Labconco Protector 50600 User Manual page 27

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Results were attained at Labconco Corporation under controlled conditions. Actual results may vary.
The validation requirements and required testing instruments for all applications
shall be determined by the end user and Safety Officer. Other validation test
methods such as pressure leak decay tests may be conducted per ISO 10648-2
standards. The pressure leak decay test may be performed, but the pressure inside
the glove box must be adjusted accurately for temperature and barometric pressure.
These pressure leak decay tests are conducted under negative pressure of
-250 Pa (-1" water gauge) for operational use or -1000 Pa (-4" water gauge) for the
acceptance test; the pressure leak decay tests are usually performed under stable
barometric pressure and temperature conditions to minimize the effect of these
variables.
A 0.1°C fall in temperature results in an equivalent pressure change of -34 Pa
or -0.14 inches of water gauge. A 0.01 inches of mercury rise in barometric
pressure results in an equivalent pressure change of -34 Pa or
-0.14 inches of water gauge.
Product Service 1-800-522-7658
Protector Fiberglass CA Glove Box
Oxygen Permeation at -4" H2O
ISO 10648-2 Test Method
10 15 20
Oxygen ppm
Chapter 3: Getting Started
25 30
Figure 3-10
Barometric Pressure
29.61 inches of Hg
Main Chamber
Pressure: -4 Inches
H O
2
Transfer Chamber
Pressure: 0 inches of
Hg
Leak Rate Box
5
8.7x10¯
Class 1 < 0.0005
Permeation
0.30ppm/min
35 40
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