Create A Method - GE AKTA GO Operating Instructions Manual

6 Run a method

6.1 Create a method

6.1

Create a method

Create a new method using the
Method Editor
Follow the steps below to create a method using a predefined method template. The
example given uses an Affinity Chromatography method from UNICORN 7.4. The Method
Settings phase sets up parameters that are used throughout the method, such as unit
for method base and flow rate.
There are more ways to create methods in UNICORN. Refer to the UNICORN Method
Manual for more information.
Step
1
2
3
4
80
Action
Open the Method Editor module, click the New Method button and choose
the system and a suitable predefined method.
Open the Phase Properties tab and confirm that all of the selections made
in the phases correspond to your intended method by following the steps
below.
In the Method Settings phase, select column type to get suitable values, or
enter correct values at Column volume (column volume), Pressure limit
(pressure limit) and Flow rate (flow rate).
In the Equilibration phase, set a suitable volume and concentration of buffer
B.
ÄKTA go Operating Instructions 29360951 AC