Thermo Scientific VH-D20 Operating Manual page 94

6 • Operation
Before Starting Sample Analysis
System Equilibration
Page 94
Before starting an analysis:
• Check the liquid level in the solvent reservoirs. Verify that the
amount of solvent is sufficient for the analysis.
• Close the doors of all modules in the Vanquish system, if not already
done.
• Make sure that the pump flow rate that is delivered to the charged
aerosol detector is within the specified range. See
Specifications (} page 170).
• To deliver pump flow to the charged aerosol detector, follow these
steps:
a) Turn on the detector gas flow. See
Gas Flow (} page 102). The pump flow must be turned off. Wait
at least 5 minutes before you continue.
b) Turn on the pump flow to the detector. Have the pump deliver
flow to the detector.
• Make sure that the chromatography system is properly equilibrated
(see further down).
System equilibration should include the following operations:
• Purging the pump (all channels, including those not used for the
application)
• Flushing the entire chromatography system with the starting solvent
to rinse out any solvent from a previous analysis run
• Warming up (or cooling down) all temperature-controlled devices in
the system to the starting temperature. Temperature-controlled
devices can be, for example
Column compartment and post-column cooler
¨
Sample compartment thermostatting in the autosampler
¨
Flow cell in a fluorescence detector
¨
Evaporation tube in a charged aerosol detector
¨
• Turning on the lamp (or lamps) in the UV/VIS detector
• Monitoring the pump pressure and pressure ripple and checking
that the pressure is stable and the ripple within reasonable limits for
the application
Turning On/Off the Detector
Charged Aerosol Detectors (VH-D20, VF-D20)
Performance
Operating Manual