event of contact with eyes or skin, take one of the following measures although Nikon immersion oil does not contain any toxic ingredients.
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Page 36: Torque Adjustment Of The Coarse Focus Knob
Chapter 5 Miscellaneous Operations 2 Torque Adjustment of the Coarse Focus Knob Torque Adjustment of the Coarse Focus Knob The tension (torque) of the coarse focus knob rotation can be adjusted. To increase the tension, turn the coarse focus knob torque adjustment ring counterclockwise. -
Page 37: Eye Level Raiser
(nominal size 2) for M4 set screws to install the raisers. Photomicrography and TV Microscopy The microscopes ECLIPSE E200 MV series and E200LED MV series can be used for photomicrography and TV microscopy. For those observations, trinocular eyepiece tube, photomicrographic equipment, TV vertical tube, and C-mount CCTV camera (optional) are available. -
Page 38: Nd Filter For The Objective
When a 3CCD TV camera is used with some relay lenses, the colors of the video image may blur. For details, contact your nearest Nikon representative. ● Color Tone The color tone of the illumination varies with the position of the brightness control dial. -
Page 39: Cord Hangers
Chapter 5 Miscellaneous Operations 7 Cord Hangers Cord Hangers Push the cord hangers (optional) into the holes on the rear of the microscope. The hangers can be used for winding the power cord around when the microscope is not in use. To remove the hangers, use a screwdriver. -
Page 40: Chapter 6 Assembly
Assembly WARNING • Before assembling or connecting devices, thoroughly read the “Safety Precautions” at the beginning of this manual, and be sure to follow all warnings and cautions written therein. • To prevent electric shock, fire, and product damage, turn off the power switches (press to the “○”... -
Page 41: Connecting The Power Cord
Chapter 6 Assembly 1 Assembly of Standard Set Removal of Shipping Clamps The stage top plate and focusing mechanism are clamped for protection against vibration and shocks during transportation. Remove these shipping clamps with the hexagonal wrench provided. • Stage Top Plate: Shipping The top plate of the stage is retained in the clamps... -
Page 42: Assembly Of Additional Components
Chapter 6 Assembly 2 Assembly of Additional Components Assembly of Additional Components Condenser The condenser is attached to the microscope before shipment. When re-attaching the condenser after removing or replacing the condenser, follow the procedure below. Lower the condenser holder to its full limit by Condenser focus knob rotating the condenser focus knob. -
Page 43: Other Accessories
Chapter 6 Assembly 2 Assembly of Additional Components (7-7) Center the field diaphragm image in the viewfield of the eyepiece by manipulating the centering screws. Centering is easier to perform if the size of the field diaphragm image is Field Field diaphragm adjusted so that it is slightly smaller... -
Page 44: Replacement Of Consumable Materials
Chapter 6 Assembly 3 Replacement of Consumable Materials Replacement of Consumable Materials Replacing the Lamp (for E200 MV series only) WARNING • To avoid electrical shock or damage to the product, turn off the power switch (press to the “○” position) and unplug the power cord before lamp replacement. - Page 45 Chapter 6 Assembly 3 Replacement of Consumable Materials Turn off the power switch (press to the “○” position) and unplug the power cord. Wait about 30 minutes until the lamp and its surroundings are cool enough to touch. Hold the field lens unit at the vertical grooves on both sides and pull it toward you to remove it.
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Page 46: Chapter 7 Optical Characteristics
Optical Characteristics Combinations of 10x (Field No. 20) Eyepiece with E-Plan Objectives Objective Total Numerical Real Depth of Resolving Working Magnification Magnification Aperture Viewfield Focus Power Distance 4× 40× 5 mm 63.2 μm 2.8 μm 30 mm 10× 100× 0.25 2 mm 10.1 μm 1.1 μm... - Page 47 Chapter 7 Optical Characteristics 2 Microscope Terminology (5) Field Number of the Eyepiece The diameter of the opening of the fieldstop inside the eyepiece measured in mm. When an eyepiece has an indication of “10x / 20”, it means that the magnification is 10x and the field number is 20 for that eyepiece.
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Page 48: Chapter 8 Troubleshooting
If you detect problems that are not listed below or the problem still persists after measures are taken, turn off the device and contact your nearest Nikon representative. Optical Dirt or dust in the viewfield. - Page 49 No immersion oil is applied to the tip of Apply Nikon immersion oil to the the oil-immersion objective. objective. (P.22) Nikon immersion oil is not used for Use Nikon immersion oil. (P.22) oil-immersion observation. Air bubbles in immersion oil. Remove bubbles. (P.22)
- Page 50 Chapter 8 Troubleshooting 1 Optical Image too bright. Causes Corrective Measures Attach the ND filter for the objective. ND filter for the objective is not used. (P.26) Adjust the voltage by rotating the Lamp voltage is too high. brightness control dial. (P.17) Insufficient brightness.
- Page 51 Chapter 8 Troubleshooting 1 Optical Darkness at the periphery, no viewfield seen, or uneven viewfield brightness. Causes Corrective Measures Revolving nosepiece is not in click-stop Revolve to click-stop position (swing the position (objective not centered in the objective correctly into the optical path). optical path).
- Page 52 Chapter 8 Troubleshooting 1 Optical Image dark on one side. (One side of the viewfield (up, down, right, or left) is not focused.) Causes Corrective Measures Revolving nosepiece is not in click-stop Revolve to click-stop position. position. Specimen rises from stage surface. Stabilize it using the holder.
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Page 53: Operational
Tip of the objectives without stopper can protection of the objective is pushed in. not be rotated. Do not use force to draw the stopper. Contact your nearest Nikon representative. High magnification objective contacts slide when changed over from low magnification. - Page 54 Chapter 8 Troubleshooting 2 Operational Binocular images not integrated. Causes Corrective Measures Interpupillary distance is not adjusted Adjust. (P.13) correctly. Diopter is not adjusted correctly. Adjust. (P.12) Excessive eye fatigue. Causes Corrective Measures Interpupillary distance is not adjusted Adjust. (P.13) correctly.
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Page 55: Electrical
Chapter 8 Troubleshooting 3 Electrical Electrical Lamp does not light when switched on. Causes Corrective Measures Insert the power cord into an electrical No electrical power. outlet. (P.29) Power cord is not connected to the Insert the power cord into the AC inlet. microscope main body. -
Page 56: Chapter 9 Care And Maintenance
• We especially recommend that the objectives and eyepieces be kept in a container (such as a desiccator) with desiccant in it. Periodical Inspections (Charged) • To maintain the performance of the microscope, periodical inspections and maintenance are recommended. • For details, contact your nearest Nikon representative. -
Page 57: Chapter 10 Specifications
Specifications Microscopy (Principles) Use the objective and eyepieces of the microscope to magnify minute cells and tissue optically, manipulate the lever and handle of the microscope unit to precisely focus or move the observation point. Then observe or shoot the sample fixed on the slide. •... - Page 58 Chapter 10 Specifications 2 Performance Properties (4) Stage: Stroke: X axis: 77 mm Y axis: 53 mm (5) Revolving 4-hole fixed type Nosepiece: (6) Illumination • E200 MV Series Light Source: Lamp Rating: 6 V-30 W halogen lamp (PHILIPS 5761) Average lamp lifetime: 100hrs Output Rating: 6 V 5 A max.
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Page 59: Physical Properties
Chapter 10 Specifications 3 Physical Properties Physical Properties (1) Model Name: Microscope Main Body • E200 MV Series ECLIPSE E200 MV L (Stage knob left side, without field diaphragm) ECLIPSE E200 MV LS (Stage knob left side, with field diaphragm) ECLIPSE E200 MV R (Stage knob right side, without field diaphragm) ECLIPSE E200 MV RS (Stage knob right side, with field diaphragm) •... - Page 60 Chapter 10 Specifications 3 Physical Properties (5) Safety Standards Compliance • UL listed product. (C-UL US Listing Mark approved) • This product meets FCC Part 15 Subpart B Class A requirements: This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.
- Page 61 This symbol is provided for use in the People’s Republic of China, for environmental protection in the fi elds of electronic information products.
- Page 62 1300 Walt Whitman Road, Melvile, N.Y. 11747-3064, U.S.A. 1300 Walt Whitman Road, Melvile, N.Y. 11747-3064, U.S.A. tel. +1-631-547-8500 tel. +1-631-547-8500 NIKON INSTRUMENTS EUROPE B.V. NIKON INSTRUMENTS EUROPE B.V. Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlands Laan van Kronenburg 2, 1183 AS Amstelveen, The Netherlands tel.
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