Olympus BXFM Instructions Manual page 59

Phenomena
j) The one-sided blur appears in
the observation image.
k) The observation image shifts
when defocusing.
j) During the fluorescence
observation, the background
becomes brighter.
Cause
The nosepiece is not attached
correctly.
The objective is not correctly
engaged in the light path.
The sample is not parallel to the
stage.
The sample exceeds the specified
weight.
The nosepiece is not attached
correctly.
The objective is not correctly
engaged in the light path.
The aperture diaphragm is narrowed
down the centering is not performed
yet.
This is caused by the in-house
fluorescence of the transmitted light
illumination optical systems.
Remedy
Push in the nosepiece along the
mounting dovetail until it touches the
end, and secure it.
Turn the nosepiece until the clicking
sound is heard and engage the
objective in the light path.
Correct the sample to parallel to the
stage or change the sample.
Replace with another sample.
Push in the nosepiece along the
mounting dovetail until it touches the
end, and secure it.
Turn the nosepiece until the clicking
sound is heard and engage the
objective in the light path.
Perform the centering of the aperture
diaphragm.
Insert the light shield sheet in the
space of the stage.
BXFM
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