Agilent Technologies InfinityLab LC Series User Manual page 169

5 Optimizing Performance
Optimization for Lowest Carryover
Using an Injector Program
The process is based on a program that switches the bypass grove of the
injection valve into the flow line for cleaning. This switching event is performed at
the end of the equilibration time to ensure that the bypass grove is filled with the
start concentration of the mobile phase. Otherwise the separation could be
influenced, especially if microbore columns are used.
Example
Outside wash of needle in vial p2-A1 before injection
Injector program:
•
•
•
Overlapped injection together with additional injection valve switching is not
NOTE
possible.
Agilent InfinityLab LC Series Vialsamplers User Manual
Draw x.x (y) µl from sample
WASH vial location p2-A1
Inject
Wait (equilibration time - see text above)
Valve bypass
Wait 0.2 min
Valve mainpass (main path)
Valve bypass
Valve mainpass (main path)
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