Page 1 Agilent InfinityLab LC Series Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 2: Warranty
Copyright adhered to, could result in damage manual and any information contained herein, including but not limited to the © Agilent Technologies, Inc. to the product or loss of important implied warranties of merchantability 2016-2024 data. Do not proceed beyond a and fitness for a particular purpose.
Page 3: Table Of Contents
Contents In This Book 7 Introduction 8 Overview of the Module 9 Product Description of the 1260 Infinity III Diode Array Detector WR (G7115A) 10 Features of the 1260 Infinity III Diode Array Detector WR (G7115A) 11 Product Description of the 1260 Infinity III Multiple Wavelength Detector (G7165A) 12 Features of the 1260 Infinity III Multiple Wavelength Detector (G7165A) 13 Operating Principle 14...
Page 4 Optimizing the Performance of the Module 75 Introduction 76 Optimization Overview 77 Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 79 Optimizing Selectivity 89 Optimizing the Detector Regarding to the System 91 Warm up of the Detector 97 Diagnostics and Troubleshooting 99 Diagnostic Features 100 Overview of Available Tests and Tools 101 Maintenance and Troubleshooting Tools of the Module 102 Agilent Lab Advisor Software 140 Other Lab Advisor Functions 141...
Page 5 Correcting Leaks 225 Replace Leak Handling System Parts 228 Replace the Module Firmware 230 Information from Module’s Assemblies 232 Parts and Materials for Maintenance 233 Overview of Maintenance Parts 234 Standard Flow Cell 236 Standard Flow Cell Bio-inert 238 Semi-Micro Flow Cell 240 Micro Flow Cell 242 High Pressure Flow Cell 244 Prep Flow Cell - SST 246 Prep Flow Cell - Quartz 248 Nano Flow Cells 250...
Page 6 Material Information 317 At-a-Glance Details About Agilent Capillaries 325 Waste Electrical and Electronic Equipment (WEEE) Directive 329 Radio Interference 330 RFID Statement 331 Sound Emission 333 UV-Radiation 334 Declaration of Conformity for HOX2 Filter 335 Agilent Technologies on Internet 337 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 7: In This Book
9007202612246027 ag-textnode-base-id: 3357505035 In This Book This manual covers the following Agilent InfinityLab LC Series modules: • Agilent 1260 Infinity III DAD WR (G7115A) • Agilent 1260 Infinity III MWD (G7165A) Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 8: Introduction
ag-textnode-version-id: 18014402492107787 ag-textnode-base-id: 3982625803 Introduction This chapter gives an introduction to the module and instrument overview. Overview of the Module 9 Product Description of the 1260 Infinity III Diode Array Detector WR (G7115A) 10 Features of the 1260 Infinity III Diode Array Detector WR (G7115A) 11 Product Description of the 1260 Infinity III Multiple Wavelength Detector (G7165A) 12 Features of the 1260 Infinity III Multiple Wavelength Detector (G7165A) 13...
Page 9: Overview Of The Module
Introduction Overview of the Module ag-textnode-version-id: 9007202613040139 ag-textnode-base-id: 3358299147 Overview of the Module The detector described in this manual is designed for highest optical performance, GLP compliance and easy maintenance. It includes the following features: • 120 Hz data acquisition rate for (ultra-) fast LC applications, •...
Page 10: Product Description Of The 1260 Infinity Iii Diode Array Detector Wr (G7115A)
Introduction Product Description of the 1260 Infinity III Diode Array Detector WR (G7115A) ag-textnode-version-id: 9007202613049099 ag-textnode-base-id: 3358308107 Product Description of the 1260 Infinity III Diode Array Detector WR (G7115A) The Agilent 1260 Infinity III DAD WR Detector is designed for highest optical performance, GLP compliance and easy maintenance. With its 120 Hz data acquisition rate the detector is perfectly suited for fast LC applications.
Page 11: Features Of The 1260 Infinity Iii Diode Array Detector Wr (G7115A)
Introduction Features of the 1260 Infinity III Diode Array Detector WR (G7115A) ag-textnode-version-id: 3358312587 ag-textnode-base-id: 3358312587 Features of the 1260 Infinity III Diode Array Detector WR (G7115A) • Higher sensitivity and selectivity - simultaneous detection of up to eight compound-specific wavelengths. • Low detection limits - low noise front-end electronics and the patented flow cell design delivers very low detection limits thanks to the minimization of short-term noise (<...
Page 12: Product Description Of The 1260 Infinity Iii Multiple Wavelength Detector (G7165A)
Introduction Product Description of the 1260 Infinity III Multiple Wavelength Detector (G7165A) ag-textnode-version-id: 9007202613058571 ag-textnode-base-id: 3358317579 Product Description of the 1260 Infinity III Multiple Wavelength Detector (G7165A) The 1260 Infinity III Multiple Wavelength Detector is designed for highest optical performance, GLP compliance, and easy maintenance. With its 120 Hz data acquisition rate, the detector is perfectly suited for fast LC applications.
Page 13: Features Of The 1260 Infinity Iii Multiple Wavelength Detector (G7165A)
Introduction Features of the 1260 Infinity III Multiple Wavelength Detector (G7165A) ag-textnode-version-id: 3358322059 ag-textnode-base-id: 3358322059 Features of the 1260 Infinity III Multiple Wavelength Detector (G7165A) • Higher sensitivity and selectivity - simultaneous detection of up to eight compound-specific wavelengths. • Low detection limits - low noise front-end electronics and the patented flow cell design delivers very low detection limits thanks to the minimization of short-term noise (<...
Page 14: Operating Principle
Introduction Operating Principle ag-textnode-version-id: 3982632075 ag-textnode-base-id: 3982632075 Operating Principle ag-textnode-version-id: 3358294667 ag-textnode-base-id: 3358294667 Optical System The optical system of the detector is shown in Figure below. Its illumination source is a combination of a deuterium-arc-discharge lamp for the ultraviolet (UV) wavelength range and a tungsten lamp for the visible (VIS) and short-wave near-infrared (SWNIR) wavelength range.
Page 15 Introduction Operating Principle Lamps The light source for the UV-wavelength range is a deuterium lamp with a shine- through aperture. As a result of plasma discharge in low-pressure deuterium gas, the lamp emits light over the 190 nm to approximately 800 nm wavelength range. The light source for the visible and SWNIR wavelength range is a low noise tungsten lamp.
Page 16 Introduction Operating Principle Diode Array The diode array is a series of 1024 individual photodiodes and control circuits located on a ceramic carrier. With a wavelength range from 190 – 950 nm the sampling interval is < 1 nm. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 17: Site Requirements And Specifications
ag-textnode-version-id: 27021601747014155 ag-textnode-base-id: 3982791179 Site Requirements and Specifications This chapter provides information on environmental requirements, physical and performance specifications. Site Requirements 18 Power Considerations 18 Power Cords 19 Bench Space 20 Environment 20 Specifications of the 1260 Infinity III Diode Array Detector WR (G7115A) 22 Specifications of the 1260 Infinity III Multiple Wavelength Detector (G7165A) 25 Specification Conditions 28 Time constant versus response time 28...
Page 18: Site Requirements
Site Requirements and Specifications Site Requirements ag-textnode-version-id: 3357613323 ag-textnode-base-id: 3357613323 Site Requirements A suitable environment is important to ensure optimal performance of the instrument. ag-textnode-version-id: 3358327051 ag-textnode-base-id: 3358327051 Power Considerations The module power supply has wide ranging capability. It accepts any line voltage in the range described in Physical Specifications.
Page 19: Power Cords
— Never use the power cords that Agilent Technologies supplies with this instrument for any other equipment. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 20: Bench Space
Site Requirements and Specifications Site Requirements Electrical shock hazard WARNING ag-struc-frag-safety-version-id: 3003281291 ag-struc-frag-safety-base-id: 3003281291 Solvents may damage electrical cables. — Prevent electrical cables from getting in contact with solvents. — Exchange electrical cables after contact with solvents. ag-textnode-version-id: 3431204875 ag-textnode-base-id: 3431204875 Bench Space The module dimensions and weight (see Physical Specifications) allow you to place the module on almost any desk or laboratory bench.
Page 21 Site Requirements and Specifications Site Requirements The module is designed to operate in a typical electromagnetic environment NOTE (EN61326-1) where RF transmitters, such as mobile phones, should not be used in close proximity. Condensation within the module CAUTION ag-struc-frag-safety-version-id: 3003284747 ag-struc-frag-safety-base-id: 3003284747 Condensation can damage the system electronics.
Page 22: Specifications Of The 1260 Infinity Iii Diode Array Detector Wr (G7115A)
Site Requirements and Specifications Specifications of the 1260 Infinity III Diode Array Detector WR (G7115A) ag-textnode-version-id: 18014404588736779 ag-textnode-base-id: 6079254795 Specifications of the 1260 Infinity III Diode Array Detector WR (G7115A) Table 1: Physical specifications of the 1260 Infinity III Diode Array Detector WR (G7115A) Parameter Name External Value Comment...
Page 23 Site Requirements and Specifications Specifications of the 1260 Infinity III Diode Array Detector WR (G7115A) Type Specification Drift < 0.9·10 AU/h at 254 nm and 750 nm Linear absorbance range > 2 AU (5 %) at 273 nm Wavelength range 190 – 950 nm Wavelength accuracy ± 1 nm, self-calibration with deuterium lines, verification with holmium oxide filter Wavelength bunching 1 –...
Page 24 Site Requirements and Specifications Specifications of the 1260 Infinity III Diode Array Detector WR (G7115A) Type Specification Instrument control LC and CE Drivers A.02.14 or above Instrument Control Framework (ICF) A.02.04 or above Lab Advisor B.02.08 or above InfinityLab Assist (G7180A) with firmware D.07.40 or above Agilent Instant Pilot (G4208A) with firmware B.02.20 or above For details about supported software versions refer to the compatibility matrix of your version of the LC and CE Drivers.
Page 25: Specifications Of The 1260 Infinity Iii Multiple Wavelength Detector (G7165A)
Site Requirements and Specifications Specifications of the 1260 Infinity III Multiple Wavelength Detector (G7165A) ag-textnode-version-id: 18014404588740875 ag-textnode-base-id: 6079258891 Specifications of the 1260 Infinity III Multiple Wavelength Detector (G7165A) Table 3: Physical specifications of the 1260 Infinity III Multiple Wavelength Detector (G7165A) Parameter Name External Value Comment Weight 12 kg (26.5 lbs)
Page 26 Site Requirements and Specifications Specifications of the 1260 Infinity III Multiple Wavelength Detector (G7165A) Type Specification Short term signal noise < ± 0.7·10 AU at 254 nm and 750 nm (ASTM) Drift < 0.9·10 AU/h at 254 nm Linear absorbance range > 2 AU (5 %) at 273 nm Wavelength range 190 –...
Page 27 Site Requirements and Specifications Specifications of the 1260 Infinity III Multiple Wavelength Detector (G7165A) Type Specification Instrument control LC and CE Drivers A.02.14 or above Instrument Control Framework (ICF) A.02.04 or above InfinityLab Assist (G7180A) with firmware D.07.40 or above Agilent Instant Pilot (G4208A) B.02.20 or above Lab Advisor B.02.08 or above For details about supported software versions refer to the compatibility matrix of your version of the LC and CE Drivers.
Page 28: Specification Conditions
Site Requirements and Specifications Specification Conditions ag-textnode-version-id: 9007202686057995 ag-textnode-base-id: 3431317003 Specification Conditions Following many of the principles outlined in ASTM method E165798. Reference conditions: cell path length 10 mm, wavelength 254 and 750 nm with reference wavelength 360 nm/100 nm, slit width 4 nm, time constant 2 s (equal to response time 4 s), flow 1 mL/min LC-grade Methanol.
Page 29: Installation
ag-textnode-version-id: 9007205334142987 ag-textnode-base-id: 6079401995 Installation The installation of the module will be done by an Agilent service representative. In this chapter, only installation of user-installable options and accessories are described. Installing Capillaries 30 Install Capillaries 30 Install Stainless Steel Clad PEEK Capillaries 34 Handling Leak and Waste 40 Drain Connectors Installation 43 Waste Concept 48...
Page 30: Installing Capillaries
Installation Installing Capillaries ag-textnode-version-id: 45036000351652875 ag-textnode-base-id: 4077947915 Installing Capillaries This section provides information on how to install capillaries and fittings. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 31: Install Capillaries
Installation Installing Capillaries ag-textnode-version-id: 9007202257747211 ag-textnode-base-id: 3003006219 Install Capillaries Capillaries and connections depend on which system is installed. As you move to smaller-volume, high-efficiency columns, you will want to use NOTE narrow id tubing, as opposed to the wider id tubing used for conventional HPLC instruments.
Page 32 Installation Installing Capillaries From 5500-1264 (Capillary Ti 0.17 mm x 500 mm, SL/SLV) Pump Multisampler G5667-81005 (Capillary PK/ST 0.17 mm x 500 mm, RLO/RLO Multisampler (Bio-inert)) 5067-4741 (ZDV union (Bio-inert)) Capillary Bio-inert Heat Exchanger G7116-60041 (Quick Connect Heat Exchanger Bio-inert) 0890-1763 (Capillary PEEK 0.18 mm x 1.5 m) and Column/MCT Valve Detector 5063-6591 (PEEK Fittings 10/PK) 5062-8535 (Waste accessory kit (Flow Cell to waste)) Waste 5062-2462 (Tube PTFE 0.7 mm x 5 m, 1.6 mm od)
Page 33 Installation Installing Capillaries 3 Carefully slide the ferrule components on after the nut and then finger-tighten the assembly while ensuring that the tubing is completely seated in the bottom of the end fitting. 4 Use a stable port installed to the module to gently tighten the fitting facing to the module.
Page 34: Install Stainless Steel Clad Peek Capillaries
Installation Installing Capillaries ag-textnode-version-id: 27021601122486155 ag-textnode-base-id: 3358263179 Install Stainless Steel Clad PEEK Capillaries This installation procedure applies for capillaries and corresponding fittings used NOTE in modules delivered before January 2013. The 1260 Infinity Bio-inert LC system uses PEEK capillaries that are clad with stainless steel.
Page 35: First Step: Finger-Tight Fitting
Installation Installing Capillaries ag-textnode-version-id: 3358267403 ag-textnode-base-id: 3358267403 First Step: Finger-tight Fitting 1 Tighten the fitting using your fingers. ag-textnode-version-id: 3804109323 ag-textnode-base-id: 3804109323 Second Step: Installation to Connector In the second step (Second Step: Installation to Hard Connectors on page 35 or Second Step: Installation to Soft Connectors on page 36), a wrench is used to rotate the fitting relative to the finger-tight position by a defined angle.
Page 36: Second Step: Installation To Hard Connectors
Installation Installing Capillaries ag-textnode-version-id: 3358271115 ag-textnode-base-id: 3358271115 Second Step: Installation to Hard Connectors Use this procedure for hard connectors made from metal (titanium) or ceramics. In the system, these are connections to and from the analytical head of the autosampler (connections to injection valve and needle), and to a metal column. First installation of a capillary to a hard connector 1 When tightening a fitting for the first time, start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by...
Page 37: Second Step: Installation To Soft Connectors
Installation Installing Capillaries ag-textnode-version-id: 3358274827 ag-textnode-base-id: 3358274827 Second Step: Installation to Soft Connectors Use this procedure for soft connectors, which are typically made from PEEK. These are the following connections: • to and from all bio-inert valves (injection valve in the autosampler and valves in the thermostatted column compartment and 1290 Infinity Valve Drive), •...
Page 38 Installation Installing Capillaries 1 When tightening the fitting for the second and subsequent times, again start from the finger-tight position (which is not necessarily a vertical wrench position) and rotate the wrench by 135 – 180 °. Staying below 135 ° (grey arrow) could be insufficiently tight enough, more than 180 ° (red arrow) could damage the capillary.
Page 39: Removing Capillaries
Installation Installing Capillaries ag-textnode-version-id: 3358286731 ag-textnode-base-id: 3358286731 Removing Capillaries Potential damage of capillaries CAUTION ag-struc-frag-safety-version-id: 3359881867 ag-struc-frag-safety-base-id: 3359881867 — Do not remove fittings from used capillaries. To keep the flow path free of stainless steel, the front end of the capillary is made of PEEK.
Page 40: Handling Leak And Waste
36028800545451531 ag-textnode-base-id: 3526487563 Handling Leak and Waste The Agilent InfinityLab LC Series has been designed for safe leak and waste handling. It is important that all security concepts are understood and instructions are carefully followed. The solvent cabinet is designed to store a maximum volume of 8 L solvent. The maximum volume for an individual bottle stored in the solvent cabinet should not exceed 2 L.
Page 41 Installation Handling Leak and Waste Figure 5: Infinity III Leak Waste Concept (Flex Bench installation) Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 42 Installation Handling Leak and Waste Figure 6: Infinity III Single Stack Leak Waste Concept (bench installation) Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 43 Installation Handling Leak and Waste Figure 7: Infinity III Two Stack Leak Waste Concept (bench installation) The waste tube connected to the leak plane outlet on each of the bottom instruments guides the solvent to a suitable waste container. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 44: Drain Connectors Installation
Installation Handling Leak and Waste ag-textnode-version-id: 9007209510841867 ag-textnode-base-id: 10256100875 Drain Connectors Installation Drain Connectors have been developed to improve leak drainage for low flow leaks of high viscosity solvents (for example, isopropanol) in Agilent InfinityLab LC Series Systems. Install these parts to modules where they are missing (usually preinstalled).
Page 45 Installation Handling Leak and Waste Table 8: Compatibility of drain connectors and modules Drain Connector Type Compatible Module Compatible Module Type Double G7116A/B Column Compartment Single G7114A/B Detector G7115A G7117A/B/C G7121A/B G7162A/B G7165A G7129A/B/C Sampler G7167A/B/C G5668A G7137A G7157A G4767A G7122A Degasser G7104A/C Pump...
Page 46 Installation Handling Leak and Waste In case of incorrect installation, drain connectors cannot fully perform the NOTE intended function. It is not required to power off the HPLC stack to install Single and Double Drain NOTE Connectors. The installation of the connectors does not affect the analysis performed during the installation.
Page 47 Installation Handling Leak and Waste 1 Align the ring with the leak drain outlet of the module, press slightly with the fingers, and slide the connector along the leak drain outlet until it is aligned with the front of the leak drain. Make sure that the following requirements are covered: •...
Page 48: Waste Concept
Installation Handling Leak and Waste ag-textnode-version-id: 9007202332686987 ag-textnode-base-id: 3077945995 Waste Concept Agilent recommends using the 5043-1221 (6 L waste can with 1 Stay Safe cap GL45 with 4 ports) for optimal and safe waste disposal. If you decide to use your own waste solution, make sure that the tubes don't immerse in the liquid. ag-textnode-version-id: 3596422027 ag-textnode-base-id: 3596422027 Waste Guidance...
Page 49: Leak Sensor
Installation Handling Leak and Waste The waste drainage must go straight into the waste containers. The waste flow NOTE must not be restricted at bends or joints. ag-textnode-version-id: 3002300811 ag-textnode-base-id: 3002300811 Leak Sensor Solvent incompatibility CAUTION ag-struc-frag-safety-version-id: 3004406283 ag-struc-frag-safety-base-id: 3004406283 The solvent DMF (dimethylformamide) leads to corrosion of the leak sensor.
Page 50: Connecting Modules And Control Software
WARNING ag-struc-frag-safety-version-id: 3822076043-1 ag-struc-frag-safety-base-id: 3822076043 Using cables not supplied by Agilent Technologies can lead to damage of the electronic components or personal injury. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 51: Instrument Configuration
Installation Instrument Configuration ag-textnode-version-id: 3283594379 ag-textnode-base-id: 3283594379 Instrument Configuration Example shows an instrument configuration with a Diode Array Detector. 1 Set the switches of the Configuration switch at the rear of the module: a All switches DOWN: module uses the default IP address 192.168.254.11. b Switch 4 UP and others DOWN: module uses DHCP.
Page 52 Installation Instrument Configuration b Lab Advisor (Instrument Overview - Add Instrument): Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 53: Using The Module
ag-textnode-version-id: 18014404592921483 ag-textnode-base-id: 6083439499 Using the Module This chapter provides information on how to use the module. General Information 54 Turn On/Off 54 Status Indicators 56 Preparation of the System 58 Prepare a Run 58 Prime and Purge the System 65 Preparing the Detector 67 Preparing the Module 68 The Detector User Interface 68 Detector Control Settings 70 Method Parameter Settings 71...
Page 54: General Information
Using the Module General Information ag-textnode-version-id: 36028801094302731 ag-textnode-base-id: 4075338763 General Information ag-textnode-version-id: 18014401511325195 ag-textnode-base-id: 3001843211 Turn On/Off This procedure exemplarily shows an arbitrary LC stack configuration. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 55 Using the Module General Information 2 On/Off switch: On 3 Turn instrument On/Off with the control software. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 56: Status Indicators
Using the Module General Information 4 On/Off switch: Off ag-textnode-version-id: 36028800065377547 ag-textnode-base-id: 3046413579 Status Indicators The module status indicator indicates one of six possible module conditions. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 57 Using the Module General Information Figure 9: Arbitrary LC stack configuration (example) Idle Run mode Not-ready. Waiting for a specific pre-run condition to be reached or completed. Error mode - interrupts the analysis and requires attention (for example, a leak or defective internal components).
Page 58: Preparation Of The System
Using the Module Preparation of the System ag-textnode-version-id: 63050398769392779 ag-textnode-base-id: 3986205835 Preparation of the System ag-textnode-version-id: 18014403027169547 ag-textnode-base-id: 4517687563 Prepare a Run This procedure exemplarily shows how to prepare a run. Parameters as shown in the screenshots may vary, depending on the system installed. Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3004430475-1...
Page 59 Using the Module Preparation of the System 1 Switch on the detector. 2 Fill the solvent bottles with adequate solvents for your application. 3 Place solvent tubings with bottle head assemblies into the solvent bottles. 4 Place solvent bottles into the solvent cabinet. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 60 Using the Module Preparation of the System 5 Solvent bottle filling dialog (in the software). Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 61 Using the Module Preparation of the System 6 Purge the pump. For details on priming and purging, refer to the technical note Best Practices for NOTE Using an Agilent LC System Technical Note (InfinityLab-BestPractice-en- SD-29000194.pdf, SD-29000194). 7 Change solvent type if necessary. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 62 Using the Module Preparation of the System 8 Choose the tray format of the sampler. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 63 Using the Module Preparation of the System 9 Add a new column. 10 Enter the column information. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 64 Using the Module Preparation of the System 11 Select the column in the Method settings of the column compartment. 12 Set the detector parameters according to the needs of your method. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 65 Using the Module Preparation of the System Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 66: Prime And Purge The System
Using the Module Preparation of the System ag-textnode-version-id: 9007202538343307 ag-textnode-base-id: 3283602315 Prime and Purge the System When the solvents have been exchanged or the pumping system has been turned off for a certain time (for example, overnight) oxygen will re-diffuse into the solvent channel between the solvent reservoir, vacuum degasser (when available in the system) and the pump.
Page 67: Preparing The Detector
Using the Module Preparation of the System ag-textnode-version-id: 3358396299 ag-textnode-base-id: 3358396299 Preparing the Detector For best performance of the detector • Let the lamp warm-up and stabilize for at least one hour (initial turn on of the module requires a longer time depending on the environment and the application needs);...
Page 68: Preparing The Module
Using the Module Preparing the Module ag-textnode-version-id: 18014402495152267 ag-textnode-base-id: 3985670283 Preparing the Module ag-textnode-version-id: 9007202614428171 ag-textnode-base-id: 3359687179 The Detector User Interface Within the detector GUI, there are active areas. If you move the mouse cursor across the icons the cursor will change. 1.
Page 69 Using the Module Preparing the Module EMF Status shows Run / Ready / Error state and “Not Ready text” or “Error text” • Offline (gray) • Ok. No Maintenance required (green) • EMF warning. Maintenance might be required (yellow) • EMF warning. Maintenance required (red) Important: The EMF settings can be accessed via Agilent Lab Advisor.
Page 70: Detector Control Settings
Using the Module Preparing the Module ag-textnode-version-id: 3359691659 ag-textnode-base-id: 3359691659 Detector Control Settings The figure shows the default settings. • Lamps: can be turned ON/OFF. • At Power On: automatic lamp-on at power on. • Analog Output Range: can be set to either 100 mV or 1 V full scale, for additional settings see Analog Output (under Method Parameter Settings...
Page 71: Method Parameter Settings
Using the Module Preparing the Module ag-textnode-version-id: 9007202614437131 ag-textnode-base-id: 3359696139 Method Parameter Settings These settings are available via Menu > Instrument > Set up Instrument Method or via right click into the module’s active area (does not show the Instrument Curves tab). Figure 10: Method parameter settings For additional help and support: Highlight the desired cell and press F1.
Page 72 Using the Module Preparing the Module Table 10: Method Parameter Settings Signals Up to 8 individual signals can be set. For each of the signals, the wavelength and bandwidth can be set for sample and reference. Limits: • Wavelength: 190.0 to 950.0 nm in steps of 0.1 nm •...
Page 73 Using the Module Preparing the Module Spectrum Settings (DAD only) • Store: None / All ALL spectra are taken continuously depending on the setting of the Peakwidth. Eight spectra are acquired per Peakwidth. The acquisition time for one spectrum is slightly less than the Peakwidth divided by 8, that is, greater than or equal to 0.01 s and less than or equal to 2.55 s.
Page 74 Using the Module Preparing the Module Timetable You may set up time events to change functions with their parameters over the run time. Add lines as required. Time Limits: 0.00 to 99999.00 min in steps of 0.01 min. Via the buttons in the bottom area, time table lines can be added, removed, cut copied, pasted or completely cleared.
Page 75: Optimizing The Performance Of The Module
ag-textnode-version-id: 18014402569652619 ag-textnode-base-id: 4060170635 Optimizing the Performance of the Module This chapter provides information on how to optimize the module. Introduction 76 Optimization Overview 77 Choosing a Flow Cell 78 Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 79 Flow Cell Path Length 79 Peak Width (Response Time) 81 Sample and Reference Wavelength and Bandwidth 82 Slit Width 85 Optimizing Spectral Acquisition 87...
Page 76: Introduction
Optimizing the Performance of the Module Introduction ag-textnode-version-id: 3358154891 ag-textnode-base-id: 3358154891 Introduction The detector has a variety of parameters that can be used to optimize performance. Depending on whether signal or spectral data need to be optimized, different settings are recommended. The following sections describe optimization for: •...
Page 77: Optimization Overview
Optimizing the Performance of the Module Optimization Overview ag-textnode-version-id: 3358159371 ag-textnode-base-id: 3358159371 Optimization Overview Table 11: Optimization Overview Parameter Impact 1 Selection of flow cell • peak resolution versus sensitivity • Choose flow cell according to used column, see Figure 11 on page 78.
Page 78: Choosing A Flow Cell
Optimizing the Performance of the Module Optimization Overview Parameter Impact • Use 4 nm slit for normal applications. • spectral resolution, sensitivity and • Use narrow slit (e.g 1 nm) if your analytes have narrow absorbance bands linearity. and for high concentrations. •...
Page 79: Optimizing For Sensitivity, Selectivity, Linearity And Dispersion
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3357544075 ag-textnode-base-id: 3357544075 Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3358186379 ag-textnode-base-id: 3358186379 Flow Cell Path Length Lambert-Beer’s law shows a linear relationship between the flow cell path length and absorbance.
Page 80 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Analysis of pesticide standard 6-mm optical path length 10-mm optical path length Time (min) Figure 12: Influence of Cell Path Length on Signal Height Traditionally LC analysis with UV detectors is based on comparing measurements with internal or external standards.
Page 81: Peak Width (Response Time)
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3358190859 ag-textnode-base-id: 3358190859 Peak Width (Response Time) Response time describes how fast the detector signal follows a sudden change of absorbance in the flow cell. The detector uses digital filters to adapt response time to the width of the peaks in your chromatogram.
Page 82: Sample And Reference Wavelength And Bandwidth
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Table 13: Peak Width — Response Time — Data Rate (G7115A/G7165A) Peak width at Response [s] Scan data Scan data Scan data half height rate[Hz] ≤ 251 rate[Hz] ≤ 501 rate[Hz] >...
Page 83 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion The detector can compute and store at run time up to 8 signals with these properties: • sample wavelength, the center of a wavelength band with the width of sample bandwidth (BW), and optionally •...
Page 84 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Anisic acid sample wavelength 252 nm Reference bandwidth 100 nm 30 nm bandwidth Reference wavelength 360 Wavelength (nm) Figure 14: Optimization of Wavelength Setting A wide bandwidth has the advantage of reducing noise by averaging over a wavelength range — compared to a 4 nm bandwidth, the baseline noise is reduced by a factor of approximately 2.5, whereas the signal is about 75 % of a 4 nm wide band.
Page 85: Slit Width
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion Because the detector averages absorbance values that are calculated for each wavelength, using a wide bandwidth does not negatively impact linearity. The use of a reference wavelength is highly recommended to further reduce baseline drift and wander induced by room temperature fluctuations or refractive index changes during a gradient.
Page 86 Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion 16 nm 4 nm 1 nm Figure 17: Benzene at 1, 4 and 16 nm slit width (principle) A wide slit uses more of the light shining through the flow cell. This gives lower baseline noise as shown in Figure 18 on page 86.
Page 87: Optimizing Spectral Acquisition
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion In most cases the width of absorbance bands in the spectrum is more like 30 nm as with anisic acid (Figure 14 on page 84.) In most situations, a slit width of 4 nm will give the best results. Use a narrow slit (1 or 2 nm) if you want to identify compounds with fine spectral structures or if you need to quantify at high concentrations (> 1000 mAU) with a wavelength at the slope of the spectrum.
Page 88: Margin For Negative Absorbance
Optimizing the Performance of the Module Optimizing for Sensitivity, Selectivity, Linearity and Dispersion ag-textnode-version-id: 3358181387 ag-textnode-base-id: 3358181387 Margin for Negative Absorbance The detector adjusts its gain during balance such that the baseline may drift slightly negative (about -100 mAU). In some special case, for example, when gradient with absorbing solvents are used, the baseline may drift to more negative values.
Page 89: Optimizing Selectivity
Optimizing the Performance of the Module Optimizing Selectivity ag-textnode-version-id: 3357539851 ag-textnode-base-id: 3357539851 Optimizing Selectivity ag-textnode-version-id: 3358163851 ag-textnode-base-id: 3358163851 Quantifying Coeluting Peaks by Peak Suppression In chromatography, two compounds may often elute together. A conventional dual-signal detector can only detect and quantify both compounds independently from each other if their spectra do not overlap.
Page 90 Optimizing the Performance of the Module Optimizing Selectivity With a UV-visible detector based on a diode array and the correct choice of a reference wavelength setting, quantitative detection is possible. To suppress caffeine, the reference wavelength must be set to 282 nm. At this wavelength, caffeine shows exactly the same absorbance as at 222 nm.
Page 91: Optimizing The Detector Regarding To The System
The extra-column volume is minimized with the Agilent InfinityLab LC Series system due to the narrow-bore (0.12 mm i.d.) tubing, the low-volume heat exchangers in the column compartment and the flow cell in the detector.
Page 92: How To Achieve Higher Sensitivity
Optimizing the Performance of the Module Optimizing the Detector Regarding to the System ag-textnode-version-id: 3358209035 ag-textnode-base-id: 3358209035 How to Achieve Higher Sensitivity The detector has a number of parameters that are used to optimize performance. The following sections describe how the detector parameters affect performance characteristics: •...
Page 93 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System For example, a signal at wavelength 250 nm with a bandwidth of 16 nm will be an average of the absorbance data from 242 nm to 258 nm. Additionally, a reference wavelength and reference bandwidth can be defined for each signal.
Page 94 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System The reference bandwidth is normally set on a region of the UV spectrum in which the analyte has no absorbance. This is shown in the spectrum for anisic acid in Figure 21 on page 94.
Page 95 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System The detector internally acquires data points faster than is needed for a chromatogram and processes them to produce the signal seen by the data system. Part of the processing reduces the data to an appropriate data rate which allows the chromatographic peaks to be accurately drawn.
Page 96 Optimizing the Performance of the Module Optimizing the Detector Regarding to the System Peak width at Response [s] Scan data Scan data Scan data half height rate[Hz] ≤ 251 rate[Hz] ≤ 501 rate[Hz] > 501 [min] pts/scan pts/scan pts/scan > 0.00625 0.125 >...
Page 97: Warm Up Of The Detector
Optimizing the Performance of the Module Warm up of the Detector ag-textnode-version-id: 3283635595 ag-textnode-base-id: 3283635595 Warm up of the Detector Give the optical unit enough time to warm-up and stabilize (> 60 minutes). The detector is temperature controlled. After turn-on of the detector, it goes through a cycle of different states: •...
Page 98 Optimizing the Performance of the Module Warm up of the Detector Figure 22: Detector Warm-up – 1 hour Figure 23: Detector Warm-up – 2 hour Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 99: Diagnostics And Troubleshooting
ag-textnode-version-id: 18014404593024523 ag-textnode-base-id: 6083542539 Diagnostics and Troubleshooting This chapter gives an overview of the maintenance, troubleshooting, and diagnostic features available. Diagnostic Features 100 User Interfaces 100 Troubleshooting With HPLC Advisor 100 Overview of Available Tests and Tools 101 Tests and Calibrations in Agilent Lab Advisor 101 Maintenance and Troubleshooting Tools of the Module 102 Available Tests vs User Interfaces 102 Introduction 103...
Page 100: Diagnostic Features
Diagnostics and Troubleshooting Diagnostic Features ag-textnode-version-id: 27021601745768715 ag-textnode-base-id: 3981545739 Diagnostic Features This section gives an overview of the diagnostic features available. ag-textnode-version-id: 18014402312290571 ag-textnode-base-id: 3802808587 User Interfaces InfinityLab Assist InfinityLab Assist provides you with assisted troubleshooting and maintenance at your instrument. If the system in use supports the InfinityLab Assist, follow the instructions provided.
Page 101: Overview Of Available Tests And Tools
Diagnostics and Troubleshooting Overview of Available Tests and Tools ag-textnode-version-id: 4060343563 ag-textnode-base-id: 4060343563 Overview of Available Tests and Tools ag-textnode-version-id: 3002950539 ag-textnode-base-id: 3002950539 Tests and Calibrations in Agilent Lab Advisor Use the tests and diagnostic features provided in the Agilent Lab Advisor software to check if your module is working correctly.
Page 102: Maintenance And Troubleshooting Tools Of The Module
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 18014402571216011 ag-textnode-base-id: 4061734027 Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358213515 ag-textnode-base-id: 3358213515 Available Tests vs User Interfaces Depending on the used interface, the available tests and the screens/reports may NOTE vary.
Page 103: Introduction
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 24: Tests in the Agilent Lab Advisor ag-textnode-version-id: 3358814731 ag-textnode-base-id: 3358814731 Introduction All tests are described based on the Agilent Lab Advisor Software B.02.08. Other user interfaces may not provide any test or just a few. For details on the use of the interface refer to the interface documentation.
Page 104 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Table 15: Available Diagnostic Functions vs. Product Level Product Level Tests -ASTM Drift and Noise Test Basic Advanced -Cell Test Basic Advanced -D/A Converter Test Basic Advanced -Dark Current Test Basic Advanced -Filter Test Basic...
Page 105 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Product Level -Vis lamp Basic Advanced -Balance Detector Advanced -UV Lamp Basic Advanced -Method Parameters -Set Signal A Advanced -Set Signal C Advanced -Set Data Rate [HZ] Advanced -Set Signal B Advanced -Module Information -Identify Module...
Page 106: Conditions Of Detector
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358819211 ag-textnode-base-id: 3358819211 Conditions of Detector The test usually should be performed with a detector turned on for at least one hour, so that the temperature regulation of the optical unit is working (not active during the first 30 minutes after turn on).
Page 107: Self-Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358400779 ag-textnode-base-id: 3358400779 Self-Test The self-test runs a series of individual tests (described on the next pages), and evaluates the results automatically. The following tests are run: • Filter Test •...
Page 108 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Self Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 25: Self Test – Results Under the tab Signals you can find the detailed signals from the tests. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 109: Intensity Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358600331 ag-textnode-base-id: 3358600331 Intensity Test The intensity test measures the intensity of the UV-lamp over the full wavelength range (190 - 950 nm). Four spectral ranges are used to evaluate the intensity spectrum.
Page 110: Intensity Test Failed
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Intensity Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 26: Intensity Test – Results (w/o flow cell) Figure 27: Intensity Test – Signals (w/o flow cell) ag-textnode-version-id: 9007202613345035 ag-textnode-base-id: 3358604043 Intensity Test Failed...
Page 111 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Old UV-lamp. • Exchange the UV lamp. Defect optical unit. • If the test fails with new UV-lamp, please contact your Agilent service representative. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 112: Cell Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358676875 ag-textnode-base-id: 3358676875 Cell Test The cell test measures the intensity of the UV- and tungsten lamps over the full wavelength range (190 - 950 nm), once with the flow cell installed, and once with the flow cell removed.
Page 113 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Cell-Test with Agilent Lab Advisor (for further information see Online- Help of user interface). Figure 28: Cell Test – Results Figure 29: Cell Test – Signals (example shows low intensity for flow cell) ag-textnode-version-id: 3358680587 ag-textnode-base-id: 3358680587 Cell Test Failed (low ratio value)
Page 114: Filter Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3447953675 ag-textnode-base-id: 3447953675 Filter Test The filter test checks the correct operation of the filter assembly. When the test is started, the holmium oxide filter is moved into position. During filter movement, the absorbance signal is monitored.
Page 115 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358767755 ag-textnode-base-id: 3358767755 Filter Test Failed Filter Test evaluation Probable cause Suggested actions Filter assembly (lever and • Install the filter assembly. filter) not installed. Defective filter motor. • Please contact your Agilent service representative. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 116 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358793355 ag-textnode-base-id: 3358793355 Holmium Oxide Maximum out of limits Test evaluation Probable cause Suggested actions Holmium oxide filter not • Install the holmium oxide filter. installed. Dirty or contaminated filter. •...
Page 117: Holmium Oxide Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3448529419 ag-textnode-base-id: 3448529419 Holmium Oxide Test The holmium oxide test uses characteristic absorbance maxima of the built-in holmium oxide filter to verify wavelength accuracy (see also Wavelength Verification Test on page 127).
Page 118 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 32: Holmium Oxide Test (Signal) ag-textnode-version-id: 3358411915 ag-textnode-base-id: 3358411915 Holmium Oxide Test Failed Holmium Oxide Test evaluation Probable cause Suggested actions Absorbing solvent or air • Ensure the flow cell is filled with water, and free from air bubble in flow cell.
Page 119 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Dirty or contaminated • Clean optical components with alcohol and lint-free optical components cloth or replace the parts (see ). (achromat, windows). • Clean optical components with alcohol and lint-free cloth or replace the parts (see Intensity Test page 108).
Page 120: Astm Drift And Noise Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358701451 ag-textnode-base-id: 3358701451 ASTM Drift and Noise Test The ASTM noise test determines the detector noise over a period of 20 minutes. The test is done with installed flow cell or flow cell removed. This test also checks for the drift.
Page 121 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the ASTM Drift and Noise Test with Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 33: ASTM Drift and Noise Test – Results (with Flow Cell removed) Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 122 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 34: ASTM Drift and Noise Test – Signal (with Flow Cell removed) ag-textnode-version-id: 3358705163 ag-textnode-base-id: 3358705163 ASTM Noise Test Failed ASTM Noise Test Evaluation Probable cause Suggested actions Insufficient lamp warm-up •...
Page 123 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Dirty or contaminated flow • Flush flow cell cell. • Clean the flow cell as described in Maintenance of Standard, Semi-Micro or Micro Flow Cell on page 194. Old UV-lamp.
Page 124: Slit Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358473483 ag-textnode-base-id: 3358473483 Slit Test The slit test verifies correct operation of the micromechanical slit. During the test, the slit is moved through all slit positions while the detector monitors the lamp intensity change.
Page 125 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Slit Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 35: Slit Test – Results Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 126 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 36: Slit Test – Signal ag-textnode-version-id: 3358477195 ag-textnode-base-id: 3358477195 Slit Test Failed Slit Test Evaluation Probable cause Suggested actions Air bubble in flow cell. • Flush the flow cell or remove the flow cell. Old lamp.
Page 127 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Defective detector main • Please contact your Agilent service representative. board. Defective PDA/optical unit. • Please contact your Agilent service representative. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 128: Wavelength Verification Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358829195 ag-textnode-base-id: 3358829195 Wavelength Verification Test The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the UV-lamp for wavelength calibration. The sharp emission lines enable accurate calibration.
Page 129 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Wavelength Verification Test with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 37: Wavelength Verification – Results Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 130: Wavelength Calibration
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358832907 ag-textnode-base-id: 3358832907 Wavelength Calibration The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the deuterium lamp for wavelength calibration. The sharp emission lines enable more accurate calibration than is possible with holmium oxide. When recalibration is started, the 1 nm slit is moved into the light path automatically.
Page 131 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 1 Run the Wavelength Calibration with the Agilent Lab Advisor (for further information see Online-Help of user interface). Figure 38: Wavelength Calibration - Results If you select No, the test is aborted. If you select Yes, the re-calibration is performed (the offset is corrected).
Page 132 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Probable cause Suggested actions Absorbing solvent or air • Repeat calibration with Flow Cell and compare results. bubble in Flow Cell. Dirty or contaminated Flow • Ensure the flow cell is filled with water, and free from air Cell.
Page 133: D/A Converter (Dac) Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358457099 ag-textnode-base-id: 3358457099 D/A Converter (DAC) Test The detector provides analog output of chromatographic signals for use with integrators, chart recorders or data systems. The analog signal is converted from the digital format by the digital-analog-converter (DAC).
Page 134 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module The noise on the step should be less than 3 µV. Probable cause Suggested actions Bad cable or grounding • Check or replace the cable. problem between detector and external device. Defective detector main •...
Page 135: Dark Current Test
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358536587 ag-textnode-base-id: 3358536587 Dark Current Test The dark-current test measures the leakage current from each diode. The test is used to check for leaking diodes which may cause non-linearity at specific wavelengths.
Page 136: Dark-Current Test Failed
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 3358540299 ag-textnode-base-id: 3358540299 Dark-Current Test Failed Dark-Current Test evaluation Probable cause Suggested actions Defective slit assembly • Run the Self-Test on page 106. (stray light). • Run the Slit Test on page 123 (part of the Self-Test page 106).
Page 137 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module Figure 42: Spectral Scan Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 138: Using The Built-In Test Chromatogram
Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module ag-textnode-version-id: 18014401794165771 ag-textnode-base-id: 3284683787 Using the Built-In Test Chromatogram This function is available from the Agilent ChemStation, Lab Advisor and Instant Pilot. The built-in Test Chromatogram can be used to check the signal path from the detector to the data system and the data analysis or via the analog output to the integrator or data system.
Page 139 Diagnostics and Troubleshooting Maintenance and Troubleshooting Tools of the Module 6 To start a test chromatogram enter in the command line: STRT Figure 43: Test Chromatogram with Agilent Lab Advisor 7 To stop the test chromatogram enter in the command line: STOP The test chromatogram is switched off automatically at the end of a run.
Page 140: Agilent Lab Advisor Software
Lab Advisor Advanced Lab Advisor Basic is included with every Agilent 1200 Infinity Series and Agilent InfinityLab LC Series instrument. The Lab Advisor Advanced features can be unlocked by purchasing a license key, and include real-time monitoring of instrument actuals, all various instrument signals, and state machines.
Page 141: Other Lab Advisor Functions
Diagnostics and Troubleshooting Other Lab Advisor Functions ag-textnode-version-id: 9007203316275083 ag-textnode-base-id: 4061534091 Other Lab Advisor Functions ag-textnode-version-id: 3358892555 ag-textnode-base-id: 3358892555 EMFs - Early Maintenance Feature The EMFs screen allows you to view and manage the EMF counters for all modules in all systems. Figure 44: Early Maintenance Feature Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 142: Error Information
ag-textnode-version-id: 18014404181541003 ag-textnode-base-id: 5672059019 Error Information This chapter describes the meaning of error messages, and provides information on probable causes and suggested actions how to recover from error conditions. What Are Error Messages 144 General Error Messages 145 Timeout 145 Shutdown 145 Remote Timeout 146 Lost CAN Partner 147 Leak 148 Leak Sensor Open 149...
Page 143 Error Information Wavelength Recalibration Lost 168 Heater at Fan Assembly Failed 169 Heater Power at Limit 170 DSP Not Running 171 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 144: What Are Error Messages
Error Information What Are Error Messages ag-textnode-version-id: 18014401511832075 ag-textnode-base-id: 3002350091 What Are Error Messages Error messages are displayed in the user interface when an electronic, mechanical, or hydraulic (flow path) failure occurs that requires attention before the analysis can be continued (for example, repair, or exchange of consumables is necessary).
Page 145: General Error Messages
Error Information General Error Messages ag-textnode-version-id: 72057597564456331 ag-textnode-base-id: 3526528395 General Error Messages General error messages are generic to all Agilent series HPLC modules and may show up on other modules as well. ag-textnode-version-id: 9007202257100299 ag-textnode-base-id: 3002359307 Timeout Error ID: 62 The timeout threshold was exceeded.
Page 146: Shutdown
Error Information General Error Messages ag-textnode-version-id: 27021600766713739 ag-textnode-base-id: 3002490763 Shutdown Error ID: 63 An external instrument has generated a shutdown signal on the remote line. The module continually monitors the remote input connectors for status signals. A LOW signal input on pin 4 of the remote connector generates the error message.
Page 147: Remote Timeout
Error Information General Error Messages ag-textnode-version-id: 9007202257270027 ag-textnode-base-id: 3002529035 Remote Timeout Error ID: 70 A not-ready condition is still present on the remote input. When an analysis is started, the system expects all not-ready conditions (for example, a not-ready condition during detector balance) to switch to run conditions within one minute of starting the analysis.
Page 148: Lost Can Partner
Error Information General Error Messages ag-textnode-version-id: 9007202257121675 ag-textnode-base-id: 3002380683 Lost CAN Partner Error ID: 71 During an analysis, the internal synchronization or communication between one or more of the modules in the system has failed. The system processors continually monitor the system configuration. If one or more of the modules is no longer recognized as being connected to the system, the error message is generated.
Page 149: Leak
Error Information General Error Messages ag-textnode-version-id: 27021600766637707 ag-textnode-base-id: 3002414731 Leak Error ID: 64 A leak was detected in the module. The signals from the two temperature sensors (leak sensor and board-mounted temperature-compensation sensor) are used by the leak algorithm to determine whether a leak is present.
Page 150: Leak Sensor Open
Error Information General Error Messages ag-textnode-version-id: 27021600766781835 ag-textnode-base-id: 3002558859 Leak Sensor Open Error ID: 83 The leak sensor in the module has failed (open circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 151: Leak Sensor Short
Error Information General Error Messages ag-textnode-version-id: 27021600766675979 ag-textnode-base-id: 3002453003 Leak Sensor Short Error ID: 82 The leak sensor in the module has failed (short circuit). The current through the leak sensor is dependent on temperature. A leak is detected when solvent cools the leak sensor, causing the leak sensor current to change within defined limits.
Page 152: Compensation Sensor Open
Error Information General Error Messages ag-textnode-version-id: 54043198531068427 ag-textnode-base-id: 3002622475 Compensation Sensor Open Error ID: 81 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature.
Page 153: Compensation Sensor Short
Error Information General Error Messages ag-textnode-version-id: 54043198531110923 ag-textnode-base-id: 3002664971 Compensation Sensor Short Error ID: 80 The ambient-compensation sensor (NTC) on the power switch board in the module has failed (open circuit). The resistance across the temperature compensation sensor (NTC) on the power switch board is dependent on ambient temperature.
Page 154: Fan Failed
Error Information General Error Messages ag-textnode-version-id: 36028800302691851 ag-textnode-base-id: 3283727883 Fan Failed Error ID: 68 The hall sensor on the fan shaft is used by the mainboard to monitor the fan speed. If the fan speed falls below a certain limit for a certain length of time, the error message is generated.
Page 155: Open Cover
Error Information General Error Messages ag-textnode-version-id: 9007202538630155 ag-textnode-base-id: 3283889163 Open Cover Error ID: 205 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed, the fan is switched off, and the error message is generated. Probable cause Suggested actions The top foam was removed...
Page 156: Cover Violation
Error Information General Error Messages ag-textnode-version-id: 18014401793749899 ag-textnode-base-id: 3284267915 Cover Violation Error ID: 7461 The top foam has been removed. The sensor on the main board detects when the top foam is in place. If the foam is removed while the lamps are on (or if an attempt is made to switch on for example the lamps with the foam removed), the lamps are switched off, and the error message is generated.
Page 157: Eri Messages
Error Information General Error Messages ag-textnode-version-id: 9007202538689547 ag-textnode-base-id: 3283948555 ERI Messages Error ID: 11120, 11121 The ERI (Enhanced Remote Interface) provides two error events related to over current situations on the +5 V and +24 V lines. Probable cause Suggested actions The load on the ERI is too •...
Page 158: Detector Error Messages
Error Information Detector Error Messages ag-textnode-version-id: 9007202612582795 ag-textnode-base-id: 3357841803 Detector Error Messages These errors are detector-specific. ag-textnode-version-id: 3359321355 ag-textnode-base-id: 3359321355 Visible Lamp Current The visible lamp current is missing. The processor continually monitors the lamp current during operation. If the current falls below the lower current limit, the error message is generated.
Page 159: Visible Lamp Voltage
Error Information Detector Error Messages ag-textnode-version-id: 3359653387 ag-textnode-base-id: 3359653387 Visible Lamp Voltage The visible lamp voltage is missing. The processor continually monitors the voltage across the lamp during operation. If the lamp voltage falls below the lower limit, the error message is generated.
Page 160: Diode Current Leakage
Error Information Detector Error Messages ag-textnode-version-id: 9007202614134667 ag-textnode-base-id: 3359393675 Diode Current Leakage Error ID: 1041 When the detector is switched on, the processor checks the leakage current of each of the optical diodes. If the leakage current exceeds the upper limit, the error message is generated.
Page 161: Uv Lamp: No Current
Error Information Detector Error Messages ag-textnode-version-id: 9007202538702475 ag-textnode-base-id: 3283961483 UV Lamp: No Current Error ID: 7450 The lamp anode current is missing. The processor continually monitors the anode current drawn by the lamp during operation. If the anode current falls below the lower current limit, the error message is generated.
Page 162: Uv Lamp: No Voltage
Error Information Detector Error Messages ag-textnode-version-id: 9007202538757643 ag-textnode-base-id: 3284016651 UV Lamp: No Voltage Error ID: 7451 The lamp anode voltage is missing. The processor continually monitors the anode voltage across the lamp during operation. If the anode voltage falls below the lower limit, the error message is generated.
Page 163: Lamp Ignition Failed
Error Information Detector Error Messages ag-textnode-version-id: 9007202538774795 ag-textnode-base-id: 3284033803 Lamp Ignition Failed Error ID: 7452 The lamp failed to ignite. The processor monitors the lamp current during the ignition cycle. If the lamp current does not rise above the lower limit within 2 –...
Page 164: No Heater Current
Error Information Detector Error Messages ag-textnode-version-id: 9007202538796171 ag-textnode-base-id: 3284055179 No Heater Current Error ID: 7453 The lamp heater current in the detector is missing. During lamp ignition, the processor monitors the heater current. If the current does not rise above the lower limit within 1 , the error message is generated.
Page 165: Calibration Values Invalid
Error Information Detector Error Messages ag-textnode-version-id: 9007202614292235 ag-textnode-base-id: 3359551243 Calibration Values Invalid Error ID: 1036 The calibration values read from the spectrometer ROM are invalid. After recalibration, the calibration values are stored in ROM. The processor periodically checks if the calibration data are valid. If the data are invalid or cannot be read from the spectrometer ROM, the error message is generated.
Page 166: Holmium Oxide Test Failed
Error Information Detector Error Messages ag-textnode-version-id: 3359572619 ag-textnode-base-id: 3359572619 Holmium Oxide Test Failed Probable cause Suggested actions Lamps switched off. • Ensure the lamps are switched on. Defective or dirty flow cell. • Ensure the flow cell is inserted correctly, and is free from contamination (cell windows, buffers etc.).
Page 167: Illegal Temperature Value From Sensor On Main Board
Error Information Detector Error Messages ag-textnode-version-id: 9007202539118987 ag-textnode-base-id: 3284377995 Illegal Temperature Value from Sensor on Main Board Error ID: 1071 This temperature sensor (located on the detector main board) delivered a value outside the allowed range. The parameter of this event equals the measured temperature in 1/100 centigrade.
Page 168: Illegal Temperature Value From Sensor At Air Inlet
Error Information Detector Error Messages ag-textnode-version-id: 9007202539089163 ag-textnode-base-id: 3284348171 Illegal Temperature Value from Sensor at Air Inlet Error ID: 1072 This temperature sensor delivered a value outside the allowed range. The parameter of this event equals the measured temperature in 1/100 centigrade. As a result the temperature control is switched off.
Page 169: Wavelength Recalibration Lost
Error Information Detector Error Messages ag-textnode-version-id: 9007202614368779 ag-textnode-base-id: 3359627787 Wavelength Recalibration Lost Error ID: 1037 The calibration information needed for your detector to operate correctly has been lost. During calibration of the detector the calibration values are stored in ROM. If no data is available in the spectrometer ROM, the error message is generated.
Page 170: Heater At Fan Assembly Failed
Error Information Detector Error Messages ag-textnode-version-id: 9007202538979083 ag-textnode-base-id: 3284238091 Heater at Fan Assembly Failed Error ID: 1073 Every time the deuterium lamp or the tungsten lamp (DAD only) is switched on or off a heater self-test is performed. If the test fails an error event is created. As a result the temperature control is switched off.
Page 171: Heater Power At Limit
Error Information Detector Error Messages ag-textnode-version-id: 9007202538936331 ag-textnode-base-id: 3284195339 Heater Power at Limit Error ID: 1074 The available power of the heater reached either the upper or lower limit. This event is sent only once per run. The parameter determines which limit has been hit: 0 means upper power limit hit (excessive ambient temperature drop).
Page 172: Dsp Not Running
Error Information Detector Error Messages ag-textnode-version-id: 9007202614096395 ag-textnode-base-id: 3359355403 DSP Not Running Error ID: 1034 This error message comes up when the communication between the optical unit and the main board has a problem. Probable cause Suggested actions Random communication •...
Page 173: Maintenance
ag-textnode-version-id: 27021601842317323 ag-textnode-base-id: 4078094347 Maintenance This chapter provides general information on maintenance of the module. Introduction to Maintenance 174 Safety Information Related to Maintenance 175 Overview of Maintenance 177 Cleaning the Module 178 Remove and Install Doors 179 Replace a Lamp 182 Remove and Install a Flow Cell 188 Maintenance of Standard, Semi-Micro or Micro Flow Cell 194 Maintenance of High Pressure Flow Cell 199 Replacing Capillaries on a Standard Flow Cell 201...
Page 174: Introduction To Maintenance
Maintenance Introduction to Maintenance ag-textnode-version-id: 3284748811 ag-textnode-base-id: 3284748811 Introduction to Maintenance The module is designed for easy maintenance. Maintenance can be done from the front with module in place in the system. There are no serviceable parts inside. NOTE Do not open the module. ag-struc-frag-safety-version-id: 3369125003 ag-struc-frag-safety-base-id: 3369125003 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 175: Safety Information Related To Maintenance
Maintenance Safety Information Related to Maintenance ag-textnode-version-id: 18014402382780683 ag-textnode-base-id: 3873298699 Safety Information Related to Maintenance Eye damage by detector light WARNING ag-struc-frag-safety-version-id: 9007202540437131 ag-struc-frag-safety-base-id: 3285696139 Eye damage may result from directly viewing the UV-light produced by the lamp of the optical system used in this product. —...
Page 176 Maintenance Safety Information Related to Maintenance Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3003809803 ag-struc-frag-safety-base-id: 3003809803 The handling of solvents, samples and reagents can hold health and safety risks. — When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 177: Overview Of Maintenance
Maintenance Overview of Maintenance ag-textnode-version-id: 3358901515 ag-textnode-base-id: 3358901515 Overview of Maintenance The following pages describe maintenance (simple repairs) of the detector that can be carried out without opening the main cover. Table 16: Overview of Maintenance Procedure Typical Frequency Notes Cleaning of module If required.
Page 178: Cleaning The Module
Maintenance Cleaning the Module ag-textnode-version-id: 3002023307 ag-textnode-base-id: 3002023307 Cleaning the Module To keep the module case clean, use a soft cloth slightly dampened with water, or a solution of water and mild detergent. Avoid using organic solvents for cleaning purposes. They can cause damage to plastic parts. Liquid dripping into the electronic compartment of your module can cause WARNING shock hazard and damage the module...
Page 179: Remove And Install Doors
Maintenance Remove and Install Doors ag-textnode-version-id: 18014402225732235 ag-textnode-base-id: 3716250251 Remove and Install Doors When • The instrument doors or the hinges are broken. Tools required Qty. Description 5023-3138 Reversible Screwdriver + Blade 1,0 x 5,5 Parts required Qty. Description (Infinity III) 5004-3140 Door Kit Infinity III 140mm Parts required...
Page 180 Maintenance Remove and Install Doors 1 Press the release buttons and pull the front door out. 2 For the Installation of the front door, insert the hinges into their guides and push the door in until the release buttons click into their final position. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 181 Maintenance Remove and Install Doors Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 182: Replace A Lamp
Maintenance Replace a Lamp ag-textnode-version-id: 9007202613650699 ag-textnode-base-id: 3358909707 Replace a Lamp When • If noise or drift exceeds application limits or lamp does not ignite Tools required Qty. Description Screwdriver, Pozidriv #1 PT3 Parts required Qty. Description 2140-0820 Longlife Deuterium lamp “C“ (with black cover and RFID tag) G1103-60001 Tungsten lamp...
Page 183 Maintenance Replace a Lamp 1 Open the doors. 2 Grab the lamp cover and pull it off (it is fixed by two magnets in the center of the cover). 3 Unplug the lamp connector as required. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 184 Maintenance Replace a Lamp 4 Unscrew (do not remove) the two lamp screws (Pozidriv) as required. Injury by touching hot lamp WARNING If the detector has been in use, the lamp may be hot. — If so, wait for lamp to cool down. 5 Remove the deuterium lamp and place it on a clean place.
Page 185 Maintenance Replace a Lamp 6 Remove the Vis-lamp and place it on a clean place. Do not touch the glass bulb with your fingers. It may reduce the light output. NOTE 7 Insert the deuterium lamp (RFID tag on the top side). 8 Insert the Vis-lamp (flat side to the right).
Page 186 Maintenance Replace a Lamp 9 Fasten the lamp screws. 10 Reconnect the lamp connector as required. 11 Place the lamp cables in the lamp cover. 12 Make sure that the cables are in the cover. Slide the lamp cover into the top position of the metal front and press the lamp cover completely in until it clicks.
Page 187 Maintenance Replace a Lamp 13 Close the doors. 14 Perform a Wavelength Verification Test on page 127 or a Holmium Oxide Test on page 117 to check the correct positioning of the lamp. 15 Perform an Intensity Test. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 188: Remove And Install A Flow Cell
Maintenance Remove and Install a Flow Cell ag-textnode-version-id: 9007202613721995 ag-textnode-base-id: 3358981003 Remove and Install a Flow Cell For bio-inert modules use bio-inert parts only! When • If an application needs a different type of flow cell or the flow cell needs repair.
Page 189 Maintenance Remove and Install a Flow Cell 1 Open the doors. 2 Grab the lamp cover and pull it off (it is fixed by two magnets in the center of the cover). 3 Open the flow cell door. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 190 Maintenance Remove and Install a Flow Cell 4 Disconnect the flow cell inlet capillary (top) from the union. 5 Disconnect the waste tubing (bottom) from the union. 6 Loosen the thumb screw (1.) and remove the flow cell outlet capillary (bottom) with the union (2.).
Page 191 Maintenance Remove and Install a Flow Cell 7 Remove the flow cell while pressing the flow cell holder. The label attached to the flow cell provides information on part number, path NOTE length, and maximum pressure. If you want to replace flow cell parts, see Maintenance of Standard, Semi-Micro or Micro Flow Cell on page 194 or...
Page 192 Maintenance Remove and Install a Flow Cell 9 Insert the flow cell capillaries into the union holder (top is inlet, bottom is outlet). 10 Tighten the thumb screw. 11 Reconnect the waste tubing (bottom) to the union. Establish a flow and check for leaks.
Page 193 Maintenance Remove and Install a Flow Cell 12 Close the flow cell door. 13 Make sure that the cables are in the cover. Slide the lamp cover into the top position of the metal front and press the lamp cover completely in until it clicks.
Page 194: Maintenance Of Standard, Semi-Micro Or Micro Flow Cell
Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell ag-textnode-version-id: 3359006987 ag-textnode-base-id: 3359006987 Maintenance of Standard, Semi-Micro or Micro Flow Cell When • If the flow cell needs repair due to leaks or contaminations (reduced light throughput) Tools required Qty. Description Wrench, 1/4 inch for capillary connections...
Page 195 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell 1 Use a 4 mm hex key to unscrew the window assembly (1) and remove the gasket (6,7) from the cell body. Carefully take one of the gaskets (#6 back or # 7 front) and insert it into the cell NOTE body.
Page 196 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell 4 Assemble the washers and the window assembly in correct order. 5 Correct orientation of spring washers (2) is required. 1 - Window screw 2 - Spring washers 3 - Compression washer 4 - Window holder 5 - Quartz window 6 - Gasket...
Page 197 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell 6 Press the PTFE ring into the window assembly. 7 Press the window assembly onto the new or cleaned quartz window. 8 Insert the window assembly (1) into the cell body. Do not mix the gasket #6 and #7 (different hole diameter).
Page 198 Maintenance Maintenance of Standard, Semi-Micro or Micro Flow Cell 10 Reconnect the capillaries, see Remove and Install a Flow Cell on page 188. 11 Perform a leak test. 12 Insert the flow cell. 13 Replace the front cover 14 Perform a Wavelength Verification Test on page 127 or a Holmium Oxide...
Page 199: Maintenance Of High Pressure Flow Cell
Maintenance Maintenance of High Pressure Flow Cell ag-textnode-version-id: 3358946827 ag-textnode-base-id: 3358946827 Maintenance of High Pressure Flow Cell When • If the flow cell needs repair due to leaks or contaminations (reduced light throughput) Tools required Qty. Description Wrench, 1/4 inch for capillary connections, or 5043-0915 Fitting mounting tool Hexagonal key, 4 mm...
Page 200 Maintenance Maintenance of High Pressure Flow Cell If you want to replace the gasket only, reinsert the window assembly into the cell body. 2 Use a tooth pick to remove the quartz window from the window assembly. If the washers fall out of the window assembly, they must be inserted in the NOTE correct order with the PTFE ring to prevent any leaks from the flow cell window.
Page 201: Replacing Capillaries On A Standard Flow Cell
Maintenance Replacing Capillaries on a Standard Flow Cell ag-textnode-version-id: 3358984715 ag-textnode-base-id: 3358984715 Replacing Capillaries on a Standard Flow Cell For bio-inert modules use bio-inert parts only! When • If the capillary is blocked Tools required Qty. Description Wrench, 1/4 inch 5043-0915 Fitting mounting tool Wrench, 4 mm Screwdriver, Pozidriv #1 PT3...
Page 202 Maintenance Replacing Capillaries on a Standard Flow Cell Sample degradation and contamination of the instrument CAUTION ag-struc-frag-safety-version-id: 3360735243-3 ag-struc-frag-safety-base-id: 3360735243 Metal parts in the flow path can interact with the bio-molecules in the sample leading to sample degradation and contamination. —...
Page 203 Maintenance Replacing Capillaries on a Standard Flow Cell 3 To replace the inlet capillary, use a 4 mm wrench to open the fitting. 4 Unscrew the cell body from the heat exchanger. 5 Remove the heat exchanger from the clamp unit. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 204 Maintenance Replacing Capillaries on a Standard Flow Cell 6 Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation. See Note at the beginning of this section. 7 Unscrew the fixing screw and unwrap the inlet capillary from the grove in the flow cell body.
Page 205 Maintenance Replacing Capillaries on a Standard Flow Cell 9 Bend the capillary again by 90° as shown below. 90° 10 Insert the capillary into the hole between fixing screw and the inlet fitting. 11 The capillary lays in the grove and should be tied around the body (in the grove) 5 times.
Page 206 Maintenance Replacing Capillaries on a Standard Flow Cell 12 Insert the fixing screw, so that the capillary cannot leave the grove. 13 Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section. 14 Fix the heat exchanger to the clamp unit.
Page 207 Maintenance Replacing Capillaries on a Standard Flow Cell 15 Fix the flow cell body to the heat exchanger. 16 Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench. 17 After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
Page 208 Maintenance Replacing Capillaries on a Standard Flow Cell 18 Check for a centered holder vs. hole. If required adjust with the holder screws. 19 Reconnect the capillaries, see Remove and Install a Flow Cell on page 188. 20 Perform a leak test. 21 Insert the flow cell.
Page 209: Replacing Capillaries On A Semi-Micro And Micro Flow Cell
Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell ag-textnode-version-id: 3358950539 ag-textnode-base-id: 3358950539 Replacing Capillaries on a Semi-Micro and Micro Flow Cell When • If the capillary is blocked Tools required Qty. Description Wrench, 1/4 inch for capillary connections, or 5043-0915 Fitting mounting tool Wrench, 4 mm...
Page 210 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell 1 Identify the inlet and outlet capillaries. Inlet Outlet 2 Remove the outlet capillary. 3 To replace the inlet capillary, use a 4 mm wrench to open the fitting. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 211 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell 4 Unscrew the cell body from the heat exchanger. 5 Remove the heat exchanger from the clamp unit. 6 Use a small flat screw driver to carefully lift off the I.D. tag. Shown is the default orientation.
Page 212 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell 7 Carefully insert the I.D. tag into the new heat exchanger. Shown is the default orientation. See Note at the beginning of this section. 8 Fix the heat exchanger to the clamp unit. 9 Fix the flow cell body to the heat exchanger.
Page 213 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell 10 Fix the inlet capillary to the flow cell body handtight first. Then do a 1/4 turn with a 4 mm wrench. 11 After replacing the outlet capillary, fix it handtight first. Then do a 1/4 turn with a 4 mm wrench.
Page 214 Maintenance Replacing Capillaries on a Semi-Micro and Micro Flow Cell 15 Insert the flow cell. 16 Close the doors. 17 Perform a Wavelength Verification Test on page 127 or a Holmium Oxide Test on page 117 to check the correct positioning of the lamp. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 215: Nano Flow Cell - Replacing Or Cleaning
Maintenance Nano Flow Cell - Replacing or Cleaning ag-textnode-version-id: 3358954251 ag-textnode-base-id: 3358954251 Nano Flow Cell - Replacing or Cleaning When • If parts are contaminated or leaky. Tools required Qty. Description Screwdriver, Pozidriv #1 PT3 Wrench, 1/4 inch for capillary connections Parts required Qty.
Page 216 Maintenance Nano Flow Cell - Replacing or Cleaning 2 Unscrew the cell body from the holder. 3 Unscrew the capillaries from the flow cell. DO NOT use the adapter at this time! Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 217 Maintenance Nano Flow Cell - Replacing or Cleaning 4 Using for example a toothpick, press on the plastic part and slide the quartz body out of the cell housing. 5 The quartz body and the cell seal assembly can be separated for cleaning purpose.
Page 218 Maintenance Nano Flow Cell - Replacing or Cleaning 6 This figure shows the correct holding of the quartz body and the cell seal assembly. 7 Replace the cell seal assembly onto the quartz body. Always use a new seal assembly to exclude damage during disassembling. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 219 Maintenance Nano Flow Cell - Replacing or Cleaning 8 Slide the quartz body completely into the cell body to the front stop (use for example a toothpick). 9 Insert the flow cell capillaries and tighten them fingertight. Use the wrench and torque adapter as described on Figure 46 on page 221 and tighten the...
Page 220: Wrench Plus Torque
Maintenance Nano Flow Cell - Replacing or Cleaning 10 Reassemble the flow cell body to the holder. 11 Install the flow cell, see Remove and Install a Flow Cell on page 188. 12 Perform a leak test with the flow cell outside of the detector. 13 If no leak is observed, install the flow cell and you are ready to work.
Page 221 Maintenance Nano Flow Cell - Replacing or Cleaning Adapter Wrench DO NOT press down more than shown here 0.7 Nm Figure 46: Wrench plus Torque Adapter Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 222: Cleaning Or Exchanging The Holmium Oxide Filter
Maintenance Cleaning or Exchanging the Holmium Oxide Filter ag-textnode-version-id: 9007202613654411 ag-textnode-base-id: 3358913419 Cleaning or Exchanging the Holmium Oxide Filter When • If holmium oxide filter is contaminated Tools required Qty. Description Screwdriver, Pozidriv #1 PT3 Screwdriver, flat blade Wrench, 1/4 inch for capillary connections Pair of tweezers Parts required Qty.
Page 223 Maintenance Cleaning or Exchanging the Holmium Oxide Filter 2 If not already in this position, move the filter up. 3 Release the holder with a screw driver (at the top). Do not scratch the holmium oxide filter. NOTE 4 Remove the holmium oxide filter. The holmium oxide filter can be cleaned with alcohol and a lint-free cloth.
Page 224 Maintenance Cleaning or Exchanging the Holmium Oxide Filter 5 Insert the holmium oxide filter. 6 Replace the flow cell cover and fix the six screws. 7 Perform a holmium oxide test (see Holmium Oxide Test on page 117) to check the proper function of the holmium oxide filter. 8 Install the flow cell, see Remove and Install a Flow Cell on page 188.
Page 225: Correcting Leaks
Maintenance Correcting Leaks ag-textnode-version-id: 9007202613729419 ag-textnode-base-id: 3358988427 Correcting Leaks When • If a leakage has occurred in the flow cell area or at the heat exchanger or at the capillary connections Tools required Qty. Description Tissue Wrench, 1/4 inch for capillary connections 5043-0915 Fitting mounting tool Use tissue to dry the leak sensor area and the leak pan.
Page 226 Maintenance Correcting Leaks 3 Open the flow cell door. 4 Observe the capillary connections and the flow cell area for leaks and correct, if required. To check for leaks, establish a flow and observe the flow cell (outside of the cell NOTE compartment) and all capillary connections.
Page 227 Maintenance Correcting Leaks 6 Install the flow cell and close the flow cell door. 7 Make sure that the cables are in the cover. Slide the lamp cover into the top position of the metal front and press the lamp cover completely in until it clicks.
Page 228: Replace Leak Handling System Parts
Maintenance Replace Leak Handling System Parts ag-textnode-version-id: 9007202613703435 ag-textnode-base-id: 3358962443 Replace Leak Handling System Parts When • If the parts are corroded or broken Parts required Qty. Description 5043-0856 Leak Adapter 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/ 9 mm 1 Open the doors. 2 Press the Leak Adapter down (1.) and remove it together with the tubing (2.).
Page 229 Maintenance Replace Leak Handling System Parts 3 Install the Leak Adapter by pressing it into the Main Cover. 4 Insert the Tubing (approximately 85 mm required for replacement) between Leak Adapter outlet and Leak Panel. 5 Close the doors. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 230: Replace The Module Firmware
Maintenance Replace the Module Firmware ag-textnode-version-id: 18014401511543691 ag-textnode-base-id: 3002061707 Replace the Module Firmware When Install a newer firmware • It fixes known problems of older versions, or • It introduces new features, or • It ensures keeping all systems at the same (validated) revision When Install an older firmware •...
Page 231: Module Specific Information
Maintenance Replace the Module Firmware Module Specific Information Table 17: Module specific Information (G7115A DAD/G7165A MWD) G7115A DAD G7165A MWD Initial firmware (main and D.07.01 D.07.01 resident) Compatible with When using the G7115A or G7165A in a system, all other modules 1260/1290 Infinity modules must have firmware revision from firmware set 7.00 or above (main and resident).
Page 232: Information From Module's Assemblies
Maintenance Information from Module’s Assemblies ag-textnode-version-id: 3357788043 ag-textnode-base-id: 3357788043 Information from Module’s Assemblies Lamp and Flow Cell RFID Tag The detector is equipped with a UV lamp and flow cell identification system using RFID (radio frequency identification) tags attached to the assemblies and RFID tag readers at the optical unit.
Page 233: Parts And Materials For Maintenance
ag-textnode-version-id: 27021601842609803 ag-textnode-base-id: 4078386827 Parts and Materials for Maintenance This chapter provides information on parts for maintenance. Overview of Maintenance Parts 234 Standard Flow Cell 236 Standard Flow Cell Bio-inert 238 Semi-Micro Flow Cell 240 Micro Flow Cell 242 High Pressure Flow Cell 244 Prep Flow Cell - SST 246 Prep Flow Cell - Quartz 248 Nano Flow Cells 250 Accessory Kits 256...
Page 234: Overview Of Maintenance Parts
Parts and Materials for Maintenance Overview of Maintenance Parts ag-textnode-version-id: 9007202613887755 ag-textnode-base-id: 3359146763 Overview of Maintenance Parts Figure 47: Maintenance Parts Qty. Description 5360-0016 Door 140mm left Infinity III (only orderable as part of 5004-3140 Door Kit Infinity III 140mm) 5360-0015 Door 140mm right Infinity III (only orderable as part of 5004-3140...
Page 235 Parts and Materials for Maintenance Overview of Maintenance Parts Qty. Description 5043-0856 Leak Adapter 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/9 mm for Waste and Leak Adapter (ca. 85 mm required) Flow cell with RFID tag for details refer to specific flow cell G1315-60022 Standard flow cell, 10 mm, 13 µL, 120 bar (12 MPa) G5615-60022...
Page 236: Standard Flow Cell
Parts and Materials for Maintenance Standard Flow Cell ag-textnode-version-id: 3359160203 ag-textnode-base-id: 3359160203 Standard Flow Cell Figure 48: Standard Flow Cell Parts Description G1315-60022 Standard flow cell, 10 mm, 13 µL, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553 Washer kit (10/pk) 79883-28801 Compression washer 79883-22301 Window holder 1000-0488 Quartz window...
Page 237 Parts and Materials for Maintenance Standard Flow Cell Description G1315-87302 Capillary OUT (0.17 mm, 200 mm lg) G1315-84910 Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp 5022-2184 Union, stand LC flow, no fitting G1315-68712 Cell repair kit STD includes window screw kit, 4 mm hexagonal wrench and seal kit 79883-68703 Window screw kit, includes 2 quartz windows,...
Page 238: Standard Flow Cell Bio-Inert
Parts and Materials for Maintenance Standard Flow Cell Bio-inert ag-textnode-version-id: 9007202613869835 ag-textnode-base-id: 3359128843 Standard Flow Cell Bio-inert Figure 50: Standard Flow Cell Bio-inert Description G5615-60022 Standard flow cell bio-inert, 10 mm, 13 µL, 120 bar (12 MPa) for MWD/DAD, includes 0890-1763 – 0.18 x 1500 mm PEEK capillary and 5063-6591 – PEEK fittings 79883-22402 Window screw 5062-8553...
Page 239 Parts and Materials for Maintenance Standard Flow Cell Bio-inert Description G5615-87331 Capillary In (0.17 mm, 590 mm lg), including heat exchanger) G5615-87302 Capillary Out (0.17 mm, 200 mm lg) G1315-84910 Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp 5022-2184 Union, stand LC flow, no fitting G1315-68712 Cell repair kit STD includes window screw kit, 4 mm hexagonal wrench and...
Page 240: Semi-Micro Flow Cell
Parts and Materials for Maintenance Semi-Micro Flow Cell ag-textnode-version-id: 3359133323 ag-textnode-base-id: 3359133323 Semi-Micro Flow Cell Figure 52: Semi-Micro Flow Cell Parts Description G1315-60025 Semi-micro flow cell, 6 mm, 5 µL, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553 Washer kit (10/pk) 79883-28801 Compression washer 79883-22301 Window holder 1000-0488 Quartz window...
Page 241 Parts and Materials for Maintenance Semi-Micro Flow Cell Description G1315-87306 Capillary OUT (0.12 mm, 200 mm lg) G1315-87302 Capillary OUT (0.17 mm, 200 mm lg) G1315-84910 Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp 5022-2184 Union, stand LC flow, no fitting G1315-68713 Cell repair kit semi-micro, includes window screw kit, Gasket Kit BACK, Gasket Kit FRONT and 4 mm hexagonal...
Page 242: Micro Flow Cell
Parts and Materials for Maintenance Micro Flow Cell ag-textnode-version-id: 3359137803 ag-textnode-base-id: 3359137803 Micro Flow Cell Figure 54: Micro Flow Cell Parts Description G1315-60024 Micro flow cell, 3 mm, 2 µL, 120 bar (12 MPa) 79883-22402 Window screw 5062-8553 Washer kit (10/pk) 79883-28801 Compression washer 79883-22301 Window holder 1000-0488 Quartz window...
Page 243 Parts and Materials for Maintenance Micro Flow Cell Description G1315-87302 Capillary OUT (0.17 mm, 200 mm lg) G1315-84910 Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp 5022-2184 Union, stand LC flow, no fitting G1315-68713 Cell repair kit semi-micro, includes window screw kit, Gasket Kit BACK, Gasket Kit FRONT and 4 mm hexagonal wrench 79883-68703...
Page 244: High Pressure Flow Cell
Parts and Materials for Maintenance High Pressure Flow Cell ag-textnode-version-id: 3359115403 ag-textnode-base-id: 3359115403 High Pressure Flow Cell Figure 56: High pressure flow cell - parts Description G1315-60015 High pressure flow cell, 6 mm, 1.7 µL, 400 bar (40 MPa) Window assembly, comprises items 2, 3, 4, 5 and 6 79883-27101 Seal ring 1000-0953...
Page 245 Parts and Materials for Maintenance High Pressure Flow Cell Description G1315-87312 Capillary ST 0.12 mm x 150 mm S/S G1315-87311 Capillary ST 0.17 mm x 380 mm S/S 79883-68700 High pressure cell repair kit (includes 1 quartz window, 1 compression washer, 5 spring washers, 2 seal rings) Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 246: Prep Flow Cell - Sst
Parts and Materials for Maintenance Prep Flow Cell - SST ag-textnode-version-id: 3359151243 ag-textnode-base-id: 3359151243 Prep Flow Cell - SST For more details on the Preparative Flow Cells refer to the technical note that NOTE comes with the flow cells. ag-struc-frag-safety-version-id: 3552914443 ag-struc-frag-safety-base-id: 3552914443 - inlet 4 - outlet...
Page 247 Parts and Materials for Maintenance Prep Flow Cell - SST Description G1315-27706 Cell body G1315-84901 Clamp unit G1315-84902 Handle for Clamp unit 0515-1056 Screw M 2.5, 4 mm lg for cell body/clamp Gaskets #2 and #3 have different hole diameters. NOTE 1 - Window screw 2 - Spring washers 3 - Compression washer 4 - Window holder...
Page 248: Prep Flow Cell - Quartz
Parts and Materials for Maintenance Prep Flow Cell - Quartz ag-textnode-version-id: 3359110923 ag-textnode-base-id: 3359110923 Prep Flow Cell - Quartz For more details on the Preparative Flow Cells refer to the technical note that NOTE comes with the flow cells. Figure 59: Prep Flow Cell - Quartz Parts Description G1315-60017 Prep flow cell quartz, 0.3 mm, 20 bar (2 MPa)
Page 249 Parts and Materials for Maintenance Prep Flow Cell - Quartz Description G1315-80003 Quartz body - Prep Cell 0.06 mm G1315-67302 PTFE tubing 80 cm length, 0.5 mm i.d., o.D. 1.6 mm G1315-27705 Cell housing G1315-67301 PTFE tubing 2 m length, 0.8 mm i.d., o.D. 1.6 mm 0100-1516 Finger-tight fitting PEEK, 2/pk The flow cell comes with two tubings 0.8 mm i.d.
Page 250: Nano Flow Cells
Parts and Materials for Maintenance Nano Flow Cells ag-textnode-version-id: 3359119883 ag-textnode-base-id: 3359119883 Nano Flow Cells The following kits are available: Table 19: Nano-flow cell kits Part number Comments G1315-68724 completely assembled (includes items 1, 2, 3, 4, 10, 11, 12, 13, 14, 15, and 16) completely assembled (includes items 1, 2, 3, 4, 10, 11, 12, 13, 14, 15, and 16) Figure 60...
Page 251 Parts and Materials for Maintenance Nano Flow Cells Generic parts for both nano-flow cells: 3, 4 Figure 60: Content of kits Description 5063-6593 Fitting Screw (for 4 mm wrench) Cell ferrules are factory installed 5065-4422 PEEK fitting 1/32" 5063-6592 Litetouch ferrules LT-100, (1/32" Ferrule and SS lock ring) 5022-2146 Union Adjustment Tool 5022-2184...
Page 252 Parts and Materials for Maintenance Nano Flow Cells Specific parts for the semi-nano flow cell 3, 4 Figure 61: Content of kits Description G1315-68724 500 nl Flow cell kit, 10 mm, 500 nL, 5 MPa G1315-87333 PEEK coated fused silica capillary Inlet (100 µm) pre- mounted to cell, includes Inlet capillary, 300 mm long, 100 µm i.d.
Page 253 Parts and Materials for Maintenance Nano Flow Cells Description G1315-68715 Sealing Kit Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 254 Parts and Materials for Maintenance Nano Flow Cells Specific parts for the nano flow cell 3, 4 Figure 62: Content of kits Description G1315-87323 PEEK coated fused silica capillary Inlet (50 µm) alternative, includes Inlet capillary, 400 mm long, 50 µm i.d. with pre-fixed ferrules (#4) and fittings (#3), plus one PEEK Fitting FT (#5) G1315-87328 PEEK coated fused silica capillary Outlet (50 µm),...
Page 255 Parts and Materials for Maintenance Nano Flow Cells Description G1315-68725 Sealing Kit 80 nL cell Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 256: Accessory Kits
Parts and Materials for Maintenance Accessory Kits ag-textnode-version-id: 3358374923 ag-textnode-base-id: 3358374923 Accessory Kits G7115-68755 (Detector Accessory Kit) contains the following items: Description 5062-8535 Waste accessory kit (Flow Cell to waste) 5500-1155 Tube Connector, 90 degree, ID 6.4 0100-1516 Finger-tight fitting PEEK, 2/pk 5181-1516 CAN cable, Agilent module to module, 0.5 m 5500-1191...
Page 257: Holmium Oxide Filter
Parts and Materials for Maintenance Holmium Oxide Filter ag-textnode-version-id: 3359088523 ag-textnode-base-id: 3359088523 Holmium Oxide Filter Figure 63: Holmium Oxide Filter Parts Description G7115-68700 Filter motor assembly (includes filter lever G1315-45001 and spring 1460-1510) 1460-1510 Spring G1315-45001 Filter lever 79880-22711 Holmium oxide filter When the filter motor has been removed, the filter lever should not be reused.
Page 258: Leak Handling Parts
Parts and Materials for Maintenance Leak Handling Parts ag-textnode-version-id: 9007202613838475 ag-textnode-base-id: 3359097483 Leak Handling Parts Figure 64: Leak Parts Description 5043-0856 Leak Adapter 5063-6527 Tubing, Silicon Rubber, 1.2 m, ID/OD 6 mm/9 mm 5061-3356 Leak Sensor Assembly G7115-45000 Leak Plane for Infinity II & III DAD WR 0515-2529 Screw Tapping PAN-HD-TORX T10 3x8 ST-ZN (not shown)
Page 259: Identifying Cables
ag-textnode-version-id: 27021601743836299 ag-textnode-base-id: 3979613323 Identifying Cables This chapter provides information on cables used with the modules. Cable Overview 260 Analog Cables 262 Remote Cables 264 BCD Cables 268 CAN/LAN Cables 270 RS-232 Cables 271 USB 272 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 260: Cable Overview
Identifying Cables Cable Overview ag-textnode-version-id: 9007202256869899 ag-textnode-base-id: 3002128907 Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations. Analog cables Description 35900-60750 Agilent 35900A A/D converter...
Page 261 Identifying Cables Cable Overview RS-232 cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 262: Analog Cables
Identifying Cables Analog Cables ag-textnode-version-id: 9007202256873995 ag-textnode-base-id: 3002133003 Analog Cables One end of these cables provides a BNC connector to be connected to Agilent modules. The other end depends on the instrument to which connection is being made. Agilent Module to 35900 A/D converters p/n 35900-60750 35900 Pin Agilent...
Page 263 Identifying Cables Analog Cables Agilent Module to General Purpose p/n 01046-60105 Pin Agilent Signal Name module Not connected Black Analog - Analog + Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 264: Remote Cables
Identifying Cables Remote Cables ag-textnode-version-id: 9007202256882571 ag-textnode-base-id: 3002141579 Remote Cables ERI (Enhanced Remote Interface) • 5188-8029 ERI to general purpose (D-Sub 15 pin male - open end) • 5188-8044 ERI to ERI (D_Sub 15 pin male - male) • 5188-8059 ERI-Extension-Cable 1.2 m (D-Sub15 pin male / female) p/n 5188-8029 Color code Enhanced Remote Classic Remote...
Page 265 Identifying Cables Remote Cables • 5188-8045 ERI to APG (Connector D_Subminiature 15 pin (ERI), Connector D_Subminiature 9 pin (APG)) p/n 5188-8045 Pin (ERI) Signal Pin (APG) Active (TTL) Start Request Stop Ready High Power on High Future Shut Down Start Prepare Ground Cable Shielding Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 266 Ground Cable Shielding One end of these cables provides an Agilent Technologies APG (Analytical Products Group) remote connector to be connected to Agilent modules. The other end depends on the instrument to be connected to. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 267 Identifying Cables Remote Cables Agilent Module to Agilent 35900 A/D Converters p/n 5061-3378 Pin 35900 A/D Pin Agilent Signal Name Active module (TTL) 1 - White 1 - White Digital ground 2 - Brown 2 - Brown Prepare run 3 - Gray 3 - Gray Start 4 - Blue 4 - Blue Shut down 5 - Pink...
Page 268: Bcd Cables
Identifying Cables BCD Cables ag-textnode-version-id: 3358242315 ag-textnode-base-id: 3358242315 BCD Cables One end of these cables provides a 15-pin BCD connector to be connected to the Agilent modules. The other end depends on the instrument to be connected to Agilent Module to General Purpose p/n G1351-81600 Wire Color Pin Agilent...
Page 269 Identifying Cables BCD Cables Agilent Module to 3396 Integrators p/n 03396-60560 Pin 3396 Pin Agilent Signal Name BCD Digit module BCD 5 BCD 7 BCD 6 BCD 4 BCD0 BCD 3 BCD 2 BCD 1 Digital ground + 5 V Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 270: Can/Lan Cables
Identifying Cables CAN/LAN Cables ag-textnode-version-id: 18014401511606795 ag-textnode-base-id: 3002124811 CAN/LAN Cables Both ends of this cable provide a modular plug to be connected to Agilent modules CAN or LAN connectors. Can Cables Description 5181-1516 CAN cable, Agilent module to module, 0.5 m 5181-1519 CAN cable, Agilent module to module, 1 m LAN Cables Description...
Page 271: Rs-232 Cables
Identifying Cables RS-232 Cables ag-textnode-version-id: 9007202256878091 ag-textnode-base-id: 3002137099 RS-232 Cables Description RS232-61601 RS-232 cable, 2.5 m Instrument to PC, 9-to-9 pin (female). This cable has special pin-out, and is not compatible with connecting printers and plotters. It is also called "Null Modem Cable" with full handshaking where the wiring is made between pins 1-1, 2-3, 3-2, 4-6, 5-5, 6-4, 7-8, 8-7, 9-9.
Page 272: Usb
Identifying Cables ag-textnode-version-id: 9007202256886667 ag-textnode-base-id: 3002145675 To connect a USB Flash Drive use a USB OTG cable with Mini-B plug and A socket. Description 5188-8050 USB A M-USB Mini B 3 m (PC-Module) 5188-8049 USB A F-USB Mini B M OTG (Module to Flash Drive) Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 273: Hardware Information
ag-textnode-version-id: 18014401867282187 ag-textnode-base-id: 3357800203 Hardware Information This chapter describes the module in more detail on hardware and electronics. General Hardware Information 274 Firmware Description 274 Electrical Connections 276 Interfaces 278 Instrument Layout 285 Early Maintenance Feedback (EMF) 286 Module-Specific Hardware Information 288 Setting the 6-bit Configuration Switch 288 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 274: General Hardware Information
Hardware Information General Hardware Information ag-textnode-version-id: 36028801083399691 ag-textnode-base-id: 4064435723 General Hardware Information This section provides detailed hardware information on firmware that is valid for this module. ag-textnode-version-id: 3002159627 ag-textnode-base-id: 3002159627 Firmware Description The firmware of the instrument consists of two independent sections: •...
Page 275: Firmware Updates
Hardware Information General Hardware Information • or module specific functions like • internal events such as lamp control, filter movements, • raw data collection and conversion to absorbance. Firmware Updates Firmware updates can be done with the Agilent Lab Advisor software with files on the hard disk (latest version should be used).
Page 276: Electrical Connections
• The ERI connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as start, stop, common shut down, prepare, and so on. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 277: Rear View Of The Module
— Any other instruments connected to this instrument shall be approved to a suitable safety standard and must include reinforced insulation from the mains. Never use cables other than the ones supplied by Agilent Technologies to ensure NOTE proper functionality and compliance with safety or EMC regulations.
Page 278: Serial Number Information
(there can be more than one code for the same module) 00000 Serial number ag-textnode-version-id: 18014402069660043 ag-textnode-base-id: 3560178059 Interfaces The Agilent InfinityLab LC Series modules provide the following interfaces: Table 21: Agilent InfinityLab LC Series interfaces Module RS-232 Analog APG (A) / Special...
Page 279 Hardware Information General Hardware Information Module RS-232 Analog APG (A) / Special (on-board) ERI (E) G7157A Detectors G7114A/B G7115A G7117A/B/C G7121A/B G7162A/B G7165A Fraction Collectors G7158B G7159B G7166A Requires a host module with on-board LAN with minimum FW B.06.40 or C.06.40, or with additional G1369C LAN Card G1364E/F, G5664B...
Page 280: Overview Interfaces
Hardware Information General Hardware Information LAN connection is made between at least one of the Agilent modules and the NOTE Control PC. — If an Assist Hub is installed, connect the LAN to the Lab LAN port of this module. —...
Page 281 Remote (ERI) The ERI (Enhanced Remote Interface) connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features as common shut down, prepare, and so on. It allows easy connection between single instruments or systems to ensure coordinated analysis with simple coupling requirements.
Page 282: Special Interfaces
Hardware Information General Hardware Information for next analysis, followed by START of run and optional STOP of run triggered on the respective lines. In addition PREPARE and START REQUEST may be issued. The signal levels are defined as: • standard TTL levels (0 V is logic true, + 5.0 V is false), •...
Page 283 ERI replaces the AGP Remote Interface that is used in the HP 1090/1040/1050/1100 HPLC systems and Agilent 1100/1200/1200 Infinity HPLC modules. All new InfinityLab LC Series products using the communication board core electronics use ERI. This interface is already used in the Agilent Universal Interface Box 2 (UIB2)
Page 284 Hardware Information General Hardware Information Enhanced Remote PGND +24 V ERI out +24 V ERI out IO (Input/Output) Lines • Eight generic bi-directional channels (input or output). • Same as the APG Remote. • Devices like valves, relays, ADCs, DACs, controllers can be supported/ controlled.
Page 285: Instrument Layout
Hardware Information General Hardware Information • If a connected device requires more power the firmware detects this (overcurrent detection) and provides the information to the user interface. • Fuse used for safety protection (on board). • Short circuit will be detected through hardware. ag-textnode-version-id: 3002198795 ag-textnode-base-id: 3002198795 USB (Universal Serial Bus)
Page 286: Early Maintenance Feedback (Emf)
Hardware Information General Hardware Information ag-textnode-version-id: 3002155147 ag-textnode-base-id: 3002155147 Early Maintenance Feedback (EMF) Maintenance requires the exchange of components that are subject to wear or stress. Ideally, the frequency at which components are exchanged should be based on the intensity of use of the module and the analytical conditions, and not on a predefined time interval.
Page 287 Hardware Information General Hardware Information displayed values) as EMF limits, and then reset the EMF counters to zero. The next time the EMF counters exceed the new EMF limits, the EMF flag will be displayed, providing a reminder that maintenance needs to be scheduled. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 288: Module-Specific Hardware Information
Hardware Information Module-Specific Hardware Information ag-textnode-version-id: 18014402588076171 ag-textnode-base-id: 4078594187 Module-Specific Hardware Information ag-textnode-version-id: 18014402036253963 ag-textnode-base-id: 3526771979 Setting the 6-bit Configuration Switch The 6-bit configuration switch is located at the rear of the module with communication board electronics. Switch settings provide configuration parameters for LAN and instrument specific initialization procedures.
Page 289: Special Settings
Hardware Information Module-Specific Hardware Information Mode Init Mode Test Boot Main System/Revert to Default Data Test Boot Resident System/Revert to Default Data Legend: 0 (switch down), 1 (switch up), SW (switch) ag-textnode-version-id: 3285054731 ag-textnode-base-id: 3285054731 Special Settings Boot-Resident/Main Firmware update procedures may require this mode in case of firmware loading errors (main/resident firmware part).
Page 290 Hardware Information Module-Specific Hardware Information Loss of data CAUTION ag-struc-frag-safety-version-id: 3182477195 ag-struc-frag-safety-base-id: 3182477195 Forced cold start erases all methods and data stored in the non-volatile memory. Exceptions are calibration settings, diagnosis and repair log books which will not be erased. —...
Page 291: Lan Configuration
ag-textnode-version-id: 27021601047391371 ag-textnode-base-id: 3283168395 LAN Configuration This chapter provides information on connecting the module to the control software. What You Have to Do First 292 TCP/IP Parameter Configuration 293 Configuration Switch 294 Initialization Mode Selection 295 Dynamic Host Configuration Protocol (DHCP) 297 General Information (DHCP) 297 Setup (DHCP) 297 Manual Configuration 300 With Telnet 300...
Page 292: What You Have To Do First
LAN Configuration What You Have to Do First ag-textnode-version-id: 3285075339 ag-textnode-base-id: 3285075339 What You Have to Do First The module has an on-board LAN communication interface. This chapter is generic and may show figures that differ from your module. The NOTE functionality is the same.
Page 293: Tcp/Ip Parameter Configuration
LAN Configuration TCP/IP Parameter Configuration ag-textnode-version-id: 3285094667 ag-textnode-base-id: 3285094667 TCP/IP Parameter Configuration To operate properly in a network environment, the LAN interface must be configured with valid TCP/IP network parameters. These parameters are: • IP address • Subnet Mask • Default Gateway The TCP/IP parameters can be configured by the following methods: •...
Page 294: Configuration Switch
LAN Configuration Configuration Switch ag-textnode-version-id: 3285079051 ag-textnode-base-id: 3285079051 Configuration Switch The configuration switch can be accessed at the rear of the module. Configuration switch Figure 71: Location of configuration switch The module is shipped with all switches set to OFF, as shown above. To perform any LAN configuration, SW1 and SW2 must be set to OFF.
Page 295: Initialization Mode Selection
LAN Configuration Initialization Mode Selection ag-textnode-version-id: 3285107339 ag-textnode-base-id: 3285107339 Initialization Mode Selection The following initialization (init) modes are selectable: Table 24: Initialization mode switches Init Mode Use Default IP Address Use Stored IP Address Use DHCP Note: The setting ‘0’ (down) is essential. Legend: 0 (switch down), 1 (switch up), SW (switch) Default IP address for LAN is 192.168.254.11.
Page 296: Using Default
LAN Configuration Initialization Mode Selection Using Default When Using Default is selected, the factory default parameters are taken instead. These parameters enable a TCP/IP connection to the LAN interface without further configuration, see Table 25 on page 296. Active Default Parameter Parameter Figure 73: Using Default (principle) Using the default address in your local area network may result in network...
Page 297: Dynamic Host Configuration Protocol (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) ag-textnode-version-id: 3560226315 ag-textnode-base-id: 3560226315 Dynamic Host Configuration Protocol (DHCP) ag-textnode-version-id: 3285099147 ag-textnode-base-id: 3285099147 General Information (DHCP) The Dynamic Host Configuration Protocol (DHCP) is an auto configuration protocol used on IP networks. The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware.
Page 298: Setup (Dhcp)
LAN Configuration Dynamic Host Configuration Protocol (DHCP) ag-textnode-version-id: 3285103627 ag-textnode-base-id: 3285103627 Setup (DHCP) The DHCP functionality is available on all Agilent HPLC modules with on-board LAN Interface or LAN Interface Card G1369C, and “B”-firmware (B.06.40 or above) or modules with "D"-firmware. All modules should use latest firmware from the same set.
Page 299 LAN Configuration Dynamic Host Configuration Protocol (DHCP) Table 27: LC Modules with 8-bit configuration switch (B-firmware) (configuration switch at rear of the instrument) SW 6 SW 7 SW 8 Initialization Mode DHCP 3 Turn on the module that hosts the LAN interface. 4 Configure your Control Software (e.g.
Page 300: Manual Configuration
LAN Configuration Manual Configuration ag-textnode-version-id: 9007202781250955 ag-textnode-base-id: 3526509963 Manual Configuration Manual configuration only alters the set of parameters stored in the non-volatile memory of the module. It never affects the currently active parameters. Therefore, manual configuration can be done at any time. A power cycle is mandatory to make the stored parameters become the active parameters, given that the initialization mode selection switches are allowing it.
Page 301: With Telnet
LAN Configuration Manual Configuration ag-textnode-version-id: 3285087243 ag-textnode-base-id: 3285087243 With Telnet Whenever a TCP/IP connection to the module is possible (TCP/IP parameters set by any method), the parameters may be altered by opening a Telnet session. 1 Open the system (DOS) prompt window by clicking on Windows START button and select “Run...”.
Page 302 LAN Configuration Manual Configuration Table 28: Telnet commands Value Description displays syntax and descriptions of commands displays current LAN settings ip <x.x.x.x> sets new ip address sm <x.x.x.x> sets new subnet mask gw <x.x.x.x> sets new default gateway exit exits shell and saves all changes 4 To change a parameter follows the style: •...
Page 303 LAN Configuration Manual Configuration change of IP setting to Initialization mode is Using Stored active TCP/IP settings stored TCP/IP settings in non-volatile memory connected to PC with controller software (e.g. Agilent ChemStation), here not Telnet - Change IP settings connected 7 When you have finished typing the configuration parameters, type exit press Enter to exit with storing parameters.
Page 304: With The Instant Pilot (G4208A)
LAN Configuration Manual Configuration ag-textnode-version-id: 3285090955 ag-textnode-base-id: 3285090955 With the Instant Pilot (G4208A) To configure the TCP/IP parameters before connecting the module to the network, the Instant Pilot (G4208A) can be used. 1 From the Welcome screen press the More button. 2 Select Configure.
Page 305: Pc And User Interface Software Setup
LAN Configuration PC and User Interface Software Setup ag-textnode-version-id: 3595223819 ag-textnode-base-id: 3595223819 PC and User Interface Software Setup ag-textnode-version-id: 3596853899 ag-textnode-base-id: 3596853899 PC Setup for Local Configuration This procedure describes the change of the TCP/IP settings on your PC to match the module’s default parameters in a local configuration (see Table 25 page 296).
Page 306 LAN Configuration PC and User Interface Software Setup 2 You may enter here the fixed IP address of the module or use the Alternative Configuration. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 307 LAN Configuration PC and User Interface Software Setup 3 We will use the direct LAN access via Cross-over LAN cable with the module’s IP address. 4 Click on OK to save the configuration. Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 308: Appendix
Flow Cell 324 At-a-Glance Details About Agilent Capillaries 325 Waste Electrical and Electronic Equipment (WEEE) Directive 329 Radio Interference 330 RFID Statement 331 Sound Emission 333 UV-Radiation 334 Declaration of Conformity for HOX2 Filter 335 Agilent Technologies on Internet 337 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 309: General Safety Information
Appendix General Safety Information ag-textnode-version-id: 63050397785402635 ag-textnode-base-id: 3002215691 General Safety Information The following general safety precautions must be observed during all phases of operation, service, and repair of this instrument. Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design, manufacture, and intended use of the instrument.
Page 310: Before Applying Power
— Verify that the voltage range and frequency of your power distribution matches to the power specification of the individual instrument. — Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations.
Page 311: Do Not Operate In An Explosive Atmosphere
Appendix General Safety Information ag-textnode-version-id: 3002235275 ag-textnode-base-id: 3002235275 Do Not Operate in an Explosive Atmosphere Presence of flammable gases or fumes WARNING ag-struc-frag-safety-version-id: 3004420107 ag-struc-frag-safety-base-id: 3004420107 Explosion hazard — Do not operate the instrument in the presence of flammable gases or fumes.
Page 312: Solvent Information
Appendix General Safety Information ag-textnode-version-id: 18014402035996427 ag-textnode-base-id: 3526514443 Solvent Information Toxic, flammable and hazardous solvents, samples and reagents WARNING ag-struc-frag-safety-version-id: 3004430475-2 ag-struc-frag-safety-base-id: 3004430475 The handling of solvents, samples and reagents can hold health and safety risks. — When working with these substances observe appropriate safety procedures (for example by wearing goggles, safety gloves and protective clothing) as described in the material handling and safety data sheet supplied by the vendor, and follow good laboratory practice.
Page 313 Appendix General Safety Information Recommendations on the Use of Solvents Observe the following recommendations on the use of solvents. • Brown glass ware can avoid growth of algae. • Follow the recommendations for avoiding the growth of algae, see the pump manuals.
Page 314: Refrigerant
Appendix General Safety Information ag-textnode-version-id: 3285065867 ag-textnode-base-id: 3285065867 Flow cell To protect optimal functionality of your flow-cell: • Avoid the use of alkaline solutions (pH > 9.5) which can attack quartz and thus impair the optical properties of the flow cell. ag-textnode-version-id: 18014401794544139 ag-textnode-base-id: 3285062155 Refrigerant Table 29: Physical properties of refrigerant R600a (isobutane)
Page 315: Magnets
Appendix General Safety Information ag-textnode-version-id: 27021600810554507 ag-textnode-base-id: 3046331531 Magnets Figure 81: Magnets in doors of pumps, autosamplers, detectors, and fraction collectors ag-textnode-version-id: 27021600766477323 ag-textnode-base-id: 3002254347 Safety Symbols Table 30: Symbols The apparatus is marked with this symbol when the user shall refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage.
Page 316 Appendix General Safety Information Manufacturing date. Product Number Serial Number Power symbol indicates On/Off. The apparatus is not completely disconnected from the mains supply when the on/off switch is in the Off position Pacemaker Magnets could affect the functioning of pacemakers and implanted heart defibrillators. A pacemaker could switch into test mode and cause illness.
Page 317: Material Information
3358303627 Materials Used in the Bio-inert LC System For the Bio-inert LC system, Agilent Technologies uses highest-quality materials in the flow path (also referred to as wetted parts), which are widely accepted by life science scientists, as they are known for optimum inertness to biological samples and ensure best compatibility with common samples and solvents over a wide pH range.
Page 318 Appendix Material Information Table 31: Used bio-inert materials Module Materials Agilent 1260 Infinity III Bio-inert Pump Titanium, gold, platinum-iridium, ceramic, (G5654A) ruby, PTFE, PEEK Agilent 1260 Infinity III Bio-inert Multisampler Upstream of sample introduction: (G5668A) • Titanium, gold, PTFE, PEEK, ceramic Downstream of sample introduction: •...
Page 319: General Information About Solvent/Material Compatibility
Appendix Material Information ag-textnode-version-id: 3002994315 ag-textnode-base-id: 3002994315 General Information About Solvent/Material Compatibility Materials in the flow path are carefully selected based on Agilent’s experiences in developing highest-quality instruments for HPLC analysis over several decades. These materials exhibit excellent robustness under typical HPLC conditions. For any special condition, please consult the material information section or contact Agilent.
Page 320 Appendix Material Information Polyphenylene Sulfide (PPS) Polyphenylene sulfide has outstanding stability even at elevated temperatures. It is resistant to dilute solutions of most inorganic acids, but it can be attacked by some organic compounds and oxidizing reagents. Nonoxidizing inorganic acids, such as sulfuric acid and phosphoric acid, have little effect on polyphenylene sulfide, but at high concentrations and temperatures, they can still cause material damage.
Page 321 Appendix Material Information Polyimide Agilent uses semi-crystalline polyimide for rotor seals in valves and needle seats in autosamplers. One supplier of polyimide is DuPont, which brands polyimide as Vespel, which is also used by Agilent. Polyimide is stable in a pH range between 1 and 10 and in most organic solvents. It is incompatible with concentrated mineral acids (e.g.
Page 322 Appendix Material Information • Halogenated solvents or mixtures, which form radicals and/or acids, for example: 2 CHCl → 2 COCl + 2 HCl This reaction, in which stainless steel probably acts as a catalyst, occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol.
Page 323 Appendix Material Information Fused Silica and Quartz (SiO Fused silica is used in Max Light Cartridges. Quartz is used for classical flow cell windows. It is inert against all common solvents and acids except hydrofluoric acid and acidic solvents containing fluorides. It is corroded by strong bases and should not be used above pH 12 at room temperature.
Page 324: Flow Cell
Appendix Material Information The tubing of the leak sensor is made of PVDF (polyvinylidene fluoride), which is incompatible with the solvent DMF (dimethylformamide). Sapphire, Ruby, and Al -Based Ceramics Sapphire, ruby, and ceramics based on aluminum oxide Al are inert to almost all common acids, bases, and solvents.
Page 325: At-A-Glance Details About Agilent Capillaries
Appendix At-a-Glance Details About Agilent Capillaries ag-textnode-version-id: 18014401512496395 ag-textnode-base-id: 3003014411 At-a-Glance Details About Agilent Capillaries The following section provides useful information about Agilent capillaries and its characteristics. Syntax for capillary description Type - Material - Capillary dimensions - Fitting Left/Fitting right Table 32: Example for a capillary description Code provided Meaing of the code...
Page 326 Appendix At-a-Glance Details About Agilent Capillaries Color Coding Guide Table 33: Color-coding key for Agilent capillary tubing Internal diameter in mm Color code 0.015 Orange 0.025 Yellow 0.05 Beige 0.075 Black 0.075 MP35N Black with orange stripe 0.1 Purple 0.12 0.12 MP35N Red with orange stripe 0.17 ...
Page 327 Appendix At-a-Glance Details About Agilent Capillaries Description Tube Tubing Heat exchanger Heat exchanger Abbreviation Guide for Material Table 35: Material (indicates which raw material is used for the capillary) Description Stainless steel Titanium PEEK FS/PK PEEK-coated fused silica PK/ST Stainless steel-coated PEEK PFFE PTFE Fused silica...
Page 328 Appendix At-a-Glance Details About Agilent Capillaries Abbreviation Guide for Fitting Left/Fitting Right Table 37: Fitting left/fitting right (indicates which fitting is used on both ends of the capillary) Description Swagelok + 0.8 mm Port id Swagelok + 1.6 mm Port id Metric M4 + 0.8 mm Port id Metric M3 + 1.6 mm Port id Swagelok union Long...
Page 329: Waste Electrical And Electronic Equipment (Weee) Directive
Appendix Waste Electrical and Electronic Equipment (WEEE) Directive ag-textnode-version-id: 3002258059 ag-textnode-base-id: 3002258059 Waste Electrical and Electronic Equipment (WEEE) Directive This product complies with the European WEEE Directive marking requirements. The affixed label indicates that you must not discard this electrical/electronic product in domestic household waste.
Page 330: Radio Interference
Radio Interference ag-textnode-version-id: 3002261771 ag-textnode-base-id: 3002261771 Radio Interference Cables supplied by Agilent Technologies are screened to provide optimized protection against radio interference. All cables are in compliance with safety or EMC regulations. Test and Measurement If test and measurement equipment is operated with unscreened cables, or used for measurements on open set-ups, the user has to assure that under operating conditions the radio interference limits are still met within the premises.
Page 331: Rfid Statement
Appendix RFID Statement ag-textnode-version-id: 18014403738255755 ag-textnode-base-id: 5228773771 RFID Statement Brasil Este equipamento não tem direito à proteção contra interferência prejudicial e não pode causar interferência em sistemas devidamente autorizados. Para mais informações, consulte o site da Anatel: https://www.gov.br/anatel/pt-br. Este produto não é apropriado para uso em ambientes domésticos, pois poderá causar interferências eletromagnéticas que obrigam o usuário a tomar medidas necessárias para minimizar estas interferências.
Page 332 Appendix RFID Statement Thailand เครื � อ งโทรคมนาคมและอุ ป กรณ์ น ี � ม ี ค วามสอดคล้ อ งตามมาตรฐานหรื อ ข้ อ กํ า หนดทางเทคนิ ค ของ กสทช. This telecommuinication equipment conforms to NTC/NBTC technical requirement. 1. User Information according to FCC 15.21:Changes or modifications not expressly approved by the party responsible for compliance could void the user’s authority to operate the equipment.
Page 333: Sound Emission
Appendix Sound Emission ag-textnode-version-id: 3002271243 ag-textnode-base-id: 3002271243 Sound Emission Sound Pressure Sound pressure Lp < 70 db(A) according to DIN EN ISO 7779 Schalldruckpegel Schalldruckpegel Lp < 70 db(A) nach DIN EN ISO 7779 Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 334: Uv-Radiation
Appendix UV-Radiation ag-textnode-version-id: 3358258699 ag-textnode-base-id: 3358258699 UV-Radiation This information is only valid for UV-lamps without cover (e.g. 2140-0590 and NOTE 2140-0813). Emissions of ultraviolet radiation (200-315 nm) from this product is limited such that radiant exposure incident upon the unprotected skin or eye of operator or service personnel is limited to the following TLVs (Threshold Limit Values) according to the American Conference of Governmental Industrial Hygienists: Table 39: UV-Radiation Limits...
Page 335: Declaration Of Conformity For Hox2 Filter
Appendix Declaration of Conformity for HOX2 Filter ag-textnode-version-id: 3285070859 ag-textnode-base-id: 3285070859 Declaration of Conformity for HOX2 Filter Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 336 Appendix Declaration of Conformity for HOX2 Filter Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 337: Agilent Technologies On Internet
Appendix Agilent Technologies on Internet ag-textnode-version-id: 3002266251 ag-textnode-base-id: 3002266251 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at: https://www.agilent.com Diode Array Detector WR and Multiple Wavelength Detector User Manual...
Page 338 • introduction and specifications, • installation, • using and optimizing, • troubleshooting and diagnose, • maintenance, • parts identification, • hardware information, • safety and related information. www.agilent.com © Agilent Technologies Inc. 2016-2024 Edition: 10/2024 Document No: SD-29000218 Rev. F...

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