Microplate handling system for the spectramax paradigm multi-mode detection platform (110 pages)
Summary of Contents for Molecular Devices ImageXpress Pico
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ImageXpress Pico Automated Cell Imaging System with CellReporterXpress Software Version 2.7 Installation Guide 5063666 K October 2020...
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For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of Molecular Devices, LLC or their respective owners. These trademarks may not be used in any type of promotion or advertising without the prior written permission of Molecular Devices, LLC.
Contents Safety Information Warnings, Cautions, Notes, and Tips Symbols on Instrument Labels Protective Housing Electrical Safety Moving Parts Safety Lifting Hazard Chemical and Biological Safety Chapter 1: ImageXpress Pico Automated Cell Imaging System ImageXpress Pico System Features CellReporterXpress Software Features Theory of Operation Obtaining Support Product Documentation About This Guide...
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ImageXpress Pico Automated Cell Imaging System Installation Guide Chapter 5: Environmental Control System Environmental Control System Warnings and Cautions Environmental Control System Hardware Using the Environmental Control System Maintaining the Environmental Control System Troubleshooting the Environmental Control System Appendix A: Instrument Specifications...
When warnings and cautions are displayed in this guide, be careful to follow the specific safety information related to them. The following user-attention statements can be displayed in the text of Molecular Devices user documentation. Each statement implies a particular amount of observation or recommended...
ImageXpress Pico Automated Cell Imaging System Installation Guide Symbols on Instrument Labels Each safety label found on the instrument contains an alert symbol that indicates the type of potential safety hazard related to the label. The following table lists the alert symbols that can be found on Molecular Devices instruments.
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For products under the requirement of the WEEE directive, contact your dealer or local Molecular Devices office for the procedures to facilitate the proper collection, treatment, recovery, recycling, and safe disposal of the device. Indicates the environmental friendly use period for China RoHS. The symbol may indicate the number of years in the use period.
Electrical Safety To prevent electrically related injuries and property damage, inspect all electrical equipment before use and immediately report all electrical deficiencies. Contact Molecular Devices Technical Support to service equipment that requires the removal of covers or panels. WARNING! HIGH VOLTAGE.
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Safety Information Replacing Fuses If the instrument does not power on when you press the Power button, confirm that the power cord is securely connected to a functioning power outlet and to the power port on the rear of the instrument. If the power fails while the instrument was running, verify that the power cord is not loose or disconnected and that power to the power outlet is functioning properly.
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When the status light on the Power button is amber, the instrument is fully powered off. Note: If the ImageXpress Pico system doesn't respond, you can perform a hard shutdown by holding the Power button for five seconds.
Lifting Hazard WARNING! LIFTING HAZARD. The ImageXpress Pico system weighs approximately 38 kg (84 lb). Use great care when lifting or moving the instrument. To prevent injury, use a minimum of two people to lift the instrument.
ImageXpress Pico Automated Cell Imaging System Installation Guide Chemical and Biological Safety Normal operation of the instrument can involve the use of materials that are toxic, flammable, or otherwise biologically harmful. When using such materials, observe the following precautions: Handle infectious samples based on good laboratory procedures and methods to prevent the spread of disease.
When used in combination with the CellReporterXpress® Image Acquisition and Analysis Software, which features powerful image analysis capabilities and more than 25 available predefined experimental protocols, the ImageXpress Pico system becomes an extremely flexible device, ideally suited for user-defined, automated assays.
Illumination System Light Source The ImageXpress Pico system light source is comprised of six high-powered LEDs with a rated lifetime of more than 20,000 hours. It has an excitation spectrum ranging from ultraviolet to red. There are no user-replaceable parts in the light source.
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Chapter 1: ImageXpress Pico Automated Cell Imaging System Sample (X-Y) Stage Plate Holder The plate holder is designed for imaging multi-well microplates in standard ANSI (SBS) formats with plastic or glass bottoms and standard footprint dimensions. It can also be used with the optional dish holder, which holds up to six 35 mm (1.38 in.) dishes.
CellReporterXpress Software Features The CellReporterXpress Image Acquisition and Analysis Software is the user interface for the ImageXpress Pico system. You will use the CellReporterXpress software to work with the ImageXpress Pico system and control all its functions. The CellReporterXpress software integrates image acquisition and analysis into a unified workflow.
Chapter 1: ImageXpress Pico Automated Cell Imaging System Theory of Operation The ImageXpress Pico system uses the following components and functions: Fluorescence Imaging, see below Objective Lenses, see page 18 Filter Cubes, see page 19 Fluorescence Imaging Fluorescence is a property of certain classes of molecules (fluorophores) in which photons of a specific wavelength are absorbed (excitation), and, as a result, photons are emitted at a longer wavelength (emission) a very short time later.
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ImageXpress Pico Automated Cell Imaging System Installation Guide Objective Lenses The ImageXpress Pico system can be configured with high-quality Leica Fluotar objectives. Five objectives are currently available. You can identify the magnification of an objective by the color band: Objective Magnification...
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Chapter 1: ImageXpress Pico Automated Cell Imaging System Filter Cubes In the ImageXpress Pico system, the filter cube contains the excitation and emission filters and the dichroic mirror. These optical components are essential parts of an epi-illumination fluorescence imaging system in which the illumination and imaging optical paths overlap at the objective lens.
Our Support website, www.moleculardevices.com/service-support, describes the support options offered by Molecular Devices, including service plans and professional services. It also has a link to the Molecular Devices Knowledge Base, which contains documentation, technical notes, software upgrades, safety data sheets, and other resources.
ImageXpress Pico system or the CellReporterXpress software. About This Guide This guide is intended for the scientist or IT professional installing the ImageXpress Pico system. It describes the day-one activities and helps you set up the instrument, including the CellReporterXpress software.
Chapter 2: Setting Up the ImageXpress Pico System Package Contents The ImageXpress Pico system arrives in one box, which contains the instrument and its accessories. The package includes the following items: Item Description Installation Guide Two (2) CAT6 Ethernet Cables, 2 m (6.6 ft) One of the following: Power Cord for USA/Canada, 2.29 m (7.5 ft)
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ImageXpress Pico Automated Cell Imaging System Installation Guide Item Description Hardware Key Case, including the SafeNet Sentinel USB hardware key (also called a "dongle"), which is required to run the CellReporterXpress software With the optional environmental control system, the package also includes the following items:...
Molecular Devices for the ImageXpress Pico system. Environmental Requirements The ImageXpress Pico system is designed to operate indoors under laboratory conditions at 18°C to 30°C (64°F to 86°F) with 20% to 75% non-condensing humidity. As with any precision optical instrument, take care to maintain a low-dust, low-vibration environment. Temperature and humidity extremes can compromise performance.
ImageXpress Pico Automated Cell Imaging System Installation Guide Space and Table Requirements The ImageXpress Pico system requires a sturdy table or lab bench with a weight rating of at least 50 kg (110.2 lb). Note: With no options, the instrument weighs 38 kg (83.8 lb).
Chapter 2: Setting Up the ImageXpress Pico System Unpacking the Instrument Note: If you have chosen to have your instrument installed by a Molecular Devices field service engineer, do not open any boxes until the field service engineer is present (unless instructed to do so by Molecular Devices).
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ImageXpress Pico Automated Cell Imaging System Installation Guide 5. Grasp the handle on the cardboard and slide the instrument out of the box. Note: You may need another person to hold the box while you slide the instrument. 6. Remove the accessory boxes and the foam side supports.
Chapter 2: Setting Up the ImageXpress Pico System 8. With one person on each end, lift the instrument to a dry, flat surface. CAUTION! Lift the instrument from the bottom; avoid lifting from the top cover. Keep the instrument upright and level when lifting. Do not tip or shake the instrument.
Setting Up the Workstation and Monitor Bundle The optional workstation and monitor bundle includes a computer and monitor designed and set up as the host computer in the ImageXpress Pico system. The bundle includes the following: Dell Precision Tower...
Chapter 2: Setting Up the ImageXpress Pico System Connecting Cables The power cord and Ethernet cable connect to the ports on the rear of the instrument. The Ethernet port enables you to connect the instrument to the host computer running the CellReporterXpress software (in a standalone configuration) or to your network (in a network configuration or server configuration).
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ImageXpress Pico Automated Cell Imaging System Installation Guide Standalone Configuration To connect cables for a standalone configuration: 1. Do the following to connect the supplied Ethernet cable: a. Connect one end of the Ethernet cable to the LAN1 port on the instrument.
Chapter 2: Setting Up the ImageXpress Pico System Starting the System Use the following procedures to power on the ImageXpress Pico system. Powering On the Instrument, see below Powering On the Host Computer, see page 34 Powering On the Instrument To power on the instrument: 1.
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ImageXpress Pico Automated Cell Imaging System Installation Guide Powering On the Host Computer Power on the host computer and monitor, and log in to Windows. Note: You will also use the Windows user name and password to log in to the CellReporterXpress software.
ImageXpress Pico system and control its functions. After you set up the ImageXpress Pico system as described in the previous section, you are ready to install the CellReporterXpress software. See the CellReporterXpress Installation Guide for details.
Chapter 4: Finishing Setup of the ImageXpress Pico System Logging In to the Software Note: If you have just powered on or restarted the host computer, wait five minutes before logging in. This allows time for the CellReporterXpress software services to start.
Note: If the status indicator for an instrument shows Incompatible, the instrument firmware version may not be compatible with the software version. For details on updating the firmware, log in to the Molecular Devices Knowledge Base at mdc.custhelp.com and search for article number 20356.
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The instrument appears in the list on the My Devices tab. Tip: We recommend that you do not directly connect an instrument to the host computer using the LAN2 port unless advised to do so by Molecular Devices Technical Support.
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IP address of the instrument you want to add. Note: The instrument name begins with "IXP-" followed by the serial number, which is on the back of the instrument. See the ImageXpress Pico User Guide for details on locating the serial number.
With the instrument power on, do not manually rotate the objective turret. Manually rotating the objective turret can damage the instrument. Molecular Devices precalibrates the objectives to specific slots in the turret. You must install the objectives as follows: Slot...
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ImageXpress Pico Automated Cell Imaging System Installation Guide To install an objective: 1. In the CellReporterXpress software, on the Home page, click Devices. 2. Click Show Device Options to expand the details for the instrument where you want to install an objective.
Texas Red Note: Depending on how you have configured your ImageXpress Pico system, you may not have received all the filter cubes. Do not install a filter cube in slot 6 of the turret. Slot 6 must be empty (in both the instrument and the software).
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ImageXpress Pico Automated Cell Imaging System Installation Guide To install a filter cube: 1. In the CellReporterXpress software, on the Home page, click Devices. 2. Click Show Device Options to expand the details for the device where you want to install a filter cube.
Open the top door of the instrument to insert a labware holder in the sample stage. The ImageXpress Pico System includes a plate holder for standard multi-well microplates and a 4- slide holder for standard microscope slides. An optional 6-dish holder for standard 35 mm culture dishes is also available.
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ImageXpress Pico Automated Cell Imaging System Installation Guide Inserting the Plate Holder Like the sample stage, the plate holder also has an A1 label to indicate its top left corner. To insert the plate holder: 1. Press the button at the top right of the instrument to open the top door.
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Chapter 4: Finishing Setup of the ImageXpress Pico System Inserting the Slide Holder Like the sample stage, the slide holder also has an A1 label to indicate its top left corner. To insert the slide holder: 1. Press the button at the top right of the instrument to open the top door. 2.
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ImageXpress Pico Automated Cell Imaging System Installation Guide Inserting the Dish Holder The optional dish holder fits inside the plate holder in the sample stage. It can be inserted in either direction, so there is no label on it. To insert the dish holder: 1.
Chapter 5: Environmental Control System The optional environmental control system enables you to control the environment of your sample in a plate. You can regulate temperature, humidity, CO , and O inside the environmental control cassette to do the following: Keep cells alive for several days, growing at a rate comparable to that expected with a standard cell culture incubator.
WARNING! BIOHAZARD. It is your responsibility to decontaminate components of the instrument before you return parts to Molecular Devices for repair. Molecular Devices does not accept items that have not been decontaminated where it is applicable to do so. If parts are returned, they must be enclosed in a sealed plastic bag stating that the contents are safe to handle and are not contaminated.
Humidity Control: Provides 85% humidity by bubbling gases through the humidifying column to minimize evaporation from the sample labware over the duration of a time-lapse experiment. This section contains information on the following: Items Provided by Molecular Devices, see page 52 Items Provided by You, see page 53 5063666 K...
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Humidifying Column Tubing/Wiring: Connects the humidifying column to the instrument. Gas Supply Tubing: Molecular Devices provides 10 m (32.8 ft) of tubing to connect the instrument to the regulator. If this is not sufficient, you must provide an appropriate length of 4 mm I.D.
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Regulator Details on page 73 for details. Gas Supply Tubing: Molecular Devices provides 10 m (32.8 ft) of tubing to connect the instrument to the regulator. If this is not sufficient, you must provide an appropriate length of 4 mm I.D. / 6 mm O.D. polyurethane tubing.
When the status light on the Power button is amber, the instrument is fully powered off. Note: If the ImageXpress Pico system doesn't respond, you can perform a hard shutdown by holding the Power button for five seconds.
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Chapter 5: Environmental Control System 3. On the environmental control tray, loosen the large screw to remove the top piece from the tray. 4. On the bottom piece of the environmental control tray, slide the tab under the instrument so it engages the two screws. 5.
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ImageXpress Pico Automated Cell Imaging System Installation Guide 6. Do the following to connect the humidifying column: Note: See Connecting and Disconnecting Tubing on page 58 for details on connecting tubing. a. Align the red dots to connect the electronics cable to the lower port on the humidifying column.
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Chapter 5: Environmental Control System 7. Do the following to connect the humidifying column to the instrument: a. Connect the tubing with the white 1 band to the OUT WHITE 1 port. b. Connect the tubing with the yellow 2 band to the OUT YELLOW 2 port. c.
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ImageXpress Pico Automated Cell Imaging System Installation Guide Connecting and Disconnecting Tubing As part of working with the environmental control system, you must connect and disconnect tubing. WARNING! Do not connect or disconnect tubing when there is pressure from the gas cylinder.
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Chapter 5: Environmental Control System Filling the Humidifying Column The humidifying column uses ultrapure water to add humidity to the air flow supplied to the environmental control cassette. The humidifying column holds 130 ml (4.4 oz) of ultrapure water, which is enough to continuously provide humidity for up to three or four days.
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ImageXpress Pico Automated Cell Imaging System Installation Guide CAUTION! Do not operate humidity level control if the water level is below the minimum indicator. Operating without enough water can damage the instrument and the humidifying column. Before and during an experiment with humidity level control, check the water level in the humidifying column and refill as needed.
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Chapter 5: Environmental Control System Loading the Environmental Control Cassette The environmental control cassette is a magnetically sealed, glass enclosure that holds the plate or dishes in the instrument. Like the sample stage, both the environmental control cassette and lid also have an A1 label to indicate their top left corners.
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ImageXpress Pico Automated Cell Imaging System Installation Guide To load the environmental control cassette: 1. If the instrument is powered off, press the Power button on the front of the instrument to turn on the power. See Powering On the Instrument on page 33 for details.
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Chapter 5: Environmental Control System Using the Plate Skirt Height Adapter If you are using the environmental control cassette and autofocus fails, the CellReporterXpress software may display the following message: In this case, you may need to insert the plate skirt height adapter. The plate skirt height adapter sits inside the environmental control cassette to raise the plate, which can help autofocus succeed.
Maintaining the Environmental Control System User maintenance tasks for the environmental control system include running air dry and cleaning the humidifying column. See the ImageXpress Pico User Guide for details. Troubleshooting the Environmental Control System If you experience an issue with the environmental control system, review the troubleshooting tips in the ImageXpress Pico User Guide before contacting Molecular Devices Technical Support.
The instrument must be installed on a level and stable surface. WARNING! If the instrument is used in a manner not specified by Molecular Devices, the protection provided by the equipment might be impaired. The ImageXpress Pico system is an Equipment Class 1 product that relies on protective earth grounding for safe operation.
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ImageXpress Pico Automated Cell Imaging System Installation Guide Item Description Reading Modes Fluorescence Transmitted Light (Brightfield) Colorimetric Objectives 20x (optional) 40x (optional) 63x (optional) Fluorescence Color Channels FITC DAPI (optional) TRITC (optional) CY5 (optional) Texas Red (optional) CFP (optional) Environmental Control : 1% to 15% (optional)
Appendix A: Instrument Specifications Instrument Dimensions without Environmental Control System Front View Item Description 67.8 cm (26.7 in.) 35.3 cm (13.9 in.) 55.1 cm (21.7 in.) Side View Item Description 45.3 cm (17.8 in.) 39.7 cm (15.6 in.) 5063666 K...
ImageXpress Pico Automated Cell Imaging System Installation Guide Top View Item Description 42.5 cm (16.7 in.) 19.5 cm (7.7 in.) Instrument Dimensions with Environmental Control System Front View Item Description 67.8 cm (26.7 in.) 35.3 cm (13.9 in.) 62.9 cm (24.8 in.) 5063666 K...
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Appendix A: Instrument Specifications Side View Item Description 45.3 cm (17.8 in.) 49.0 cm (19.3 in.) Top View Item Description 51.6 cm (20.3 in.) 19.5 cm (7.7 in.) 5063666 K...
Appendix B: Optional Parts and Accessories This section describes the items available from Molecular Devices for the ImageXpress Pico system, including the following: Objectives, see below Filter Cubes, see below Accessories, see page 72 Obtaining Support on page 20 for details on contacting Molecular Devices.
ImageXpress Pico Automated Cell Imaging System Installation Guide Accessories The following accessories, which are available from Molecular Devices, are compatible with the ImageXpress Pico system. Base System Accessories Item Part No. 4-Slide Holder 5068503 Plate Holder 5068504 6-Dish Holder for 35 mm Dishes...
The connections required to connect the instrument are dependent on whether the gas is supplied from a compressed gas cylinder, a house air supply, or an air compressor. The ImageXpress Pico system can use the following gases: , which is typically supplied from a gas cylinder.
ImageXpress Pico Automated Cell Imaging System Installation Guide Using a Gas Cylinder Gas pressure to the instrument must be regulated within the range of 0.8 bar to 1.2 bar (11.6 psi to 17.4 psi). The ideal setting is 1.0 bar (14.5 psi). A two-stage regulator, as described below, is required to step-down and regulate the pressure from the gas cylinder.
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Appendix C: Environmental Control System Gas Regulator Details Connecting the Regulator to the Instrument Molecular Devices provides the following: Three (3) ¼" NPT male to 6mm O.D. push-to-connect fittings 10 m (32.8 ft) of 4 mm I.D. / 6 mm O.D. polyurethane tubing You may need to provide a ¼" x ¼" NPT female-to-female fitting (available from vendors such as Anderson Metals).
WARNING! All gases from the lab gas line must be oil-free. Failure to supply oil-free gases to the ImageXpress Pico system may damage the instrument and void the warranty. Note: We recommend that the delivery pressure range of the regulator be 4.14 bar (60 psig) or less, which will enable you to easily set the pressure to the required level.
ImageXpress Pico system may damage the instrument and void the warranty. An oil-free compressor can be purchased from many vendors, including Cole-Parmer (www.coleparmer.com). Unsupported Gas Supplies The following gas supplies are not supported for use with the ImageXpress Pico: Pre-mixed gas supplies boil-off from a liquid N dewar...
To pack the instrument: Note: Before you begin, if your instrument includes the optional environmental control system, run air dry to clear any condensation from the environmental control system. See the ImageXpress Pico User Guide for details. 5063666 K...
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ImageXpress Pico Automated Cell Imaging System Installation Guide 1. If needed, do the following to disassemble the environmental control system: a. Confirm that the valves on the tanks are completely shut off and that no gas is flowing. b. On the environmental control tray, loosen the large screw to remove the cover from the tray.
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Appendix D: Packing the Instrument for Transport 2. Remove any installed filter cubes and pack them in their original packaging. See Installing a Filter Cube on page 43 for details on removing a filter cube. 3. Remove any installed objectives and pack them in their original packaging, including the objective case.
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5. Press the Power button on the front of the instrument. When the status light on the Power button is amber, the instrument is fully powered off. Note: If the ImageXpress Pico system doesn't respond, you can perform a hard shutdown by holding the Power button for five seconds.
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CAUTION! Do not transport the instrument without properly inserting the foam transport lock. If needed, contact Molecular Devices for a replacement foam transport lock. b. Use shipping tape to secure the foam transport lock to ensure it does not come loose.
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ImageXpress Pico Automated Cell Imaging System Installation Guide 11. With one person on each end, place the instrument in the bottom foam packing as shown below. WARNING! LIFTING HAZARD. To prevent injury, use a minimum of two people to lift the instrument.
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Appendix D: Packing the Instrument for Transport 14. Insert the dongle case, the holders case, and the cables between the foam. 15. Slide the instrument on its cardboard base into the box. 16. Seal the box. 5063666 K...
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ImageXpress Pico Automated Cell Imaging System Installation Guide 17. Place the eight (8) edge protectors on the designated positions on the top edges of the box. 18. With one person on each end, place the box on the pallet. 19. Secure four (4) binding straps around the box and pallet.
Appendix E: Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Cet appareil ISM est confomre à la norme NMB-001 du Canada. ISM Equipment Classification (Group 1, Class A) This equipment is designated as scientific equipment for laboratory use that intentionally generate and/or use conductively coupled radio-frequency energy for internal functioning, and are suitable for use in all establishments, other than domestic and those directly connected to a low voltage power supply network which supply buildings used for domestic...
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