Various Observation Methods; Transmitted Phase Contrast Observation Method - Olympus CX43 Instructions Manual

5

Various observation methods

This section describes the procedures of observation methods other than brightfield/darkfield observations. For brightfield and
darkfield observations, see "3 Transmitted brightfield/darkfield observation methods".

5-1 Transmitted phase contrast observation method

For the phase contrast observation, the objective for phase contrast and the centering telescope (CT-5) are necessary.
If the analyzer for simple polarization and the polarizer are engaged in the light path, remove them from the light path.
Set the main switch to (ON).
Select BF (brightfield observation).
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen into focus and adjust the brightness.
Engage the objective for phase contrast in the light path.
Select either Ph1, Ph2 or Ph3 (phase contrast observation).
Bring the specimen into focus.
Observation
Adjust the interpupillary
distance.
Adjust the diopter.
Center the ring slit.
Place the necessary filter.
Adjust the brightness.
(Operation portion)
1
Main switch
2
Condenser turret
3
Specimen holder
4
X-axis/Y-axis knobs
5
Revolving nosepiece
6
Coarse/fine focusing knobs
7
Brightness adjustment knob
8
Binocular portion
9
Diopter adjustment ring
5
Revolving nosepiece
2
Condenser turret
a
Ring slit centering knob
6
Coarse/fine focusing knobs
b
Various filters
7
Brightness adjustment knob
CX43
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