Procedures After The Run - GE AKTA GO Operating Instructions Manual

6.6

Procedures after the run

Introduction
After the run, the instrument and column should be cleaned to prevent bacterial growth,
sample contamination in the next run, and column clogging.
This section describes how to clean the column using a Column CIP (Cleaning-In-Place)
method, prepare the column and the instrument for storage, and how to shut down the
system.
Create a CIP method
To create a Column CIP method, use the following procedure.
Step
1
2
3
4
5
Run a column CIP
To clean the column using the Column CIP method, use the following procedure.
Step
1
2
3
4
ÄKTA go Operating Instructions 29360951 AC
Action
Open the Method Editor module and click the Create new method button.
Select Column CIP method.
In the method settings, select the column being used.
In Column CIP phase, click get suggested steps.
Note:
The suggested steps are only available for some techniques and might not
be optimized for your column. Read the instructions provided with the column
to get the recommended CIP steps.
Click the save button, choose the system, name, and location for your method
and click Save.
Action
Open the System Control module and click the Open Method Navigator
button.
Select the Column CIP method created above and click the Run button.
Make sure that the inlet tubings are in the correct solutions.
Click Start.
6 Run a method

6.6 Procedures after the run

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