Instructions For Kingfisher Flex With 96 Deep Well Plates For Dna Purification From 400 Μl Of Plant Lysate - Thermo Scientific KingFisher Plant DNA Kit Instruction Manual

Instructions for
KingFisher Flex
with 96 deep well
plates for DNA
purification from
400 µl of plant lysate
Thermo Fisher Scientific
These instructions are for the DNA purification from
400 µl of plant lysate using the KingFisher Plant DNA Kit
(Cat. No. 97050196, 1 x 96) and the KingFisher Flex with
96 deep well plates.
1. Homogenize the samples according to the instructions
given in "Homogenization of sample material"
page 22.
2. Take five empty Microtiter deep well 96 plates and two
empty Thermo Scientific KingFisher Flex 96 KF plates.
3. Prepare five Microtiter deep well 96 plates and one
KingFisher Flex 96 KF plate as indicated in the table
below. Resuspend the KingFisher Magnetic Beads well
(e.g., by vortexing) before transferring them from the
bottle.
Plate Plate
number type
1 Microtiter
deep well
96 plate
2 Microtiter
deep well
96 plate
3 Microtiter
deep well
96 plate
4 Microtiter
deep well
96 plate
5 Microtiter
deep well
96 plate
6 KingFisher
Flex 96
KF plate
Thermo Scientific KingFisher Plant DNA Kit Instruction Manual
Protocols and Pipetting Instructions
Homogenization of sample material
Plate Content
name
Sample Plant lysate
KingFisher
Magnetic Beads
Binding Buffer
Wash 1 Wash Buffer 1
Wash 2 Wash Buffer 2
Wash 80% ethanol
EtOH
Wash 3 Wash Buffer 3
Elution Elution Buffer
on
Sample/reagent
volume per well
400 µl
30 µl
400 µl
600 µl
600 µl
600 µl
600 µl
150 µl
23