Molecular Devices ImageXpress Pico Installation Manual page 53

Items Provided by You
You must provide the following items for the environmental control system:
Required Gas Supplies: The following gas supplies are required for all experiments using
environmental control:
Optional Gas Supply: The following gas supply is required only for hypoxia experiments:
Pressure regulators and Fittings: Delivers gases from the cylinders to the instrument. Gas
pressure to the instrument must be regulated within the range of 0.8 bar to 1.2 bar (11.6 psi
to 17.4 psi). The ideal setting is 1.0 bar (14.5 psi). See
Regulator Details on page 73
Gas Supply Tubing: Molecular Devices provides 10 m (32.8 ft) of tubing to connect the
instrument to the regulator. If this is not sufficient, you must provide an appropriate length
of 4 mm I.D. / 6 mm O.D. polyurethane tubing.
Teflon Tape and Hose Clamps: Secures tubing and regulator fittings.
Ultrapure Water (18 Mohm•cm): Maintains humidity inside the environmental control
cassette. The humidifying column can hold up to 130 ml (4.4 oz) of ultrapure water and must
be refilled before the level drops to 50 ml (1.7 oz).
5063666 K
Compressed Air Source: Supplies pressurized air from a cylinder, a lab gas line, or an
oil-free air pump or compressor.
Pressurized CO Source: Supplies pressurized CO
2
least 99% pure.
Note: CO is used to regulate the pH of cell culture media for mammalian cells.
2
If you are using an organic buffer solution (for example, HEPES) to regulate the
pH of your media, then a CO
Pressurized N Source: Supplies pressurized N
2
99% pure. Required for hypoxia experiments; otherwise optional.
Chapter 5: Environmental Control System
source may not be required.
2
from a cylinder. Gas must be at least
2
Environmental Control System Gas
for details.
from a cylinder. Gas must be at
2
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