Labconco Protector 50600 User Manual page 37

 Perform evacuation and fill cycle on the transfer chamber using the vacuum
outlet and gas inlet valves. Depending on the vacuum level achieved, it
takes 1-5 cycles to reduce oxygen and moisture levels below 1%. Porous
materials being transferred may require longer sustained vacuum purges to
remove the oxygen and moisture trapped in the materials.
 After transferring materials to the main glove box chamber, perform the
required evacuation and fill cycles (1-499) on the main chamber using the
vacuum outlet and gas inlet valves. Normally 75-100 evacuation and fill
cycles will reduce moisture and oxygen levels below 1% (see Appendix D).
An additional 100 cycles will reduce moisture and oxygen levels below
0.3% or 3,000 ppm. The use of the accessory AtmosPure Re-Gen Gas
Purifier can now be turned on. The use of external glove port covers will
expedite the evacuation and fill cycle times and help maintain low
moisture and oxygen levels when the glove box is not in use.
 Maintaining a slight main chamber pressure difference of -1" to +1" water
gauge can help to preserve the glove box atmosphere. The moisture/
oxygen permeation is reduced at slight pressure differences.
 The AtmosPure Re-Gen Gas Purifier is required to further reduce and
maintain oxygen and moisture levels below 0.3%. The AtmosPure Re-
Gen Gas Purifier can maintain oxygen levels below 1 ppm and
moisture levels below 1 ppm.
 Monitoring moisture and oxygen levels inside the glove box is
recommended by using accessory monitors (see Chapter 7).
Work Techniques
 Segregate all clean and contaminated materials in the work area.
 Maintain work at a slightly negative pressure if working with hazardous
compounds.
 Continuously run the AtmosPure Re-Gen Gas Purifier to preserve the inert
atmosphere if so desired.
Transferring Materials to the Outside
 All open chemical weigh vessels or containers must be sealed before
removing materials from the glove box main chamber.
 Upon completion of work, the main glove box chamber must be evacuated
and filled approximately 5-10 times to remove airborne contaminants from
the work area. The exact number of cycles should be determined by your
Safety Officer.
Product Service 1-800-522-7658
Chapter 5: Using Your Glove Box
33