After Run Procedures - GE ÄKTA pure Operating Instructions Manual

6.5

After run procedures

Introduction
This section describes how to clean the instrument and columns after a chromatographic
run, and how to prepare the system for storage.
The instrument and the columns should be cleaned between the runs. This will prevent,
for example, sample contamination, protein precipitation and column clogging. If the
instrument is not going to be used for a couple of days or longer, the instrument, columns
and the pH flow cell should be filled with storage solution. For further information about
cleaning and maintenance procedures, see ÄKTA pure System Handbook.
Tip:
System cleaning
After a method run is completed, perform the following:
•
•
•
•
•
ÄKTA pure Operating Instructions 29-0229-97 AE
To clean and fill the instrument and columns with storage solution, use System
CIP and Column CIP either as separate, predefined methods or as phases in-
cluded in a chromatographic method.
CAUTION
Hazardous chemicals and biological agents. Before maintenance,
service and decommissioning, wash the ÄKTA pure instrument with
a neutral solution to make sure that any hazardous solvents and
biological agents have been flushed out from the system.
Rinse the instrument with one or several cleaning solution(s) (e.g., NaOH, buffer solu-
tion or distilled water) using System CIP.
Note:
If Column valve V9-C or V9H-C is mounted, the integrated pressure sensor
of the valve allows the system to monitor the post-column pressure. The
limit for the pressure sensor in Column valve V9-C or V9H-C is automati-
cally set so that the UV monitor and the pH monitor are protected from
high pressure. If Column valve V9-C or V9H-C is not mounted, make sure
to keep the pressure in the system after the column below the pressure
limits for the modules in the flow path.
If applicable, empty the fraction collector.
Clean all spills on the instrument and on the bench using a moist tissue.
Empty the waste vessel.
Clean the manual injection port of the injection valve.
6 Run a method

6.5 After run procedures

119